Deconstructing Wine Grape Cell Walls with Enzymes During Winemaking: New Insights from Glycan Microarray Technology

Enzyme-aid maceration is carried out in most modern winemaking industries with a range of positive impacts on wine production. However, inconsistencies in enzyme efficiency are an issue complicated by unclear targets (limited information available on berry cell wall architecture of different cultivars) and the complex wine environment (i.e., fermenting must). Recent studies have been performed to develop a clearer picture of grape cell wall structures, maceration effects, and interactions between important wine compounds and grape-derived polysaccharides. This review highlights critically important recent studies on grape berry cell wall changes during ripening, the importance of enzymes during maceration (skin contact phase) and deconstruction processes that occur during alcoholic fermentation. The novelty of the Comprehensive Microarray Polymer Profiling (CoMPP) technique using cell wall probes (e.g., antibodies) as a method for following cell wall derived polymers during different biological and biotechnological processes is discussed. Recent studies, using CoMPP together with classical analytical methods, confirmed the developmental pattern of berry cell wall changes (at the polymer level) during grape ripening. This innovative technique were also used to track enzyme-assisted depectination of grape skins during wine fermentation and determine how this influence the release of wine favourable compounds. Furthermore, polysaccharides (e.g., arabinogalactan proteins) present in the final wine could be identified. Overall, CoMPP provides a much more enriched series of datasets compared to traditional approaches. Novel insights and future studies investigating grape cell wall and polyphenol interactions, and the tailoring of enzyme cocktails for consistent, effective and “customized” winemaking is advanced and discussed.

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SEM and fluorescence imaging of callose deposition in root hair tips and suspension cultures of Streptanthus tortuosus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161047 ◽

1990 ◽

Vol 48 (3) ◽

pp. 698-699

Author(s):

K.S. Walters ◽

R.D. Sjolund ◽

K.C. Moore

Keyword(s):

Cell Wall ◽

Root Hair ◽

Cultured Cells ◽

Root Hairs ◽

Callus Cultures ◽

Callose Deposition ◽

Culture Cells ◽

Wall Structures ◽

Ultraviolet Illumination ◽

Callose Formation

Callose, B-1,3-glucan, a component of cell walls, is associated with phloem sieve plates, plasmodesmata, and other cell wall structures that are formed in response to wounding or infection. Callose reacts with aniline blue to form a fluorescent complex that can be recognized in the light microscope with ultraviolet illumination. We have identified callose in cell wall protuberances that are formed spontaneously in suspension-cultured cells of S. tortuosus and in the tips of root hairs formed in sterile callus cultures of S. tortuosus. Callose deposits in root hairs are restricted to root hair tips which appear to be damaged or deformed, while normal root hair tips lack callose deposits. The callose deposits found in suspension culture cells are restricted to regions where unusual outgrowths or protuberances are formed on the cell surfaces, specifically regions that are the sites of new cell wall formation.Callose formation has been shown to be regulated by intracellular calcium levels.

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Faculty Opinions recommendation of Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.735406304.793563599 ◽

2019 ◽

Author(s):

Federica Brandizzi

Keyword(s):

Cell Wall ◽

Golgi Membranes ◽

Wall Structures ◽

Distinct Cell

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Evaluating Metagenomic Analysis for Pathogen Transmission in Healthcare Settings

Infection Control and Hospital Epidemiology ◽

10.1017/ice.2020.768 ◽

2020 ◽

Vol 41 (S1) ◽

pp. s224-s224

Author(s):

Curt Hewitt ◽

Katharina Weber ◽

Danielle LeSassier ◽

Anthony Kappell ◽

Kathleen Schulte ◽

...

Keyword(s):

Antibiotic Resistance ◽

Dna Analysis ◽

Pathogen Transmission ◽

Species Level ◽

Metagenomic Analysis ◽

Skin Microbiome ◽

Limited Information ◽

Culture Methods ◽

Data Set ◽

Skin Contact

Background: The prevalence of healthcare-acquired infections (HAIs) and rising levels of antimicrobial resistance place a significant burden on modern healthcare systems. Cultures are typically used to track HAIs; however, culture methods provide limited information and are not applicable to all pathogens. Next-generation sequencing (NGS) can detect and characterize pathogens present within a sample, but few research studies have explored how NGS could be used to detect pathogen transmission events under HAI-relevant scenarios. The objective of this CDC-funded project was to evaluate and correlate sequencing approaches for pathogen transmission with standard culture-based analysis. Methods: We modeled pathogen transfer via hand contact using synthetic skin. These skin coupons were seeded with a community of commensal organisms to mimic the human skin microbiome. Pathogens were added at physiologically relevant high or low levels prior to skin-to-skin contact. The ESKAPE pathogens: E. faecium, S. aureus, K. pneumoniae, A. baumannii, P. aeruginosa, and Enterobacter spp plus C. difficile were employed because they are the most common antibiotic resistant HAIs. Pathogen transfer between skin coupons was measured following direct skin contact and fomite surface transmission. The effects of handwashing or fomite decontamination were also evaluated. Transferred pathogens were enumerated via culture to establish a robust data set against which DNA and RNA sequence analyses of the same samples could be compared. These data also provide a quantitative assessment of individual ESKAPE+C pathogen transfer rates in skin contact scenarios. Results: Metagenomic and metatranscriptomic analysis using custom analysis pipelines and reference databases successfully identified the commensal and pathogenic organisms present in each sample at the species level. This analysis also identified antibiotic resistance genes and plasmids. Metatranscriptomic analysis permitted not only gene identification but also confirmation of gene expression, a critical factor in the evaluation of antibiotic resistance. DNA analysis does not require cell viability, a key differentiator between sequencing and culturing reflected in simulated handwashing data. Sensitivity remains a key limitation of metagenomic analysis, as shown by the poor species identification and gene content characterization of pathogens present at low abundance within the simulated microbial community. Species level identification typically failed as ratios fell below 1:1,000 pathogen CFU:total community CFU. Conclusions: These findings demonstrate the strengths and weaknesses of NGS for molecular epidemiology. The data sets produced for this study are publicly available so they can be employed for future metagenomic benchmarking studies.Funding: NoneDisclosures: None

