scholarly journals Isolation and Characterization of Beneficial Bacteria from Food Process Wastes

2021 ◽  
Vol 9 (6) ◽  
pp. 1156
Author(s):  
A-Leum Kim ◽  
Seunghye Park ◽  
Yoon-Kyoung Hong ◽  
Ji-Hwan Shin ◽  
Se-Hwan Joo
Keyword(s):  
Food Waste ◽  
High Throughput Sequencing ◽  
Microbial Community Composition ◽  
Waste Recycling ◽  
Organic Content ◽  
Plant Growth Promoting Bacteria ◽  
Acetic Acid Production ◽  
Process Waste ◽  
Isolation And Characterization ◽  
Plant Growth Promoting

Significant quantities of food waste are accumulated globally on an annual basis, with approximately one-third of the food produced (equivalent to 1.3 billion tons of food) being wasted each year. A potential food waste recycling application is its utilization as a soil conditioner or fertilizer, whereby it increases the soil organic content and microbial biomass. This study evaluated the effectiveness of food waste as a microbial resource by analyzing the microbial community composition and isolating plant growth-promoting bacteria (PGPB) in food waste obtained from various sources. High-throughput sequencing identified 393 bacterial operational taxonomic units in the food process waste (FPW) samples. Moreover, the results showed that Firmicutes was abundant in the waste samples, followed by Bacteroidetes and Proteobacteria. A total of 92 bacteria were isolated from FPW. Moreover, the cultivable strains isolated from FPW belonged to the genus Bacillus, followed by Streptomyces and Proteus. Six isolated bacteria exhibited beneficial traits, including indole acetic acid production, antifungal resistance and extracellular lysis. FPW is a valuable microbial resource for isolation of PGPB, and its use as a fertilizer may enable a reduction in chemical fertilizer usage, thereby mitigating the corresponding adverse environmental impacts on sustainable crop development.

scholarly journals Isolation and Characterization of Potassium-Solubilizing Bacteria from Two Different Rhizospheres and a Cow Manure in IPB University

2021 ◽  
Vol 21 (2) ◽  
pp. 336
Author(s):  
Dori Kusuma Jaya ◽  
Sari Yulia Kartika Hasibuan ◽  
Deseriana Bria
Keyword(s):  
Tobacco Leave ◽  
Plant Growth Promoting Bacteria ◽  
Cow Manure ◽  
Plant Host ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Potassium Solubilizing Bacteria ◽  
Continuous Use

Applying PGPB (Plant Growth-Promoting Bacteria) as bioinoculant under the excessive and continuous use of chemical fertilizer, pesticide, and herbicide need to be highlighted nowadays. One of the most beneficial PGPB is potassium-solubilizing rhizobacteria. Potassium is one of the elements that plant absorbs in a large amounts along with nitrogen. Soil in various regions of Indonesia have low potassium solubility. Therefore, finding the best novel isolates contributing to potassium solubilization need to be carried out continually. The aim of this study is to find potassium-solubilizing bacteria from two different rhizospheres (banana and chili plant) in Leuwikopo Experimental Garden and a cow manure in Cattle Pen, IPB University. To obtain the potassium-solubilizing isolates, we observed and characterized the best growing isolates and measured the solubilizing zone on Alexandrov agar medium. Three out of 10 isolates were selected and tested their pathogenicity on tobacco leave. Solubilization index of three isolates from the highest to the lowest were MPK P (3.07), MPK KT (3.03), and MPK C (2.63), respectively. Necrotic on tobacco leave after isolates treatment showed negative result which means that the three isolates were not pathogenic to plant host. The isolates are recommended to be used as bioinoculant both in greenhouse and field plants which lack of potassium availability in soil.

scholarly journals Isolation and Characterization of Novel Plant Growth-Promoting Bacteria from the Fronds of Duckweed

2021 ◽  
Vol 57 (1) ◽  
pp. 1-9
Author(s):  
TOMOKI IWASHITA ◽  
YASUHIRO TANAKA ◽  
HIDEYUKI TAMAKI ◽  
RYOSUKE NAKAI ◽  
YASUKO YONEDA ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Bacteria ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant Growth-Promoting Bacteria Associated with the Nests of the Seed-Harvester Ant, Trichomyrmex Scabriceps

