Faculty Opinions recommendation of Probing long-distance regulatory interactions in the Drosophila melanogaster bithorax complex using Dam identification.

Abstract In the studies reported here, we have examined the properties of the Mcp element from the Drosophila melanogaster bithorax complex (BX-C). We have found that sequences from the Mcp region of BX-C have properties characteristic of Polycomb response elements (PREs), and that they silence adjacent reporters by a mechanism that requires trans-interactions between two copies of the transgene. However, Mcp trans-regulatory interactions have several novel features. In contrast to classical transvection, homolog pairing does not seem to be required. Thus, trans-regulatory interactions can be observed not only between Mcp transgenes inserted at the same site, but also between Mcp transgenes inserted at distant sites on the same chromosomal arm, or even on different arms. Trans-regulation can even be observed between transgenes inserted on different chromosomes. A small 800-bp Mcp sequence is sufficient to mediate these long-distance trans-regulatory interactions. This small fragment has little silencing activity on its own and must be combined with other Polycomb-Group-responsive elements to function as a “pairing-sensitive” silencer. Finally, this pairing element can also mediate long-distance interactions between enhancers and promoters, activating mini-white expression.

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Faculty Opinions recommendation of Probing long-distance regulatory interactions in the Drosophila melanogaster bithorax complex using Dam identification.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1033584.388829 ◽

2006 ◽

Author(s):

Bruce Morgan

Keyword(s):

Drosophila Melanogaster ◽

Bithorax Complex ◽

Long Distance ◽

Regulatory Interactions

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Probing long-distance regulatory interactions in the Drosophila melanogaster bithorax complex using Dam identification

Nature Genetics ◽

10.1038/ng1833 ◽

2006 ◽

Vol 38 (8) ◽

pp. 931-935 ◽

Cited By ~ 86

Author(s):

Fabienne Cléard ◽

Yuri Moshkin ◽

François Karch ◽

Robert K Maeda

Keyword(s):

Drosophila Melanogaster ◽

Bithorax Complex ◽

Long Distance ◽

Regulatory Interactions

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The bithorax complex of Drosophila melanogaster as a model for studying specific long-distance interactions between enhancers and promoters

Russian Journal of Genetics ◽

10.1134/s1022795415050038 ◽

2015 ◽

Vol 51 (5) ◽

pp. 440-448 ◽

Cited By ~ 1

Author(s):

O. V. Kyrchanova ◽

P. G. Georgiev

Keyword(s):

Drosophila Melanogaster ◽

Bithorax Complex ◽

Long Distance

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Replacement of Fab-7 by the gypsy or scs Insulator Disrupts Long-Distance Regulatory Interactions in the Abd-B Gene of the Bithorax Complex

Molecular Cell ◽

10.1016/s1097-2765(01)00377-x ◽

2001 ◽

Vol 8 (5) ◽

pp. 1145-1151 ◽

Cited By ~ 34

Author(s):

Ilham Hogga ◽

Jozsef Mihaly ◽

Stéphane Barges ◽

François Karch

Keyword(s):

Bithorax Complex ◽

Long Distance ◽

Regulatory Interactions

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Perturbation of chromatin architecture on ecdysterone induction of Drosophila melanogaster small heat shock protein genes.

Molecular and Cellular Biology ◽

10.1128/mcb.9.1.332 ◽

1989 ◽

Vol 9 (1) ◽

pp. 332-335 ◽

Cited By ~ 7

Author(s):

S E Kelly ◽

I L Cartwright

Keyword(s):

Drosophila Melanogaster ◽

Heat Shock ◽

Heat Shock Protein ◽

Intergenic Region ◽

Small Heat Shock Protein ◽

Dnase I ◽

Developmentally Regulated ◽

Regulatory Interactions ◽

Chromatin Architecture ◽

Heat Shock Protein Genes

Alterations in the pattern of DNase I hypersensitivity were observed on ecdysterone-stimulated transcription of Drosophila melanogaster small heat shock protein genes. Perturbations were induced near hsp27 and hsp22, coupled with an extensive domain of chromatin unfolding in the intergenic region between hsp23 and the developmentally regulated gene 1. These regions represent candidates for ecdysterone regulatory interactions.

