scholarly journals Molecular Characterization of the env Gene of Bovine Leukemia Virus in Cattle from Pakistan with NGS-Based Evidence of Virus Heterogeneity

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 910
Author(s):  
Marzena Rola-Łuszczak ◽  
Ali Sakhawat ◽  
Aneta Pluta ◽  
Anna Ryło ◽  
Arkadiusz Bomba ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Research Effort ◽  
Phylogenetic Analyses ◽  
Leukemia Virus ◽  
Potential Effect ◽  
Amino Acid Substitutions ◽  
Serological Detection ◽  
Genotype G1

Characterization of the global genetic diversity of the bovine leukemia virus (BLV) is an ongoing international research effort. Up to now BLV sequences have been classified into eleven distinct genotypes. Although BLV genotyping and molecular analysis of field isolates were reported in many countries, there is no report describing BLV genotypes present in cattle from Pakistan. In this study we examined 27 env gene sequences from BLV-infected cattle coming from four farms located in Khyber Pakhtunkwa, Gilgit Baltisan and Punjab provinces. Phylogenetic analyses revealed the classification of Pakistani sequences into genotypes G1 and G6. The alignment with the FLK-BLV sequence revealed the presence of 45 mutations, namely, seven in genotype G1 and 33 in genotype G6. Five mutations were found in both, G1 and G6 genotypes. Twelve amino acid substitutions were found in the analyzed sequences, of which only one P264S was specific for sequences from Pakistan. Furthermore, a certain degree of nucleotide heterogeneity was identified by NGS. These results highlight the need for further study on the importance of genetic variability of BLV, especially in the context of its pathogenicity and potential effect on serological detection.

scholarly journals The Immunophenotypic Characterization of Bovine Lymphomas

1997 ◽  
Vol 34 (3) ◽  
pp. 222-225 ◽  
Author(s):  
W. Vernau ◽  
R. M. Jacobs ◽  
V. E. O. Valli ◽  
J. L. Heeney
Keyword(s):  
B Cell ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Cell Phenotype ◽  
Prospective Analysis ◽  
Cell Type ◽  
Working Formulation ◽  
Morphologic Classification

A prospective analysis of 38 cases of bovine lymphoma was performed. Thirty-five of the lymphomas were considered enzootic because of serologic or molecular biologic association with the bovine leukemia virus (BLV). The three remaining cases were in cattle <1 year of age and were not associated with BLV and hence were considered sporadic lymphomas. Utilizing the National Cancer Institute Working Formulation for the morphologic classification of human non-Hodgkin's lymphoma, 54.3% (19/35) of the enzootic lymphomas were classified as the diffuse large cleaved cell type. The cells of the 35 enzootic lymphomas expressed a consistent immunophenotype represented by MHCII+, γ-heavy chain+, and λ-light chain+; this constellation of markers indicated a mature B-cell phenotype. The three sporadic lymphomas failed to express MHCII antigen despite the presence of other B-cell antigens. There was no correlation between morphologic cell type and immunophenotype in these bovine lymphomas.

scholarly journals Serological detection of bovine leukemia virus in slaughterhouse workers from San Nicols de los Garza, Nuevo Len, Mxico

2013 ◽  
Vol 7 (24) ◽  
pp. 3042-3048
Author(s):  
E Zamora Avila Diana ◽  
Zapata Benavides Pablo ◽  
Cedillo Rosales Sibilina ◽  
Avalos Ramiacute rez Ramiro ◽  
J Zarate Ramos Juan ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Serological Detection

Antigenic variants of bovine leukemia virus (BLV) are defined by amino acid substitutions in the NH2 part of the envelope glycoprotein gp51

Virology ◽  
1989 ◽  
Vol 169 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Daniel Portetelle ◽  
Dominique Couez ◽  
Claudine Bruck ◽  
Richard Kettmann ◽  
Marc Mammerickx ◽  
...  
Keyword(s):  
Amino Acid ◽  
Envelope Glycoprotein ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Amino Acid Substitutions

Molecular detection and characterization of genotype 1 bovine leukemia virus from beef cattle in the traditional sector in Zambia

2019 ◽  
Vol 164 (10) ◽  
pp. 2531-2536 ◽  
Author(s):  
Mundia M. Phiri ◽  
Evans Kaimoyo ◽  
Katendi Changula ◽  
Isaac Silwamba ◽  
Herman M. Chambaro ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Bovine Leukemia Virus ◽  
Molecular Detection ◽  
Leukemia Virus ◽  
Genotype 1 ◽  
Traditional Sector

Biochemical Characterization of Bovine Leukemia Virus

2015 ◽  
pp. 363-365
Author(s):  
D. Portetelle ◽  
R. Kettmann ◽  
M. Mammerickx ◽  
Y. Cleuter ◽  
M. Galoux ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Biochemical Characterization ◽  
Leukemia Virus

Detection and molecular characterization of bovine leukemia virus in Philippine cattle

2014 ◽  
Vol 160 (1) ◽  
pp. 285-296 ◽  
Author(s):  
Meripet Polat ◽  
Ayumu Ohno ◽  
Shin-nosuke Takeshima ◽  
Jiyun Kim ◽  
Mari Kikuya ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus

scholarly journals First Molecular Characterization of Bovine Leukemia Virus Infections in the Caribbean

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0168379 ◽  
Author(s):  
Yi Yang ◽  
Patrick John Kelly ◽  
Jianfa Bai ◽  
Rong Zhang ◽  
Chengming Wang
Keyword(s):  
Molecular Characterization ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Infections ◽  
The Caribbean

scholarly journals Detection and molecular characterization of bovine leukemia virus in beef cattle presented for slaughter in Egypt

2020 ◽  
Vol 82 (11) ◽  
pp. 1676-1684
Author(s):  
Samy METWALLY ◽  
Rania HAMADA ◽  
Alsagher O. ALI ◽  
Hassan Y.A.H. MAHMOUD ◽  
Nabil M. BAKER ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Molecular Characterization ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus

scholarly journals MASKED GENOTYPE 6 OF BOVINE LEUKEMIA VIRUS IS FOUND IN COLOMBIAN CATTLE

2019 ◽  
Author(s):  
Adriana Patricia Corredor-Figueroa ◽  
Nury Nathalia Olaya-Galán ◽  
Sandra Patricia Salas ◽  
Juan Sebastián Quintero ◽  
Álvaro Fajardo ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Bovine Leukemia Virus ◽  
Phylogenetic Analyses ◽  
Leukemia Virus ◽  
Gene Analysis ◽  
Economic Losses ◽  
Envelope Gene ◽  
Genotype 1 ◽  
Global Epidemiology ◽  
Bovine Leukosis

Abstract Background Viral diseases such as bovine leukosis are a major cause of health problems associated with economic losses in the livestock industry worldwide. The prevalence of bovine leukosis ranges between 4% and 90%, and this disease is considered endemic. The etiological agent is bovine leukemia virus (BLV). Ten BLV genotypes have been reported based on analysis of complete or partial envelope gene sequences, of which genotype 1 is the most prevalent. However, the genetic variability of BLV variants circulating in Colombia remains unexplored. Therefore, in the present study, we analyzed blood samples collected from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. Results A total of 62% of the cows present at 92% of the farms were BLV-positive. Genotype 1 was exclusively detected by env and gag gene segments when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. Conclusions This report is the first molecular and epidemiological study of BLV conducted in Colombia and contributes to the global epidemiology of the virus; it also reinforces the great impact of BLV on the country’s livestock and thus is a useful resource for farmers and government entities.

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