scholarly journals Replacement of Fab-7 by the gypsy or scs Insulator Disrupts Long-Distance Regulatory Interactions in the Abd-B Gene of the Bithorax Complex

2001 ◽  
Vol 8 (5) ◽  
pp. 1145-1151 ◽  
Author(s):  
Ilham Hogga ◽  
Jozsef Mihaly ◽  
Stéphane Barges ◽  
François Karch
Keyword(s):  
Bithorax Complex ◽  
Long Distance ◽  
Regulatory Interactions

scholarly journals The Mcp Element From the Drosophila melanogaster Bithorax Complex Mediates Long-Distance Regulatory Interactions

Genetics ◽  
1999 ◽  
Vol 153 (3) ◽  
pp. 1333-1356 ◽  
Author(s):  
Martin Muller ◽  
Kirsten Hagstrom ◽  
Henrik Gyurkovics ◽  
Vincenzo Pirrotta ◽  
Paul Schedl
Keyword(s):  
Drosophila Melanogaster ◽  
Polycomb Group ◽  
Small Fragment ◽  
Bithorax Complex ◽  
Long Distance ◽  
Response Elements ◽  
Regulatory Interactions ◽  
Homolog Pairing ◽  
Polycomb Response Elements ◽  
Trans Regulation

Abstract In the studies reported here, we have examined the properties of the Mcp element from the Drosophila melanogaster bithorax complex (BX-C). We have found that sequences from the Mcp region of BX-C have properties characteristic of Polycomb response elements (PREs), and that they silence adjacent reporters by a mechanism that requires trans-interactions between two copies of the transgene. However, Mcp trans-regulatory interactions have several novel features. In contrast to classical transvection, homolog pairing does not seem to be required. Thus, trans-regulatory interactions can be observed not only between Mcp transgenes inserted at the same site, but also between Mcp transgenes inserted at distant sites on the same chromosomal arm, or even on different arms. Trans-regulation can even be observed between transgenes inserted on different chromosomes. A small 800-bp Mcp sequence is sufficient to mediate these long-distance trans-regulatory interactions. This small fragment has little silencing activity on its own and must be combined with other Polycomb-Group-responsive elements to function as a “pairing-sensitive” silencer. Finally, this pairing element can also mediate long-distance interactions between enhancers and promoters, activating mini-white expression.

Probing long-distance regulatory interactions in the Drosophila melanogaster bithorax complex using Dam identification

2006 ◽  
Vol 38 (8) ◽  
pp. 931-935 ◽  
Author(s):  
Fabienne Cléard ◽  
Yuri Moshkin ◽  
François Karch ◽  
Robert K Maeda
Keyword(s):  
Drosophila Melanogaster ◽  
Bithorax Complex ◽  
Long Distance ◽  
Regulatory Interactions

scholarly journals Promoter–enhancer tethering is critical for long-range regulatory interactions in the bithorax complex of Drosophila

2009 ◽  
Vol 331 (2) ◽  
pp. 435-436
Author(s):  
Margaret C. Ho ◽  
Benjamin J. Schiller ◽  
Omar S. Akbari ◽  
Esther Bae ◽  
Robert A. Drewell
Keyword(s):  
Long Range ◽  
Bithorax Complex ◽  
Regulatory Interactions

scholarly journals Boundaries support specific long-distance interactions between enhancers and promoters in Drosophila Bithorax complex

2018 ◽  
Author(s):  
Nikolay Postika ◽  
Mario Metzler ◽  
Markus Affolter ◽  
Martin Müller ◽  
Paul Schedl ◽  
...  
Keyword(s):  
Target Genes ◽  
Model Systems ◽  
Homeotic Genes ◽  
Bithorax Complex ◽  
Long Distance ◽  
Gene Target ◽  
Regulatory Domains ◽  
Insulator Elements

