scholarly journals Biohydrogen Production from Food Waste: Influence of the Inoculum-To-Substrate Ratio

2018 ◽  
Vol 10 (12) ◽  
pp. 4506 ◽  
Author(s):  
Giovanna Cappai ◽  
Giorgia De Gioannis ◽  
Aldo Muntoni ◽  
Daniela Spiga ◽  
Maria Rosaria Boni ◽  
...  
Keyword(s):  
Food Waste ◽  
H2 Production ◽  
Fermentation Products ◽  
Fermentative Hydrogen Production ◽  
Batch Tests ◽  
Optimal Value ◽  
Substrate Ratio ◽  
Fermentative Hydrogen ◽  
H2 Yield ◽  
Maximum Production Rate

In this study, the influence of the inoculum-to-substrate ratio (ISR) on dark fermentative hydrogen production from food waste (FW) was evaluated. ISR values ranging from 0.05 to 0.25 g VSinoculum/g VSsubstrate were investigated by performing batch tests at T = 39 °C and pH = 6.5, the latter being the optimal value identified based on a previous study. The ISR was found to affect the fermentation process, clearly showing that an adequate ISR is essential in order to optimise the process kinetics and the H2 yield. An ISR of 0.14 proved to optimum, leading to a maximum H2 yield of 88.8 L H2/kg VSFW and a maximum production rate of 10.8 L H2/kg VSFW∙h. The analysis of the fermentation products indicated that the observed highest H2 production mostly derived from the typical acetate/butyrate-type fermentation.

Measurement of H2 consumption and its role in continuous fermentative hydrogen production

2008 ◽  
Vol 57 (5) ◽  
pp. 681-685 ◽  
Author(s):  
J. T. Kraemer ◽  
D. M. Bagley
Keyword(s):  
Continuous Flow ◽  
H2 Production ◽  
Continuous Reactor ◽  
Yield Increase ◽  
Fermentative Hydrogen Production ◽  
Consumption Rates ◽  
Fermentative Hydrogen ◽  
H2 Yield ◽  
First Time

To maximise the yield from fermentative H2 production, H2 consumption must be minimised. This work demonstrated for the first time that H2 consumption exists in an established continuous-flow biohydrogen system. The rate of H2 consumption was found to be related to the concentration of CO2, with H2 consumption inhibited at both low and high CO2. N2 sparging of the continuous reactor at 31 mL/min/L-liquid increased the H2 yield from 1.31 to 1.87 mol H2/mol glucose, but did not significantly change the in-situ rate of H2 consumption (0.07–0.09 mM/h). Assuming sparging completely inhibited H2 consumption, it could only account for 2–11% of the H2 yield increase during sparging, based on H2 consumption rates measured in the reactor and in vials. Therefore, H2 consumption may be of minor concern for continuous biohydrogen systems.

Fermentative Hydrogen Production - Process Design and Bioreactors

2012 ◽  
Vol 33 (4) ◽  
pp. 585-594 ◽  
Author(s):  
Małgorzata Waligórska
Keyword(s):  
Production Rate ◽  
Alternative Energy ◽  
Fossil Fuels ◽  
H2 Production ◽  
Factors Affecting ◽  
Fermentative Hydrogen Production ◽  
Production Factors ◽  
Fermentative Hydrogen ◽  
And Performance ◽  
H2 Yield

Substitution of fossil fuels with alternative energy carriers has become necessary due to climate change and fossil fuel shortages. Fermentation as a way of producing biohydrogen, an attractive and environmentally friendly future energy carrier, has captured received increasing attention in recent years because of its high H2 production rate and a variety of readily available waste substrates used in the process. This paper discusses the state-of-the-art of fermentative biohydrogen production, factors affecting this process, as well as various bioreactor configurations and performance parameters, including H2 yield and H2 production rate.

