Faculty Opinions recommendation of Reversible site-selective labeling of membrane proteins in live cells.

2004 ◽  
Author(s):  
Vivian Tang
Keyword(s):  
Membrane Proteins ◽  
Live Cells ◽  
Selective Labeling ◽  
Site Selective

Reversible site-selective labeling of membrane proteins in live cells

2004 ◽  
Vol 22 (4) ◽  
pp. 440-444 ◽  
Author(s):  
Emmanuel G Guignet ◽  
Ruud Hovius ◽  
Horst Vogel
Keyword(s):  
Membrane Proteins ◽  
Live Cells ◽  
Selective Labeling ◽  
Site Selective

Abstract 1304: AbYlinkTM: A site-selective labeling method for preclinical imaging of therapeutic antibodies

2021 ◽  
Author(s):  
Viktoriia Postupalenko ◽  
Léo Marx ◽  
David Viertl ◽  
Natalia Gasilova ◽  
Mathilde Plantin ◽  
...  
Keyword(s):  
Therapeutic Antibodies ◽  
Selective Labeling ◽  
Preclinical Imaging ◽  
A Site ◽  
Site Selective ◽  
Labeling Method

scholarly journals Bioinspired Thiophosphorodichloridate Reagents for Chemoselective Histidine Bioconjugation

2018 ◽  
Author(s):  
Shang Jia ◽  
Christopher Chang
Keyword(s):  
Protein Function ◽  
Living Cells ◽  
Covalent Modification ◽  
Native Protein ◽  
Selective Labeling ◽  
Protein Residues ◽  
Mild Conditions ◽  
Starting Point ◽  
Histidine Phosphorylation ◽  
Site Selective

Site-selective bioconjugation to native protein residues is a powerful tool for protein functionalization, with cysteine and lysine side chains being the most common points for attachment owing to their high nucleophilicity. We now report a strategy for histidine modification using thiophosphorodichloridate reagents that mimic post-translational histidine phosphorylation, enabling fast and selective labeling of protein histidines under mild conditions where various payloads can be introduced via copper-assisted alkyne-azide cycloaddition (CuAAC) chemistry. We establish that these reagents are particularly effective at covalent modification of His-tags, which are common motifs to facilitate protein purification, as illustrated by selective attachment of polyarginine cargoes to enhance the uptake of proteins into living cells. This work provides a starting point for probing and enhancing protein function using histidine-directed chemistry.

scholarly journals Expanding Number and Brightness to Determine the Oligomer Size of Membrane Proteins in Live Cells as a Function of Concentration

2020 ◽  
Vol 118 (3) ◽  
pp. 246a
Author(s):  
Michael D. Paul ◽  
Yi Zuo ◽  
Randall Rainwater ◽  
Luo Gu ◽  
Kalina Hristova
Keyword(s):  
Membrane Proteins ◽  
Live Cells

scholarly journals Imaging GPCR internalization using near-infrared Nebraska red-based reagents

2020 ◽  
Vol 18 (13) ◽  
pp. 2459-2467
Author(s):  
Lauren Lesiak ◽  
Xinqi Zhou ◽  
Yuan Fang ◽  
Jia Zhao ◽  
Jon R. Beck ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Near Infrared ◽  
Selective Labeling

Near-infrared reagents for selective labeling of membrane proteins, no-wash imaging, and imaging GPCR internalization.

Selective labeling of functional groups on membrane proteins or glycoproteins using reactive biotin derivatives and 125I-streptavidin

1986 ◽  
Vol 136 (1) ◽  
pp. 80-85 ◽  
Author(s):  
Ehud Roffman ◽  
Leonid Meromsky ◽  
Haya Ben-Hur ◽  
Edward A. Bayer ◽  
Meir Wilchek
Keyword(s):  
Membrane Proteins ◽  
Functional Groups ◽  
Selective Labeling

Boronic Acids as Bioorthogonal Probes for Site‐Selective Labeling of Proteins

2018 ◽  
Vol 57 (40) ◽  
pp. 13028-13044 ◽  
Author(s):  
Burcin Akgun ◽  
Dennis G. Hall
Keyword(s):  
Boronic Acids ◽  
Selective Labeling ◽  
Site Selective

Peptide-Templated Acyl Transfer: A Chemical Method for the Labeling of Membrane Proteins on Live Cells

2014 ◽  
Vol 53 (38) ◽  
pp. 10237-10241 ◽  
Author(s):  
Ulrike Reinhardt ◽  
Jonathan Lotze ◽  
Sarah Zernia ◽  
Karin Mörl ◽  
Annette G. Beck-Sickinger ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Chemical Method ◽  
Acyl Transfer ◽  
Live Cells

Site-Selective Labeling of a Lysine Residue in Human Serum Albumin

2014 ◽  
Vol 126 (44) ◽  
pp. 11977-11980 ◽  
Author(s):  
Shigehiro Asano ◽  
James T. Patterson ◽  
Thomas Gaj ◽  
Carlos F. Barbas
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Lysine Residue ◽  
Selective Labeling ◽  
Site Selective

scholarly journals Sensitive and High Resolution Localization and Tracking of Membrane Proteins in Live Cells with BRET

Traffic ◽  
2012 ◽  
Vol 13 (11) ◽  
pp. 1450-1456 ◽  
Author(s):  
Tien-Hung Lan ◽  
Qiuju Liu ◽  
Chunman Li ◽  
Guangyu Wu ◽  
Nevin A. Lambert
Keyword(s):  
High Resolution ◽  
Membrane Proteins ◽  
Live Cells ◽  
Localization And Tracking
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