Faculty of 1000 evaluation for MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules.

SummaryA prospective, open-label multicenter investigation has been conducted to compare pharmacokinetic parameters of recombinant DNA-derived FVIII (rFVIII) and plasma-derived FVIII concentrate (pdFVIII) and to assess safety and efficacy of long-term home-treat- ment with rFVIII for subjects with hemophilia A. Following comparative in vivo pharmacokinetic studies, 69 patients with severe (n = 67) or moderate (n = 2) hemophilia A commenced a program of home treatment using rFVIII exclusively for prophylaxis and treatment of all bleeding episodes for a period of 1.0 to 5.7 years (median 3.7 years). The mean in vivo half-lives of rFVIII and pdFVIII were both 14.7 h. In vivo incremental recoveries at baseline were 2.40%/IU/kg and 2.47%/IU/kg, respectively (p = 0.59). The response to home treatment with rFVIII was categorized as good or excellent in 3,195 (91.2%) of 3,481 evaluated bleeding episodes. Thirteen patients received rFVIII for prophylaxis for twenty-four surgical procedures. In all cases, hemostasis was excellent. Adverse reactions were observed in only 13 of 13,591 (0.096%) infusions of rFVIII; none was serious. No patient developed an inhibitor to r FVIII.

Download Full-text

GENETIC HETEROZYGOSITY IN UNREPLICATED BACTERIOPHAGE Λ RECOMBINANTS

Genetics ◽

10.1093/genetics/81.1.33 ◽

1975 ◽

Vol 81 (1) ◽

pp. 33-50

Author(s):

Raymond L White ◽

Maurice S Fox

Keyword(s):

Dna Synthesis ◽

Base Pair ◽

Recombinant Dna ◽

Dna Molecules ◽

Bacteriophage Λ ◽

Negative Interference ◽

Genetic Interval ◽

Parental Material ◽

Genetic Heterozygosity ◽

Genetic Contributions

ABSTRACT Bacteriophage crosses using density-labeled parents have been carried out under conditions restricting DNA synthesis. The parental material and genetic contributions to progeny manifesting recombination within a genetic interval sufficiently short to exhibit high negative interference have been examined. The unreplicated products of recombination isolated as phage particles appear to contain long continuous heteroduplex regions which are heterozygous for the closely linked markers. Recombination between closely linked markers seems to be the consequence of the removal of base-pair mismatches that are present within the heteroduplex regions. This localized reduction of heterozygosity within the heteroduplex regions that join the parental components of recombinant DNA molecules can account for high negative interference.

Download Full-text

isoTarget: A Genetic Method for Analyzing the Functional Diversity of Splicing Isoforms In Vivo

Cell Reports ◽

10.1016/j.celrep.2020.108361 ◽

2020 ◽

Vol 33 (6) ◽

pp. 108361

Author(s):

Hao Liu ◽

Sarah Pizzano ◽

Ruonan Li ◽

Wenquan Zhao ◽

Macy W. Veling ◽

...

Keyword(s):

Functional Diversity ◽

Genetic Method ◽

Splicing Isoforms

Download Full-text

5200509 Human somatomedin carrier protein subunits and process for producing them; recombinant DNA molecules, hosts, processes and human somatomedin carrier protein-like polypeptides

Biotechnology Advances ◽

10.1016/0734-9750(94)90411-1 ◽

1994 ◽

Vol 12 (1) ◽

pp. 129

Keyword(s):

Recombinant Dna ◽

Carrier Protein ◽

Dna Molecules ◽

Protein Subunits

Download Full-text

Influenza virus hemagglutinin-specific cytotoxic T cell response induced by polypeptide produced in Escherichia coli.

Journal of Experimental Medicine ◽

10.1084/jem.162.2.663 ◽

1985 ◽

Vol 162 (2) ◽

pp. 663-674 ◽

Cited By ~ 29

Author(s):

A Yamada ◽

M R Ziese ◽

J F Young ◽

Y K Yamada ◽

F A Ennis

Keyword(s):

Influenza Virus ◽

Recombinant Dna ◽

Cytotoxic T Lymphocyte ◽

Nonstructural Protein ◽

T Cell Response ◽

Cytotoxic T Cell ◽

Protein Immunization ◽

Recombinant Dna Techniques

We have tested the abilities of various polypeptides of A/PR/8/34 (H1N1) virus, constructed by recombinant DNA techniques, to induce influenza virus-specific secondary cytotoxic T lymphocyte (CTL) responses. A hybrid protein (c13 protein), consisting of the first 81 amino acids of viral nonstructural protein (NS1) and the HA2 subunit of viral hemagglutinin (HA), induced H-2-restricted, influenza virus subtype-specific secondary CTL in vitro, although other peptides did not. Using a recombinant virus, the viral determinant responsible for recognition was mapped to the HA2 portion of c13 protein. Immunization of mice with c13 protein induced the generation of memory CTL in vivo. The CTL precursor frequencies of A/PR/8/34 virus- and c13 protein-immune mice were estimated as one in 8,047 and 50,312, respectively. These results indicate that c13 protein primed recipient mice, even though the level of precursor frequency was below that observed in virus-immune mice.

Download Full-text