AbstractDrosophila bithorax complex (BX-C) is one of the best model systems for studying the role of boundaries (insulators) in gene regulation. Expression of three homeotic genes, Ubx, abd-A, and Abd-B, is orchestrated by nine parasegment-specific regulatory domains. These domains are flanked by boundary elements, which function to block crosstalk between adjacent domains, ensuring that they can act autonomously. Paradoxically, seven of the BX-C regulatory domains are separated from their gene target by at least one boundary, and must “jump over” the intervening boundaries. To understand the jumping mechanism, the Mcp boundary was replaced with Fab-7 and Fab-8. Mcp is located between the iab-4 and iab-5 domains, and defines the border between the set of regulatory domains controlling abd-A and Abd-B. When Mcp is replaced by Fab-7 or Fab-8, they direct the iab-4 domain (which regulates abd-A) to inappropriately activate Abd-B in abdominal segment A4. For the Fab-8 replacement, ectopic induction was only observed when it was inserted in the same orientation as the endogenous Fab-8 boundary. A similar orientation dependence for bypass activity was observed when Fab-7 was replaced by Fab-8. Thus, boundaries perform two opposite functions in the context of BX-C – they block crosstalk between neighboring regulatory domains, but at the same time actively facilitate long distance communication between the regulatory domains and their respective target genes.Author SummaryDrosophila bithorax complex (BX-C) is one of a few examples demonstrating in vivo role of boundary/insulator elements in organization of independent chromatin domains. BX-C contains three HOX genes, whose parasegment-specific pattern is controlled by cis-regulatory domains flanked by boundary/insulator elements. Since the boundaries ensure autonomy of adjacent domains, the presence of these elements poses a paradox: how do the domains bypass the intervening boundaries and contact their proper regulatory targets? According to the textbook model, BX-C regulatory domains are able to bypass boundaries because they harbor special promoter targeting sequences. However, contrary to this model, we show here that the boundaries themselves play an active role in directing regulatory domains to their appropriate HOX gene promoter.

scholarly journals Complete reconstitution of bypass and blocking functions in a minimal artificial Fab-7 insulator from Drosophila bithorax complex

2019 ◽  
Vol 116 (27) ◽  
pp. 13462-13467 ◽  
Author(s):  
Olga Kyrchanova ◽  
Marat Sabirov ◽  
Vladic Mogila ◽  
Amina Kurbidaeva ◽  
Nikolay Postika ◽  
...  
Keyword(s):  
Cross Talk ◽  
Binding Sites ◽  
Multiprotein Complex ◽  
Bithorax Complex ◽  
Artificial Boundary ◽  
Long Distance ◽  
Specific Expression ◽  
Regulatory Domains ◽  
Architectural Proteins ◽  
Distance Communication

Boundaries in the bithorax complex (BX-C) delimit autonomous regulatory domains that drive parasegment-specific expression of the Hox genes Ubx, abd-A, and Abd-B. The Fab-7 boundary is located between the iab-6 and iab-7 domains and has two key functions: blocking cross-talk between these domains and at the same time promoting communication (boundary bypass) between iab-6 and the Abd-B promoter. Using a replacement strategy, we found that multimerized binding sites for the architectural proteins Pita, Su(Hw), and dCTCF function as conventional insulators and block cross-talk between the iab-6 and iab-7 domains; however, they lack bypass activity, and iab-6 is unable to regulate Abd-B. Here we show that an ∼200-bp sequence of dHS1 from the Fab-7 boundary rescues the bypass defects of these multimerized binding sites. The dHS1 sequence is bound in embryos by a large multiprotein complex, Late Boundary Complex (LBC), that contains the zinc finger proteins CLAMP and GAF. Using deletions and mutations in critical GAGAG motifs, we show that bypass activity correlates with the efficiency of recruitment of LBC components CLAMP and GAF to the artificial boundary. These results indicate that LBC orchestrates long-distance communication between the iab-6 regulatory domain and the Abd-B gene, while the Pita, Su(Hw), and dCTCF proteins function to block local cross-talk between the neighboring regulatory domains iab-6 and iab-7.

scholarly journals Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex

PLoS Genetics ◽  
2018 ◽  
Vol 14 (12) ◽  
pp. e1007702 ◽  
Author(s):  
Nikolay Postika ◽  
Mario Metzler ◽  
Markus Affolter ◽  
Martin Müller ◽  
Paul Schedl ◽  
...  
Keyword(s):  
Bithorax Complex ◽  
Long Distance
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