Fermentative Hydrogen Production From Food Waste Without Inocula

2008 ◽  
Author(s):  
S. Shimizu ◽  
A. Fujisawa ◽  
O. Mizuno ◽  
T. Kameda ◽  
T. Yoshioka ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Fermentative Hydrogen Production ◽  
Fermentative Hydrogen

scholarly journals Susceptibility of the Formate Hydrogenlyase Reaction to the Protonophore CCCP Depends on the Total Hydrogenase Composition

Inorganics ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 38
Author(s):  
Janik Telleria Marloth ◽  
Constanze Pinske
Keyword(s):  
Complex I ◽  
H2 Production ◽  
Proton Pumping ◽  
Fermentative Hydrogen Production ◽  
Iron Sulfur ◽  
Formate Hydrogenlyase ◽  
Respiratory Complex I ◽  
Fermentative Hydrogen ◽  
Pumping Activity ◽  
Higher Sensitivity

Fermentative hydrogen production by enterobacteria derives from the activity of the formate hydrogenlyase (FHL) complex, which couples formate oxidation to H2 production. The molybdenum-containing formate dehydrogenase and type-4 [NiFe]-hydrogenase together with three iron-sulfur proteins form the soluble domain, which is attached to the membrane by two integral membrane subunits. The FHL complex is phylogenetically related to respiratory complex I, and it is suspected that it has a role in energy conservation similar to the proton-pumping activity of complex I. We monitored the H2-producing activity of FHL in the presence of different concentrations of the protonophore CCCP. We found an inhibition with an apparent EC50 of 31 µM CCCP in the presence of glucose, a higher tolerance towards CCCP when only the oxidizing hydrogenase Hyd-1 was present, but a higher sensitivity when only Hyd-2 was present. The presence of 200 mM monovalent cations reduced the FHL activity by more than 20%. The Na+/H+ antiporter inhibitor 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) combined with CCCP completely inhibited H2 production. These results indicate a coupling not only between Na+ transport activity and H2 production activity, but also between the FHL reaction, proton import and cation export.

Factors Influenced Continuous Dark Fermentative Hydrogen-Producing Reactor

2011 ◽  
Vol 183-185 ◽  
pp. 457-461 ◽  
Author(s):  
Ying Yue ◽  
Lu Si Zhang ◽  
Jie Ding
Keyword(s):  
Hydrogen Production ◽  
Environmental Change ◽  
Production Technology ◽  
Commercial Application ◽  
Reactor Operation ◽  
Fermentative Hydrogen Production ◽  
Factors Influencing ◽  
Optimal Value ◽  
Fermentative Hydrogen

Continuous dark fermentative hydrogen production technology is suitable for commercial application. This review summarized several main basic factors influencing the operation of the reactor, followed by some suggestions and outlooks. The factors included temperature, pH, HRT and COD were briefly introduced and discussed. This review demonstrated that the optimal value of a given factor under different conditions was great different. This indicates that reactor operation is affected by many factors and sensitive to environmental change. To make the technology more feasible into practice, deeply understanding about the characteristics and rules of the operation is necessary. Thus more researches in this respect are recommended.

Research Progress of Food Waste Fermentation for Bio-Hydrogen Production

2012 ◽  
Vol 550-553 ◽  
pp. 569-573
Author(s):  
Xiao Fang Yue ◽  
Hong Yuan Sun ◽  
Xu Xin Zhao ◽  
Li Qing Zhao
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Waste Treatment ◽  
Clean Energy ◽  
Hydrogen Gas ◽  
Research Progress ◽  
Fermentative Hydrogen Production ◽  
Production Of Hydrogen ◽  
Fermentative Hydrogen ◽  
Clean Energy Source

Hydrogen is a valuable gas as a clean energy source and as feedstock for some industries. Therefore, demand on hydrogen production has increased considerably in recent years. Food waste is an important part of urban living garbage,which is full of organic matter and easy to be degraded. So, biological production of hydrogen gas from food waste fermentation has significant advantages for providing inexpensive and clean energy generation to help meet the needs of carbon emission reduction with simultaneous waste treatment. This article reviews the following aspects: mechanism of fermentative hydrogen production by bacteria, and factors influencing fermentative bio-hydrogen production. In addition,the challenges and prospects of bio- hydrogen production are also reviewed.

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