Faculty Opinions recommendation of Rational design of green fluorescent protein mutants as biosensor for bacterial endotoxin.

2002 ◽  
Author(s):  
John Shanklin
Keyword(s):  
Green Fluorescent Protein ◽  
Rational Design ◽  
Fluorescent Protein ◽  
Bacterial Endotoxin ◽  
Protein Mutants ◽  
Green Fluorescent

scholarly journals Photophysics and optical switching in green fluorescent protein mutants

2000 ◽  
Vol 97 (7) ◽  
pp. 2974-2978 ◽  
Author(s):  
T. M. H. Creemers ◽  
A. J. Lock ◽  
V. Subramaniam ◽  
T. M. Jovin ◽  
S. Volker
Keyword(s):  
Green Fluorescent Protein ◽  
Optical Switching ◽  
Fluorescent Protein ◽  
Protein Mutants ◽  
Green Fluorescent

Computational study of the absorption spectra of green fluorescent protein mutants

Biopolymers ◽  
2007 ◽  
Vol 85 (3) ◽  
pp. 253-263 ◽  
Author(s):  
Soumya S. Patnaik ◽  
Steven Trohalaki ◽  
Rajesh R. Naik ◽  
Morley O. Stone ◽  
Ruth Pachter
Keyword(s):  
Green Fluorescent Protein ◽  
Absorption Spectra ◽  
Fluorescent Protein ◽  
Computational Study ◽  
Protein Mutants ◽  
Green Fluorescent

scholarly journals Regulated Expression of the Centrosomal Protein DdCP224 Affects Microtubule Dynamics and Reveals Mechanisms for the Control of Supernumerary Centrosome Number

2003 ◽  
Vol 14 (10) ◽  
pp. 4067-4074 ◽  
Author(s):  
Ralph Gräf ◽  
Ursula Euteneuer ◽  
Thi-Hieu Ho ◽  
Markus Rehberg
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Microtubule Organizing Centers ◽  
Protein Mutants ◽  
Bona Fide ◽  
Multinuclear Cells ◽  
Cycle Dependent ◽  
Green Fluorescent ◽  
First Time

The Dictyostelium XMAP215 family member DdCP224 is involved in centrosome duplication and cytokinesis and is concentrated at the centrosome and microtubule tips. Herein, we have created a DdCP224 promoter replacement mutant that allows both over- and underexpression. Overexpression led to supernumerary microtubule-organizing centers and, independently, an increase of the number of multinuclear cells. Electron microscopy demonstrated that supernumerary microtubule-organizing centers represented bona fide centrosomes. Live cell imaging of DdCP224-green fluorescent protein mutants also expressing green fluorescent protein-histone2B as a DNA label revealed that supernumerary centrosomes were also competent of cell cycle-dependent duplication. In contrast, underexpression of DdCP224 inhibited cell growth, reduced the number and length of astral microtubules, and caused nocodazole hypersensitivity. Moreover, microtubule regrowth after nocodazole removal was dependent on DdCP224. Underexpression also resulted in a striking disappearance of supernumerary centrosomes and multinuclear cells caused by previous overexpression. We show for the first time by live cell observation that the number of supernumerary centrosomes can be reduced either by centrosome fusion (coalescence) or by the formation of cytoplasts containing supernumerary centrosomes during cytokinesis.

Development and Characterization of Green Fluorescent Protein Mutants with Altered Lifetimes†

Biochemistry ◽  
2005 ◽  
Vol 44 (40) ◽  
pp. 13377-13384 ◽  
Author(s):  
Allan W. Scruggs ◽  
Carole L. Flores ◽  
Rebekka Wachter ◽  
Neal W. Woodbury
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Protein Mutants ◽  
Green Fluorescent
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