scholarly journals Evaluation of Knockdown Bioassay Methods to Assess Phosphine Resistance in the Red Flour Beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

Insects ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 140 ◽  
Author(s):  
Aaron Cato ◽  
Edwin Afful ◽  
Manoj K. Nayak ◽  
Thomas W. Phillips

Resistance to the fumigant phosphine in Tribolium castaneum occurs worldwide. This study evaluated tests based on adult knockdown time, the time for a beetle to become immobile, when exposed to a high concentration of phosphine. We recorded knockdown times of beetles that remained completely still for 30 s when exposed to 3000 ppm of phosphine in a large, gas-tight glass tube. Beetles were used from 12 populations, of which six were ‘susceptible’ to phosphine, three were ‘weakly resistant’, and three were ‘strongly resistant’. Knockdown times were determined for single beetles, as well as for groups of ten beetles for which the time to knockdown for either five beetles (KT50) or ten beetles (KT100) were recorded. Similar knockdown times occurred across susceptible and resistant populations. However, the KT100 tests generated conservative times for diagnosing strong vs. weak resistance. The strong resistant populations were all over 100 min with KT100, compared to 60 min or less for susceptible and weak resistant populations. Special tests on single beetles revealed higher knockdown times in insects that were deliberately disturbed compared to those without any disturbances. Work reported here suggests a knockdown test conducted on beetles in a matter of minutes or hours could help classify phosphine resistance status prior to decisions on phosphine fumigation.

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31582 ◽  
Author(s):  
Rajeswaran Jagadeesan ◽  
Patrick J. Collins ◽  
Gregory J. Daglish ◽  
Paul R. Ebert ◽  
David I. Schlipalius

2014 ◽  
Vol 27 (2) ◽  
pp. 1525-1527 ◽  
Author(s):  
Qiu-Ning Liu ◽  
Dan-Dan Bian ◽  
Sen-Hao Jiang ◽  
Zhen-Xing Li ◽  
Bao-Ming Ge ◽  
...  

2015 ◽  
Vol 42 (2) ◽  
pp. 211-216 ◽  
Author(s):  
Md Mostakim ◽  
Ataur Rahman Khan

Effect of various concentrations of coffee, Coffea arabica, viz. 12.50%, 25% and 50% on the growth and development of the red flour beetle, Tribolium castaneum (Herbst) was determined. Coffee lengthened the larval and pupal periods significantly. There was also a significant reduction in the pupal recovery and adult emergence of the beetle following treatment. The food with 50% coffee had the most detrimental effects on the beetle. Coffee extract could be used as useful botanical pesticide for controlling red flour beetle.Bangladesh J. Zool. 42(2): 211-216, 2014


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