Engineered EVs for Oxidative Stress Protection

Extracellular vesicles (EVs) are increasingly studied as vectors for drug delivery because they can transfer a variety of molecules across biological barriers. SerpinB3 is a serine protease inhibitor that has shown a protective anti-apoptotic function in a variety of stressful conditions. The aim of this study was to evaluate protection from oxidative stress-induced damage, using extracellular vesicles that overexpress SerpinB3 (EVs-SB3) in order to enhance the effect of extracellular vesicles on cellular homeostasis. EVs-SB3s were obtained from HepG2 cells engineered to overexpress SerpinB3 and they revealed significant proteomic changes, mostly characterized by a reduced expression of other proteins compared with EVs from non-engineered cells. These EV preparations showed a significantly higher protection from H2O2 induced oxidative stress in both the hepatoma cell line and in primary cardiomyocytes, compared to cells treated with naïve EVs or SerpinB3 alone, used at the same concentration. In conclusion, the induction of SerpinB3 transgene expression results in the secretion of EVs enriched with the protein product that exhibits enhanced cytoprotective activity, compared with naïve EVs or the nude SerpinB3 protein.

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Phyllanthin of Standardized Phyllanthus amarus Extract Attenuates Liver Oxidative Stress in Mice and Exerts Cytoprotective Activity on Human Hepatoma Cell Line

Journal of Clinical and Experimental Hepatology ◽

10.1016/s0973-6883(11)60123-0 ◽

2011 ◽

Vol 1 (2) ◽

pp. 57-67 ◽

Cited By ~ 13

Author(s):

Rajesh Krithika ◽

Ramtej J Verma ◽

Pranav S Shrivastav ◽

Lonchin Suguna

Keyword(s):

Oxidative Stress ◽

Cell Line ◽

Hepatoma Cell ◽

Hepatoma Cell Line ◽

Phyllanthus Amarus ◽

Human Hepatoma ◽

Human Hepatoma Cell ◽

Human Hepatoma Cell Line ◽

Cytoprotective Activity

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Caveolin-1 promotes radioresistance in rhabdomyosarcoma through increased oxidative stress protection and DNA repair

Cancer Letters ◽

10.1016/j.canlet.2021.02.005 ◽

2021 ◽

Vol 505 ◽

pp. 1-12

Author(s):

Silvia Codenotti ◽

Francesco Marampon ◽

Luca Triggiani ◽

Marco Lorenzo Bonù ◽

Stefano Maria Magrini ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Repair ◽

Caveolin 1 ◽

Stress Protection ◽

Oxidative Stress Protection

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TGF-beta-induced early gene-1 overexpression promotes oxidative stress protection and actin cytoskeleton rearrangement in human skin fibroblasts

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2016.02.009 ◽

2016 ◽

Vol 1860 (6) ◽

pp. 1071-1078 ◽

Cited By ~ 10

Author(s):

Chloe Leduc ◽

Lauren Sobilo ◽

Hechmi Toumi ◽

Philippe Mondon ◽

Eric Lespessailles ◽

...

Keyword(s):

Oxidative Stress ◽

Actin Cytoskeleton ◽

Human Skin ◽

Skin Fibroblasts ◽

Early Gene ◽

Tgf Beta ◽

Human Skin Fibroblasts ◽

Stress Protection ◽

Oxidative Stress Protection ◽

Cytoskeleton Rearrangement

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Increased Cytotoxicity of Carbon Tetrachloride in a Human Hepatoma Cell Line Overexpressing Cytochrome P450 2E1

Journal of International Medical Research ◽

10.1177/147323000203000406 ◽

2002 ◽

Vol 30 (4) ◽

pp. 400-405 ◽

Cited By ~ 15

Author(s):

S Takahashi ◽

T Takahashi ◽

S Mizobuchi ◽

M Matsumi ◽

K Morita ◽

...

Keyword(s):

Oxidative Stress ◽

Cytochrome P450 ◽

Carbon Tetrachloride ◽

Cell Line ◽

Hepatoma Cell ◽

Mrna Level ◽

Hepatoma Cell Line ◽

Cytochrome P450 2E1 ◽

Hsp70 Mrna ◽

Potential Marker

Cytotoxic free radicals generated during the metabolism of carbon tetrachloride by cytochrome P450 2E1 (CYP2E1) are thought to cause hepatotoxicity. Here, the cytotoxic effects of carbon tetrachloride in a liver cell line expressing CYP2E1 (HLE/2E1) are compared with those in the mother cell line (HLE). The effects of carbon tetrachloride on the gene expression of HSP70, a potential marker of oxidative stress, were also examined. The viability of HLE/2E1 cells after exposure to carbon tetrachloride was significantly decreased compared with that of HLE cells. Northern blot analysis revealed that the HSP70 mRNA level was significantly increased after carbon tetrachloride treatment in both cell lines, while the magnitude of its increase was much greater in HLE/2E1 cells than in HLE cells. These results suggest that the oxidative stress induced by CYP2E1 plays an important role in the increase in cytotoxicity of carbon tetrachloride in CYP2E1-overexpressing cells.

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