Effect of Temperature on Biobeneficiation of Bulk Copper-Nickel Concentrate with Thermoacidophilic Microbial Communities

Bioleaching of the bulk copper–nickel sulfide concentrate was proposed as a method to remove nickel from it and to obtain a concentrate containing copper as chalcopyrite. This approach is based on the different refractoriness of sulfide minerals in ferric sulfate solutions and oxidation by acidophilic microorganisms. The bulk concentrate contained 10.8% copper in the form of chalcopyrite (CuFeS2) and 7.2% nickel that occurred in pentlandite ((Ni,Fe)9S8) and violarite (FeNi2S4). Three microbial communities grown at 35, 40, and 50 °C were used for bioleaching. The microbial community at 40 °C was the most diverse in the genus and species composition. At all temperatures of the process, the key roles in bioleaching belonged to mixotrophic and heterotrophic acidophiles. The highest levels of nickel leaching of 97.2 and 96.3% were observed in the case of communities growing at 40 and 50 °C, respectively. At the same time, the bioleach residue, which could be characterized as a marketable high-grade copper (chalcopyrite) concentrate, was obtained only at 40 °C. This solid contained 15.6% copper and 0.54% nickel. Thus, the biobeneficiation of bulk sulfide concentrates can be a promising field of biohydrometallurgy.