scholarly journals Graphene-Like Layers from Carbon Black: In Vivo Toxicity Assessment

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1472
Author(s):  
Marta d’Amora ◽  
Michela Alfe ◽  
Valentina Gargiulo ◽  
Silvia Giordani

Graphene-like (GL) layers, a new graphene-related material (GRM), possess peculiar chemical, colloidal, optical and transport properties. Considering the very recent promising application of GL layers in biomedical and bioelectronic fields, it is of utmost importance to investigate the toxicological profile of these nanomaterials. This study represents an important first report of a complete in vivo toxicity assessment of GL layers on embryonic zebrafish (Danio rerio). Our results show that GL layers do not lead to any perturbations in the different biological parameters evaluated, indicating their good biocompatibility on a vertebrate model. The new insight into the biosafety of GL layers will expand their applications in nanomedicine.

2019 ◽  
Vol 31 (1) ◽  
pp. 185-195 ◽  
Author(s):  
Remya Rajan Renuka ◽  
Radhika Rajasree Santha Ravindranath ◽  
Vasantharaja Raguraman ◽  
Suman Thodhal Yoganandham ◽  
Govindaraju Kasivelu ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3403
Author(s):  
Nurshafika Mohd Sakeh ◽  
Nurliyana Najwa Md Razip ◽  
Farah Idayu Mohd Ma’in ◽  
Mohammad Nazri Abdul Bahari ◽  
Naimah Latif ◽  
...  

Excessive production of melanin implicates hyperpigmentation disorders. Flavokawain A (FLA) and flavokawain B (FLB) have been reported with anti-melanogenic activity, but their melanogenic inhibition and toxicity effects on the vertebrate model of zebrafish are still unknown. In the present study, cytotoxic as well as melanogenic effects of FLA and FLB on cellular melanin content and tyrosinase activity were evaluated in α-MSH-induced B16/F10 cells. Master regulator of microphthalmia-associated transcription factor (Mitf) and the other downstream melanogenic-related genes were verified via quantitative real time PCR (qPCR). Toxicity assessment and melanogenesis inhibition on zebrafish model was further observed. FLA and FLB significantly reduced the specific cellular melanin content by 4.3-fold and 9.6-fold decrement, respectively in α-MSH-induced B16/F10 cells. Concomitantly, FLA significantly reduced the specific cellular tyrosinase activity by 7-fold whilst FLB by 9-fold. The decrement of melanin production and tyrosinase activity were correlated with the mRNA suppression of Mitf which in turn down-regulate Tyr, Trp-1 and Trp-2. FLA and FLB exhibited non-toxic effects on the zebrafish model at 25 and 6.25 µM, respectively. Further experiments on the zebrafish model demonstrated successful phenotype-based depigmenting activity of FLA and FLB under induced melanogenesis. To sum up, our findings provide an important first key step for both of the chalcone derivatives to be further studied and developed as potent depigmenting agents.


2010 ◽  
Vol 44 (2) ◽  
pp. 439-448 ◽  
Author(s):  
Daniel Stalter ◽  
Axel Magdeburg ◽  
Mirco Weil ◽  
Thomas Knacker ◽  
Jörg Oehlmann

2017 ◽  
Vol 35 (2) ◽  
pp. 341-350 ◽  
Author(s):  
Xiaohu Luo ◽  
Ke Li ◽  
Jiali Xing ◽  
Lijun Qi ◽  
Ming Yang ◽  
...  

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