scholarly journals Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging

2019 ◽  
Vol 5 (11) ◽  
pp. 83
Author(s):  
Qimei Zhang ◽  
Anna M. Grabowska ◽  
Philip A. Clarke ◽  
Stephen P. Morgan
Keyword(s):  
Fluorescence Imaging ◽  
Spatial Resolution ◽  
Near Infrared ◽  
Scanning System ◽  
Deep Tissue ◽  
Full Field ◽  
Infrared Wavelength ◽  
Tissue Optical Properties ◽  
Illumination System ◽  
Tissue Fluorescence

The spatial resolution and light detected in fluorescence imaging for small animals are limited by light scattering, absorption and autofluorescence. To address this, novel near-infrared fluorescent contrast agents and imaging configurations have been investigated. In this paper, the influence of the light wavelength and imaging configurations (full-field illumination system and scanning system) on fluorescence imaging are compared quantitatively. The surface radiance for both systems is calculated by modifying the simulation tool Near-Infrared Fluorescence and Spectral Tomography. Fluorescent targets are embedded within a scattering medium at different positions. The surface radiance and spatial resolution are obtained for emission wavelengths between 620 nm and 1000 nm. It was found that the spatial resolution of the scanning system is independent of the tissue optical properties, whereas for full-field illumination, the spatial resolution degrades at longer wavelength. The full width at half maximum obtained by the scanning system is 25% lower than that obtained by the full-field illumination system when the targets are located in the middle of the phantom. The results indicate that although imaging at near-infrared wavelength can achieve a higher surface radiance, it may produce worse spatial resolution.

Deep-tissue Fluorescence Imaging using Ultrasound-encoded Digitally Time-reversed Light

2012 ◽  
Author(s):  
Ying Min Wang ◽  
Benjamin Judkewitz ◽  
Charles A. DiMarzio ◽  
Changhuei Yang
Keyword(s):  
Fluorescence Imaging ◽  
Deep Tissue ◽  
Tissue Fluorescence

scholarly journals 3D Tissue Reconstruction by using Deep Tissue Fluorescence Imaging System and FLIM

2014 ◽  
Vol 106 (2) ◽  
pp. 400a
Author(s):  
Sohail Jahid ◽  
Alexander S. Dvornikov ◽  
Michelle Digman ◽  
Enrico Gratton
Keyword(s):  
Fluorescence Imaging ◽  
Imaging System ◽  
Deep Tissue ◽  
Tissue Reconstruction ◽  
Fluorescence Imaging System ◽  
Tissue Fluorescence

scholarly journals Deep tissue fluorescence imaging andin vivobiological applications

2012 ◽  
Vol 17 (11) ◽  
pp. 116023 ◽  
Author(s):  
Viera Crosignani ◽  
Alexander Dvornikov ◽  
Jose S Aguilar ◽  
Chiara Stringari ◽  
Robert Edwards ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Deep Tissue ◽  
Tissue Fluorescence

scholarly journals Protein enhanced NIR-IIb emission of indocyanine green for functional bioimaging

2020 ◽  
Author(s):  
Mubin He ◽  
Di Wu ◽  
Yuhuang Zhang ◽  
Xiaoxiao Fan ◽  
Hui Lin ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Clinical Application ◽  
Spatial Resolution ◽  
Near Infrared ◽  
Protein Solutions ◽  
Lymph System ◽  
Fetal Bovine ◽  
Auto Fluorescence

AbstractFluorescence imaging performed in the 1500-1700 nm spectral range (labeled as near-infrared IIb, NIR-IIb) promises high imaging contrast and spatial resolution for its little photon scattering effect and minimum auto-fluorescence. Though inorganic and organic probes have been developed for NIR-IIb bioimaging, most are in preclinical stage, hampering further clinical application. Herein, we showed that indocyanine green (ICG), an US Food and Drug Administration (FDA)-approved agent, exhibited remarkable amount of NIR-IIb emission when dissolved into different protein solutions, including human serum albumin, rat bile, and fetal bovine serum. We performed fluorescence imaging in NIR-IIb window to visualize structures of lymph system, extrahepatic biliary tract and cerebrovascular. Results demonstrated that proteins promoted NIR-IIb emission of ICG in vivo and that NIR-IIb imaging with ICG preserved higher signal-to-background ratio (SBR) and spatial resolution compared with the conventional near-infrared II (NIR-II) fluorescence imaging. Our findings confirm that NIR-IIb fluorescence imaging can be successfully performed using the clinically approved agent ICG. Further clinical application in NIR-IIb region would hopefully be carried out with appropriate ICG-protein solutions.

scholarly journals Near-Infrared IIb Fluorescence Imaging of Vascular Regeneration with Dynamic Tissue Perfusion Measurement and High Spatial Resolution

2018 ◽  
Vol 28 (36) ◽  
pp. 1803417 ◽  
Author(s):  
Zhuoran Ma ◽  
Mingxi Zhang ◽  
Jingying Yue ◽  
Cynthia Alcazar ◽  
Yeteng Zhong ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Spatial Resolution ◽  
Near Infrared ◽  
High Spatial Resolution ◽  
Tissue Perfusion ◽  
Perfusion Measurement ◽  
Vascular Regeneration

Acceptor Engineering of Small Molecule Fluorophores for NIR-II Fluorescent and NIR-I Photoacoustic Imaging

2021 ◽  
Author(s):  
Yaxi Li ◽  
Hongli Zhou ◽  
Renzhe Bi ◽  
Xiuting Li ◽  
Menglei Zha ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Small Molecule ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
High Sensitivity ◽  
Tissue Penetration ◽  
Deep Tissue ◽  
Infrared Window

Fluorescence imaging in the second near-infrared window (NIR-II) has been an emerging technique in diverse in vivo applications with high sensitivity/resolution and deep tissue penetration. To date, the designing principle...

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