Faculty Opinions recommendation of New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation.

2001 ◽  
Author(s):  
Angel Nebreda
Keyword(s):  
Oocyte Maturation ◽  
Xenopus Oocyte ◽  
Meiosis I

scholarly journals Xenopus oocyte maturation does not require new cyclin synthesis.

1991 ◽  
Vol 114 (4) ◽  
pp. 767-772 ◽  
Author(s):  
J Minshull ◽  
A Murray ◽  
A Colman ◽  
T Hunt
Keyword(s):  
Protein Synthesis ◽  
Oocyte Maturation ◽  
Antisense Oligonucleotides ◽  
Xenopus Oocyte ◽  
Xenopus Oocytes ◽  
Cyclin A ◽  
Cyclin B1 ◽  
Meiotic Metaphase ◽  
Pass Through ◽  
Meiosis I

Progesterone induces fully grown, stage VI, Xenopus oocytes to pass through meiosis I and arrest in metaphase of meiosis II. Protein synthesis is required twice in this process: in order to activate maturation promoting factor (MPF) which induces meiosis I, and then again after the completion of meiosis I to reactivate MPF in order to induce meiosis II. We have used antisense oligonucleotides to destroy maternal stores of cyclin mRNAs, and demonstrate that new cyclin synthesis is not required for entry into either meiosis I or II. This finding is consistent with the demonstration that stage VI oocytes contain a store of B-type cyclin polypeptides (Kobayashi, H., J. Minshull, C. Ford, R. Golsteyn, R. Poon, and T. Hunt. 1991. J. Cell Biol. 114:755-765). Although approximately 70% of cyclin B2 is destroyed at first meiosis, the surviving fraction, together with a larger pool of surviving cyclin B1, must be sufficient to allow the reactivation of MPF and induce entry into second meiotic metaphase. Since stage VI oocytes do not contain any cyclin A, our results show that cyclin A is not required for meiosis in Xenopus. We discuss the possible nature of the proteins whose synthesis is required to induce meiosis I and II.

c-Mos and cyclin B/cdc2 connections during Xenopus oocyte maturation

2001 ◽  
Vol 93 (1-2) ◽  
pp. 15-25 ◽  
Author(s):  
Anna Castro ◽  
Marion Peter ◽  
Thierry Lorca ◽  
Elisabeth Mandart
Keyword(s):  
Oocyte Maturation ◽  
Xenopus Oocyte ◽  
Cyclin B

scholarly journals Regulation of Src kinase activity during Xenopus oocyte maturation

2005 ◽  
Vol 278 (2) ◽  
pp. 289-300 ◽  
Author(s):  
Alexander Tokmakov ◽  
Tetsushi Iwasaki ◽  
Shuji Itakura ◽  
Ken-Ichi Sato ◽  
Mikako Shirouzu ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Xenopus Oocyte ◽  
Kinase Activity ◽  
Src Kinase

In vitro facilitation of Xenopus oocyte maturation by subthreshold doses of progesterone

1977 ◽  
Vol 59 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Jean Marot ◽  
Robert Bellé ◽  
René Ozon
Keyword(s):  
Oocyte Maturation ◽  
Xenopus Oocyte

scholarly journals Reorganization of actin filaments by ADF/cofilin is involved in formation of microtubule structures during Xenopus oocyte maturation

2015 ◽  
Vol 26 (24) ◽  
pp. 4387-4400 ◽  
Author(s):  
Yuka Yamagishi ◽  
Hiroshi Abe
Keyword(s):  
Oocyte Maturation ◽  
Actin Filament ◽  
Actin Filaments ◽  
Xenopus Oocyte ◽  
Germinal Vesicle ◽  
Meiotic Spindle ◽  
Base Region ◽  
Microtubule Organizing Center ◽  
Cytoplasmic Actin ◽  
Organizing Center

We examined the reorganization of actin filaments and microtubules during Xenopus oocyte maturation. Surrounding the germinal vesicle (GV) in immature oocytes, the cytoplasmic actin filaments reorganized to accumulate beneath the vegetal side of the GV, where the microtubule-organizing center and transient microtubule array (MTOC-TMA) assembled, just before GV breakdown (GVBD). Immediately after GVBD, both Xenopus ADF/cofilin (XAC) and its phosphatase Slingshot (XSSH) accumulated into the nuclei and intranuclear actin filaments disassembled from the vegetal side with the shrinkage of the GV. As the MTOC-TMA developed well, cytoplasmic actin filaments were retained at the MTOC-TMA base region. Suppression of XAC dephosphorylation by anti-XSSH antibody injection inhibited both actin filament reorganization and proper formation and localization of both the MTOC-TMA and meiotic spindles. Stabilization of actin filaments by phalloidin also inhibited formation of the MTOC-TMA and disassembly of intranuclear actin filaments without affecting nuclear shrinkage. Nocodazole also caused the MTOC-TMA and the cytoplasmic actin filaments at its base region to disappear, which further impeded disassembly of intranuclear actin filaments from the vegetal side. XAC appears to reorganize cytoplasmic actin filaments required for precise assembly of the MTOC and, together with the MTOC-TMA, regulate the intranuclear actin filament disassembly essential for meiotic spindle formation.

The mechanism for increased protein synthesis during Xenopus oocyte maturation

1982 ◽  
Vol 89 (1) ◽  
pp. 159-167 ◽  
Author(s):  
Joel D. Richter ◽  
William J. Wasserman ◽  
L.Dennis Smith
Keyword(s):  
Protein Synthesis ◽  
Oocyte Maturation ◽  
Xenopus Oocyte
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