scholarly journals SARS-CoV-2 Detection in Fecal Sample from a Patient with Typical Findings of COVID-19 Pneumonia on CT but Negative to Multiple SARS-CoV-2 RT-PCR Tests on Oropharyngeal and Nasopharyngeal Swab Samples

Medicina ◽  
2021 ◽  
Vol 57 (3) ◽  
pp. 290
Author(s):  
Barbara Brogna ◽  
Carlo Brogna ◽  
Mauro Petrillo ◽  
Adriana Modestina Conte ◽  
Giulio Benincasa ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Clinical Symptoms ◽  
Nasopharyngeal Swab ◽  
Upper Respiratory Tract ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Negative Results ◽  
Upper Respiratory ◽  
Polymerase Chain ◽  
Pcr Test

Reverse transcriptase polymerase chain reaction (RT-PCR) negative results in the upper respiratory tract represent a major concern for the clinical management of coronavirus disease 2019 (COVID-19) patients. Herein, we report the case of a 43-years-old man with a strong clinical suspicion of COVID-19, who resulted in being negative to multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RT-PCR tests performed on different oropharyngeal and nasopharyngeal swabs, despite serology having confirmed the presence of SARS-CoV-2 IgM. The patient underwent a chest computed tomography (CT) that showed typical imaging findings of COVID-19 pneumonia. The presence of viral SARS-CoV-2 was confirmed only by performing a SARS-CoV-2 RT-PCR test on stool. Performing of SARS-CoV-2 RT-PCR test on fecal samples can be a rapid and useful approach to confirm COVID-19 diagnosis in cases where there is an apparent discrepancy between COVID-19 clinical symptoms coupled with chest CT and SARS-CoV-2 RT-PCR tests’ results on samples from the upper respiratory tract.

scholarly journals Amplification of human β-glucoronidase gene for appraising the accuracy of negative SARS-CoV-2 RT-PCR results in upper respiratory tract specimens

2020 ◽  
Author(s):  
Eliseo Albert ◽  
Blanca Ferrer ◽  
Ignacio Torres ◽  
Alicia Serrano ◽  
Maria Jesus Alcaraz ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Operating Characteristic ◽  
False Negative ◽  
Upper Respiratory Tract ◽  
Rt Pcr ◽  
Roc Curve Analysis ◽  
True Negative ◽  
Negative Results ◽  
Upper Respiratory ◽  
Polymerase Chain

Real-time reverse transcription polymerase-chain reaction (RT-PCR) is the mainstay of Covid-19 diagnosis. False-negative RT-PCR results may hamper clinical management of patients and hinder the adoption of epidemiological measures to control the pandemic. The current study was aimed at assessing whether amplification of β-glucoronidase (GUSB) gene would help estimate the accuracy of SARS-CoV-2 RT-PCR negative results in upper respiratory tract (URT) specimens. URT specimens that tested negative by SARS-CoV-2 RT-PCR displayed higher GUSB RT-PCR cycle thresholds (CT) (P=0.070) than those testing positive (median, 30.7; range, 27.0-40.0, and median 29.7; range 25.5-36.8, respectively), this reflecting poorer cellularity. Receiver operating characteristic (roc) curve analysis indicated that a CT threshold of 31.2 discriminated best between positive and negative SARS CoV-2 RT-PCRs (area under a curve, 0.66; 95% CI, 0.50-0.81; P=0.08). This cut-off yielded a true negative ratio of 89% and accuracy of 70%. The data suggested that amplification of the GUSB gene by RT-PCR may help to appraise the accuracy of negative SARS-CoV-2 RT-PCR results in patients in whom Covid-19 is eventually diagnosed.

scholarly journals Reducing False Negative PCR Test for COVID-19

2020 ◽  
Vol 9 (3) ◽  
pp. 408-410
Author(s):  
Fatemeh Bahreini ◽  
Rezvan Najafi ◽  
Razieh Amini ◽  
Salman Khazaei ◽  
Saeid Bashirian
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
False Negative ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Creative Commons ◽  
Negative Results ◽  
False Negative Results ◽  
Polymerase Chain ◽  
Pcr Test

As the SARS-CoV-2 (COVID-19) pandemic spreads rapidly, there is need for a diagnostic test with high accuracy to detect infected individuals especially those without symptoms. Real-time polymerase chain reaction (RT-PCR) is a common molecular test for diagnosing SARS-CoV-2. If some factors are not taken into consideration when performing this test, it can have a relatively large number of false negative results. In this article, we discuss important considerations that could lead to false negative test reduction. Key words: • SARS-CoV-2 • COVID-19 • Real time polymerase chain reaction • RT-PCR test • Diagnosis • False negatives • Genetics • Emerging disease   Copyright © 2020 Bahreini et al. Published by Global Health and Education Projects, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0)which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in this journal, is properly cited.