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Retention of Proanthocyanidin in Wine-like Solution Is Conferred by a Dynamic Interaction between Soluble and Insoluble Grape Cell Wall Components

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.6b02900 ◽

2016 ◽

Vol 64 (44) ◽

pp. 8406-8419 ◽

Cited By ~ 14

Author(s):

Keren A. Bindon ◽

Sijing Li ◽

Stella Kassara ◽

Paul A. Smith

Keyword(s):

Cell Wall ◽

Dynamic Interaction ◽

Cell Wall Components ◽

Grape Cell ◽

Wall Components

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Biological Function(s) and Application (s) of Pectin and Pectin Degrading Enzymes

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2611 ◽

2018 ◽

Vol 15 (1) ◽

pp. 87-100 ◽

Cited By ~ 2

Author(s):

Puja Chandrayan

Keyword(s):

Cell Wall ◽

Structure Function ◽

Plant Cell ◽

Plant Cell Wall ◽

Plant Defence ◽

Glycoside Hydrolases ◽

Limited Information ◽

Thermostable Enzymes ◽

Network Cell ◽

Degrading Enzymes

Pectin is an integral part of plant cell wall and since centuries pectin extracted from plants is widely used in food and fruit juice processing. Moreover, in last half century, the applications have also invaded into many bio-processing applications such as pharmaceutical, bioenergy, textile, paper and tea processing. In these growing industries, the use of pectinases has grown with a significant amount i.e. approximately 10 % of total global enzyme market comes from pectinases. Herein comprehensive analyses of information related to structure and function of pectin in plant cell wall as well as structural classes of pectins have been discussed. The major function of pectin is in cementing the cellulose and hemicelluloses network, cell-cell adhesion and plant defence. Keeping the wide use of pectin in food industry and growing need of environment friendly technology for pectin extraction has accelerated the demand of pectin degrading enzymes (PDEs). PDEs are from three enzyme classes: carbohydrate esterases from CE8 and CE12 family, glycoside hydrolases from GH28 family and lyases from PL1, 2, 3, 9 and 10. We have reviewed the literature related to abundance and structure-function of these abovementioned enzymes from bacteria. From the current available literature, we found very limited information is present about thermostable PDEs. Hence, in future it could be a topic of study to gain the insight about structure-function of enzymes together with the expanded role of thermostable enzymes in development of bioprocesses based on these enzymes.

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AtSYP71 Is Important for Plant Cell Wall Biosynthesis and Stress Adaptation.

10.21203/rs.3.rs-1189370/v1 ◽

2021 ◽

Author(s):

Xiaoyue Kou ◽

Hailong Zhang ◽

Xiaonan Zhao ◽

Mingjing Wang ◽

Guochen Qin ◽

...

Keyword(s):

Cell Wall ◽

Early Development ◽

Plant Development ◽

Plant Cell Wall ◽

Cell Plate ◽

Development Stage ◽

Cell Wall Biosynthesis ◽

Knockout Mutant ◽

Wall Structures ◽

Confocal Images

Abstract Background: SYP71, the plant-specific Qc-SNARE protein, is reported to regulate vesicle trafficking. SYP71 is localized on the ER, endosome, plasma membrane and cell plate, suggesting its multiple functions. Lotus SYP71 is essential for symbiotic nitrogen fixation in nodules. AtSYP71, GmSYP71 and OsSYP71 are implicated in plant resistance to pathogenesis. To date, SYP71 regulatory role on plant development remain unclear.Results: AtSYP71-knockout mutant atsyp71-4 was lethal at early development stage. Early development of AtSYP71-knockdown mutant atsyp71-2 was delayed, and stress response was also affected. Confocal images revealed that protein secretion was blocked in atsyp71-2. Transcriptomic analysis indicated that metabolism, response to environmental stimuli pathways and apoplast components were influenced in atsyp71-2. Moreover, the contents of lignin, cellulose and flavonoids as well as cell wall structures were also altered.Conclusion: Our findings suggested that AtSYP71 is essential for plant development. AtSYP71 probably regulates plant development, metabolism and environmental adaptation by affecting cell wall homeostasis via mediating secretion of materials and regulators required for cell wall biosynthesis and dynamics.

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