2022 ◽  
Author(s):  
Pooja Kumari ◽  
Neelkamal Rastogi ◽  
Harikesh Bahadur Singh ◽  
Rahul Singh Rajput
Keyword(s):  
Plant Growth ◽  
Sclerotium Rolfsii ◽  
Cultivated Plants ◽  
Plant Growth Promoting Bacteria ◽  
Harvester Ants ◽  
Acetic Acid Production ◽  
Organic Debris ◽  
Harvester Ant ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Colonies of seed harvester ants are commonly found in semiarid and arid areas of the world and have been studied for their seed dispersal behaviour. The present study focused on the bacteria associated with the nests of the harvester ant, Trichomyrmex scabriceps, and reveals that ant colonies link the aboveground resources with the belowground microbial communities as they accumulate organic debris in the close vicinity of their nests via their ecosystem engineering activities. Soil samples were collected from the nest chambers and the external debris piles of T. scabriceps colonies, located in managed ecosystems. The nest soil-associated bacteria were examined for their plant growth-promoting abilities via biochemical assays including phosphate solubilization, Indole acetic acid production, siderophore production and physiological assays including biocontrol potential against the soil pathogen, Sclerotium rolfsii. More than 60% of bacteria isolated from the ant nest-associated soil displayed plant-growth promoting ability. Bacillus sp., Azotobacter sp., Klebsiella sp., Comamonas sp., Tsukamurella sp., and Pseudoxanthomonax sp., demonstrated significantly high levels of gnotobiotic growth of the treated chickpea plants. The activities of phenylalanine ammonia-lyase and peroxidase enzymes were higher in plant growth-promoting bacteria treated and pathogen inoculated plants as compared to the control plants lacking the bacteria. Since T. scabriceps colonies often make their nests in the compact soil of unpaved paths of agroecosystems and gardens, these bacteria can act as highly effective biofertilizers and promote growth of the cultivated plants by increasing soil fertility and disease resistance attributes of the plant.

scholarly journals Isolation and Characterization of Plant Growth Promoting Bacteria Isolated from Andean Soil as Potential Inoculants of Soybean Seeds

2018 ◽  
Vol 8 (3) ◽  
pp. 203
Author(s):  
Carolina Belfiore ◽  
Ana P. Santos ◽  
Manuel Contreras ◽  
Maria E. Farias
Keyword(s):  
Plant Growth ◽  
Biochemical Characterization ◽  
Stem Length ◽  
Plant Growth Promoting Bacteria ◽  
Productive Capacity ◽  
Chemical Fertilizers ◽  
Isolation And Characterization ◽  
Protease Enzyme ◽  
Plant Growth Promoting ◽  
Growth Promoting

Argentina is the leading exporter of soybean oil and flour, and the third largest producer of grain. Since, the crop is a matter of great importance to the national economy. Their production depends on the soil as their main resource to ensure a good productive capacity, so it is necessary to preserve the physical, chemical and biological properties of the soil. Although, the indiscriminate use of chemical fertilizers, disturb them. In recent years, there has been a trend towards cleaner production to reduce the use of chemical. One of the alternatives involves biological means through the use of plant growth promoting bacteria. These group of bacteria colonize the rhizosphere of plants and stimulate the plant growth by several mechanisms. The objective of this work was to characterize, identify and evaluate the growth promoting effect of 13 strains isolated from the Andean vegetation rhizosphere. The bacterial isolates were Enterobacteria, Stenotrophomonas, Pseudomonas, Nocardiodes, Bacillus, Exiguobacterium, Acinetobacter and Lactococcus genera. The results of the biochemical characterization determined that from the 13 bacterial strains, which produce siderophores, 11 possess the catalase enzyme, 10 fixate nitrogen, 12 produce the protease enzyme, 12 solubilize phosphorus, and 11 produce indoleacetic acid. The application of different inoculums to the seeds, allowed to obtain plants with longer stem length, more developed roots, larger and more intense coloration leaves than the control plants. The results encourage deeper studies to achieve the formulation of inoculums to use as a biofertilizer, which would replace chemical fertilizers or reduce their doses.

scholarly journals Grape cultivar features differentiate the grape rhizosphere of microbiota

2020 ◽  
Author(s):  
Zhiguang Yang ◽  
Bo Sun ◽  
Shiwei Zhang ◽  
Lijun Bao ◽  
Yingxue Wei ◽  
...  
Keyword(s):  
Discriminant Analysis ◽  
Linear Discriminant Analysis ◽  
High Throughput Sequencing ◽  
Northern China ◽  
Pinot Noir ◽  
Plant Growth Promoting Bacteria ◽  
Linear Discriminant ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Crop Health

Abstract Background: Despite its importance to crop health and plant quality, the structure and diversity of the grape rhizosphere remains poorly described. In this study, the microflora community structures of grapes in northern China were compared using high-throughput sequencing. Results: We found that richness, diversity of bacterial and fungi community networking in the root compartments were significantly influenced by the grape variety. The bacterial linear discriminant analysis showed that Pseudomonas , Rhizobium which considered as potential plant-growth-promoting bacteria were more enriched in Pinot noir, and potential ammonia-oxidizing Nitrosospira was enriched in Gem. The fungal linear discriminant analysis showed that Fusarium was more enriched in Longan, Sporormiella more enriched in Merlot while Gibberella and Pseudallescheria were more enriched in Gem, and Mortierella was more abundant in Cabernet Sauvignon. Conclusions: The 16S rRNA functional prediction indicated that no significance differentiate among the varieties. The results showed that may be we can extinguish the varieties according to these characteristic bacterial and fungal genus.

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