scholarly journals The clinical sensitivity of a single SARS-CoV-2 upper respiratory tract RT-PCR test for diagnosing COVID-19 using convalescent antibody as a comparator

2020 ◽  
Vol 20 (6) ◽  
pp. e209-e211 ◽  
Author(s):  
Abigail Holborow ◽  
Hibo Asad ◽  
Lavinia Porter ◽  
Poppy Tidswell ◽  
Claire Johnston ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Upper Respiratory Tract ◽  
Rt Pcr ◽  
Clinical Sensitivity ◽  
Upper Respiratory ◽  
Pcr Test

scholarly journals Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

2014 ◽  
Vol 61 (4) ◽  
Author(s):  
Anna Biernasiuk ◽  
Izabela Korona-Głowniak ◽  
Agnieszka Grzegorczyk ◽  
Anna Malm
Keyword(s):  
Respiratory Tract ◽  
Dna Polymerase ◽  
Random Amplified Polymorphic Dna ◽  
Small Cell ◽  
Upper Respiratory Tract ◽  
Small Cell Lung ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Upper Respiratory ◽  
Polymerase Chain

Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.

COVID-19 Reinfection in a Healthcare Worker; Is There a Definitive Immunity Against SARS-CoV-2?

2021 ◽  
Author(s):  
Sayyed Reza Ahmadi ◽  
Saeideh Anvari Ardakani ◽  
Navid Kalani ◽  
Seyed Reza Habibzadeh ◽  
Elnaz Vafadar Moradi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Healthcare Worker ◽  
Nasopharyngeal Swab ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Case Presentation ◽  
Long Period ◽  
Polymerase Chain ◽  
Pcr Test ◽  
First Admission

Introduction: While our knowledge is limited about COVID-19 immunity, recent cases of reinfection have raised concerns. Case presentation: Here, we report a case of COVID-19 reinfection after three months from recovery in a healthcare worker with negative IgM and IgG at the second infection and positive nasopharyngeal swab Reverse transcription polymerase chain reaction (RT-PCR) test despite being discharged with two negative RT-PCR tests at the first admission. Symptoms at first admission were fever, headache, sore throat, diarrhea, and vomiting and got changed to myalgia and anosmia. Conclusion: The strength of this case report is the long period (three months) between the infection and reinfection while other cases reported in literature were reinfected less than one month after their first infection.

scholarly journals CT FINDINGS IN INITIAL RT-PCR NEGATIVE TESTING COVID 19: BASED ON STUDY IN A LARGEST TERTIARY CARE CENTRE OF CENTRAL INDIA.

2020 ◽  
pp. 33-36
Author(s):  
Shyam Chhadi ◽  
Aarti Anand ◽  
Ravi Kumar
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Ground Glass ◽  
Imaging Findings ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Negative Results ◽  
Polymerase Chain ◽  
Ct Features ◽  
Pcr Test

SUMMARY STATEMENT CT imaging findings in patients of suspected 2019-nCoV infection, who have initial negative results of reverse transcription–polymerase chain reaction (RT-PCR) testing. OBJECTIVE The purpose of this study is to explore the diagnostic value of CT over RT-PCR in the diagnosis of corona virus disease (COVID-19) pneumonia, especially for patients who have initial negative results of reverse transcription–polymerase chain reaction (RT-PCR) testing. MATERIALS AND METHODS Patients with suspected COVID-19 pneumonia from April 1, 2020, to September 30, 2020, were included. They are initially underwent RT-PCR followed by HRCT thorax after 1 day of negative initial RT-PCR testing. The imaging findings were obtained and compared with CT findings of those patients who have confirmed initial reverse transcription–polymerase chain reaction (RT-PCR) testing. RESULTS Total sixty (40 men, 20 women) patients who have initial negative RT-PCR testing but are highly suspicious for 2019-nCoV infection were included in our study and their age range between 22 years to 70 years. In our study most of the findings were seen involved the multiple( 70%) lobes in both lungs. The main CT features were ground-glass opacity (90%) and consolidation (60%) with a sub pleural (100%) and peri hilar or central distribution. The other CT features included air bronchograms (60%), vascular enlargement and interlobular septal thickening (50%), tree in bud appearance and pleural effusions (10%). when CT of patient with initial negative RT-PCR test were compared with CT of patients with initial positive RT-PCR test , it was found that most of the findings like ground glass opacities and consolidatory changes were also likely present in these group (p>0.05). CONCLUSION Chest CT is an important tools for screening and diagnosing the patients who are initial negative RT-PCR however they are highly suspicious and having symptoms like 2019-nCoV infection.

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