Evaluation of the Association of Omentin 1 rs2274907 A>T and rs2274908 G>A Gene Polymorphisms with Coronary Artery Disease in Indian Population: A Case Control Study

Coronary artery disease (CAD) is a major cause of death all over the world. CAD is caused by atherosclerosis which is induced by the interaction of genetic factors and environmental factors. Traditional environmental risk factors include hyperlipidemia, diabetes mellitus, lack of exercise, obesity, poor diet and others. Genome-wide association studies have revealed the association of certain gene polymorphisms with susceptibility to CAD. Omentin 1 is an adipokine secreted by the visceral adipose tissues and has been reported to have anti-inflammatory, cardioprotective, and enhances insulin sensitivity. In this study, we examined the role of omentin-1 common single nucleotide polymorphisms (SNPs) (rs2274907 A>T and rs2274908 G>A) in CAD. We genotyped 100 CAD patients and 100 matched healthy controls from the south Indian population using an amplification refractory mutation system (ARMS-PCR) and allele-specific PCR (AS-PCR). Our result indicated the rs2274908 G>A is not associated with CAD. Results showed that there was a significant difference in rs2274907 A>T genotype distribution between controls and CAD cases (P-value < 0.05). Results indicated that the AT genotype of the rs2274907 is associated with CAD with OR = 3.0 (95% confidence interval (CI), 1.64 to 5.49), 1.65 (1.27 to 2.163), P = 0.002. The T allele of the rs2274907 was also associated with CAD with OR = 1.82 (95% CI, 1.193 to 2.80), 1.37 (1.08 to 1.74), P = 0.005. Rs2274907 genotype distribution was also correlated with serum total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), hypertension and diabetes. We conclude that the AT genotype and the T allele of the rs2274907 A>T is associated with Cad in the south Indian population. Further studies on the effect of the rs2274907 A>T on omentin-1 function are recommended, and future well-designed studies with larger sample sizes and in different populations are required to validate our findings.

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Reply to Comment: Evaluation of the Association of Omentin 1 rs2274907 A > T and rs2274908 G > A Gene Polymorphisms with Coronary Artery Disease in Indian Population: A Case–Control Study

Journal of Personalized Medicine ◽

10.3390/jpm10040194 ◽

2020 ◽

Vol 10 (4) ◽

pp. 194

Author(s):

Chandan K. Jha ◽

Rashid Mir ◽

Imadeldin Elfaki ◽

Jamsheed Javid ◽

Abdullatif Taha Babakr ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Gene Polymorphisms ◽

Indian Population ◽

Association Studies ◽

Large Sample Size ◽

South Indian Population ◽

Genome Wide Association Studies ◽

South Indian ◽

Artery Disease

Coronary artery disease (CAD) is a major cause of death all over the world. CAD is caused by atherosclerosis which is induced by the interaction of genetic factors and environmental factors. Genome-wide association studies have revealed the association of certain gene polymorphisms with susceptibility to CAD. Omentin 1 is an adipokine secreted by the visceral adipose tissues and has been reported to have anti-inflammatory, cardioprotective, and enhances insulin sensitivity. In this study, we examined the role of omentin-1 common single nucleotide polymorphisms (SNPs) (rs2274907 A > T and rs2274908 G > A) in CAD. We conclude that the AT genotype and the T allele of the rs2274907 A > T is associated with Cad in the south Indian population. Our results indicated that the rs2274907 SNP may be associated with CAD in this population. This finding needs further validation in well-designed and large-sample size studies before being introduced in clinical settings.

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PW220 The Prevalence of Metabolic syndrome in Coronary artery Disease patients compared to those without Coronary Artery Disease: Insights from South Indian population

Global Heart ◽

10.1016/j.gheart.2014.03.2318 ◽

2014 ◽

Vol 9 (1) ◽

pp. e302

Author(s):

Stigimon Joseph

Keyword(s):

Coronary Artery Disease ◽

Metabolic Syndrome ◽

Coronary Artery ◽

Indian Population ◽

South Indian Population ◽

South Indian ◽

Artery Disease

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Letter to the editor regarding “Role of cytochrome epoxygenase (CYP2J2) in the pathophysiology of coronary artery disease in South Indian Population.”

Indian Heart Journal ◽

10.1016/j.ihj.2019.02.001 ◽

2019 ◽

Vol 71 (2) ◽

pp. 174-175 ◽

Cited By ~ 1

Author(s):

Ashish Kumar U ◽

Mariam Shariff

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Indian Population ◽

South Indian Population ◽

Letter To The Editor ◽

South Indian ◽

Artery Disease

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LDLR Gene Polymorphisms (rs5925 and rs1529729) Are Associated with Susceptibility to Coronary Artery Disease in a South Indian Population

Medical Sciences ◽

10.3390/medsci7070080 ◽

2019 ◽

Vol 7 (7) ◽

pp. 80 ◽

Cited By ~ 1

Author(s):

Chandan K. Jha ◽

Rashid Mir ◽

Imadeldin Elfaki ◽

Shaheena Banu ◽

S. M. S. Chahal

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Large Scale ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Cholesterol Homeostasis ◽

South Indian Population ◽

Specific Pcr ◽

South Indian ◽

Artery Disease

Cardiovascular diseases (CVD) are a major cause of death in India and worldwide. Atherosclerosis is caused by the interaction of environmental and genetic factors. Hypercholesterolemia is an example of a classical risk factor for CVD. The low-density lipoprotein receptor (LDLR) is one of the regulating mechanisms the liver uses for cholesterol homeostasis. Gene variations in the LDLR have been reported to cause hypercholesterolemia and consequently CVD. We investigated the association of polymorphisms in the LDLR (rs5925 and rs1529729) with coronary artery disease (CAD) in 200 coronary artery disease patients and 200 matched healthy controls using allele-specific PCR (AS-PCR). The results indicated that the CT genotype of the rs1529729 polymorphism was associated a decreased susceptibility to CAD with an odds ratio (OR) = 0.42 (95% confidence interval (CI), 0.23–0.77), risk ratio (RR) = 0.59 (0.39–0.89), P = 0.0047. The TT genotype of the rs1529729 polymorphism was also associated with decreased susceptibility to CAD with an OR = 0.19 (95% CI, 0.076–0.47), RR = 0.57 (0.47–0.69), P = 0.0003. The GA genotype of the rs5925 polymorphism was associated with decreased susceptibility to CAD with an OR = 0.45 (95% CI, 0.27–0.75), RR = 0.65 (0.47–0.88), P = 0.002. We concluded that the CT and TT genotypes of the rs1529729 polymorphism and the GA genotype of the rs5925 polymorphism are probably associated with decreased susceptibility to CAD. The simplicity of AS-PCR makes it particularly suitable for the rapid, large-scale screening of gene variabilities in the LDLR. AS-PCR could provide significant benefits in clinical applications with its ability to amplify a lower quantity of samples in a cost-saving manner. Nevertheless, these findings need to be validated in well-designed studies with larger sample sizes and in different populations.

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