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The origin of pattern duplications in segment polarity mutants of Drosophila melanogaster

Development ◽

10.1242/dev.87.1.129 ◽

1985 ◽

Vol 87 (1) ◽

pp. 129-135

Author(s):

Alfonso Martinez-Arias ◽

Philip W. Ingham

Keyword(s):

Drosophila Melanogaster ◽

Bithorax Complex ◽

Anterior Compartment ◽

Larval Cuticle ◽

Segment Polarity ◽

Reversed Polarity ◽

Segmental Identity

Mutations of the segment polarity group in Drosophila melanogaster produce additional denticles with reversed polarity in every segment of the larval cuticle. We have investigated the effect of mutations in different elements of the bithorax complex on the segmental identity of these additional pattern elements. Our results suggest that they are derived, primarily, from the anterior compartment of each segment.

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The enhancer-blocking suppressor of Hairy-wing zinc finger protein of Drosophila melanogaster alters DNA structure

Molecular and Cellular Biology ◽

10.1128/mcb.14.9.5645-5652.1994 ◽

1994 ◽

Vol 14 (9) ◽

pp. 5645-5652

Author(s):

B Shen ◽

J Kim ◽

D Dorsett

Keyword(s):

Drosophila Melanogaster ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Dna Structure ◽

Protein Domain ◽

Transcription Activator ◽

Long Distance ◽

Dna Flexibility ◽

Finger Protein ◽

Enhancer Blocking

Insertion of the gypsy retrotransposon of Drosophila melanogaster into a gene control region can repress gene expression. The zinc finger protein (SUHW) encoded by the suppressor of Hairy-wing [su(Hw)] gene binds to gypsy and prevents gene enhancers from activating transcription. SUHW blocks an enhancer only when positioned between the enhancer and promoter. Although position dependent, SUHW enhancer blocking is distance independent. These properties indicate that SUHW does not interact with the transcription activator proteins that bind to enhancers. To explore if DNA distortions are involved in enhancer blocking, the ability of SUHW to alter DNA structure was examined in gel mobility assays. Indeed, SUHW induces an unusual change in the structure of the binding-site DNA. The change is not a directed DNA bend but correlates with loss of sequence-directed bends in the unbound DNA. The DNA distortion requires a SUHW protein domain not required for DNA binding, and mutant proteins that fail to alter DNA structure also fail to eliminate the sequence-directed bends. These results suggest that SUHW increases DNA flexibility. The DNA distortion is not sufficient to block enhancers, and therefore it is suggested that increased DNA flexibility may help SUHW interact and interfere with proteins that support long-distance enhancer-promoter interactions.

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The pattern of campaniform sensilla on the wing and haltere of Drosophila melanogaster and several of its homeotic mutants

Development ◽

10.1242/dev.71.1.41 ◽

1982 ◽

Vol 71 (1) ◽

pp. 41-61

Author(s):

Eric S. Cole ◽

John Palka

Keyword(s):

Drosophila Melanogaster ◽

Campaniform Sensilla ◽

Bithorax Complex ◽

Homeotic Transformation ◽

Anterior Compartment

A detailed mapping and description of campaniform sensilla on the wing and haltere of Drosophila melanogaster is provided. Six types of sensilla are distinguished. Similarities in the pattern of their distribution on the dorsal and ventral surfaces of each appendage, as well as between the wing and haltere, are apparent. These data are used to assess the quality of homeotic transformation in several mutants of the bithorax complex in which the halteres are transformed into wings. Flies homozygous for abxbx3pbx produce a complete inventory of wing sensilla on the homeotic appendage. In abx, bx3 and bx3pbx homozygotes the transformation of haltere into wing is incomplete, and each mutant shows characteristic fields of haltere and wing sensilla. It appears that specific regions of the anterior haltere compartment require different combinations of mutant alleles to produce a distinct homeotic transformation. Furthermore, the pbx mutation appears to influence expression of the bx3 mutation within the anterior compartment.

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