The Incidence and Genetic Diversity of Apple Mosaic Virus (ApMV) and Prune Dwarf Virus (PDV) in Prunus Species in Australia

In the fruit belt of the Niagara Peninsula, wild Prunus avium and P. serotina are common along the Niagara Escarpment, the larger streams, and the Niagara River. P. virginiana is somewhat more widespread than the former species and P. pensylvanica is very rare. P. nigra and P. americana are limited in distribution. The necrotic ringspot virus (NRSV) was detected in trees of P. avium, P. serotina, and P. virginiana, whereas the prune dwarf virus (PDV) (sour cherry yellows) was found only in trees of P. avium and P. serotina. Because of the limited incidence and distribution of virus-infected trees, however, and because the bloom periods of the wild species rarely coincide with sour cherry, peach, or plum, wild Prunus species are considered relatively unimportant as potential reservoirs of virus for infection of commercial orchards.

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Detecção e caracterização molecular dos genes da proteína capsidial de ilarvírus e ampelovírus que infectam fruteiras temperadas

Ciência Rural ◽

10.1590/s0103-84782011000100002 ◽

2011 ◽

Vol 41 (1) ◽

pp. 5-9 ◽

Cited By ~ 3

Author(s):

Thor Vinícius Martins Fajardo ◽

Osmar Nickel ◽

Marcelo Eiras

Keyword(s):

Western Blot ◽

Mosaic Virus ◽

Dwarf Virus ◽

Rt Pcr ◽

Prune Dwarf Virus

Dentre os principais patógenos que incidem em fruteiras temperadas, destacam-se o Prune dwarf virus (PDV), o Apple mosaic virus (ApMV) e o Grapevine leafroll-associated virus 1 (GLRaV-1). Neste trabalho foram realizadas a detecção e a caracterização molecular dos genes da proteína capsidial de isolados destas três espécies virais. RNAs totais foram extraídos de amostras de folhas de pessegueiros, macieiras e videiras e, nas reações de RT-PCR, foram utilizados oligonucleotídeos específicos para cada espécie viral. Os cDNAs amplificados foram clonados e sequenciados. Foram verificadas altas identidades entre as sequências de nucleotídeos dos genes da proteína capsidial dos isolados brasileiros de PDV, ApMV e GLRaV-1 e isolados de outros países, independente da origem geográfica e da hospedeira. O peso molecular da proteína capsidial destes vírus foi estimado por meio de Western blot em cerca de 24kDa (PDV), 26kDa (ApMV) e 39kDa (GLRaV-1).

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Vergleich verschiedener methoden zum nachweis des nekrotischen und chlorotischen ringflecken-virus der kirsche (Prunus necrotic ringspot virus, PNRV; prune dwarf virus, PDV) sowie des apfelmosaik-virus (apple mosaic virus, ApMV) in pflaumenbäumen

Zentralblatt für Mikrobiologie ◽

10.1016/s0232-4393(88)80005-2 ◽

1988 ◽

Vol 143 (1) ◽

pp. 25-37 ◽

Cited By ~ 3

Author(s):

M. Grüntzig ◽

E. Fuchs

Keyword(s):

Mosaic Virus ◽

Ringspot Virus ◽

Dwarf Virus ◽

Prune Dwarf Virus ◽

Prunus Necrotic Ringspot Virus

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Viruses of blackthorn and road-bordering trees of plum, myrobalan, sweet and sour cherries in the Czech Republic

Plant Protection Science ◽

10.17221/2351-pps ◽

2008 ◽

Vol 43 (No. 1) ◽

pp. 1-4 ◽

Cited By ~ 2

Author(s):

J. Polák

Keyword(s):

Czech Republic ◽

Mosaic Virus ◽

Sour Cherry ◽

Ringspot Virus ◽

Dwarf Virus ◽

Plum Pox Virus ◽

The Czech Republic ◽

Prune Dwarf Virus ◽

Prunus Necrotic Ringspot Virus ◽

Leafroll Virus

The distribution of Plum pox virus (PPV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic ringspot virus (ACLSV) and Apple mosaic virus (ApMV) in naturally growing shrubs of blackthorn and road-bordering trees of plum and myrobalan, and of PPV, PDV, PNRSV and Cherry leafroll virus (CLRV) in sweet and sour cherry trees were investigated. The most widely distributed viruses were PPV in plums (74% of the investigated trees were infected); PPV, PDV, and PNRSV in myrobalans (26%, 11% and 18%, respectively), PDV in blackthorns (27%), and PDV and PNRSV in cherries (25% and 22%). PPV was not detected in sweet and sour cherries. The incidence of ACLSV and ApMV was negligible in individually growing trees of the genus Prunus in the Czech Republic.

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