Faculty Opinions recommendation of Notch activation enhances IFNgamma secretion by human peripheral blood and decidual NK cells.

2010 ◽  
Author(s):  
Thaddeus Golos ◽  
Svetlana Dambaeva
Keyword(s):  
Nk Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Notch Activation

Notch activation enhances IFNγ secretion by human peripheral blood and decidual NK cells

2010 ◽  
Vol 84 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Irit Manaster ◽  
Roi Gazit ◽  
Debra Goldman-Wohl ◽  
Noam Stern-Ginossar ◽  
Saar Mizrahi ◽  
...  
Keyword(s):  
Nk Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Notch Activation

Efficient and Reproducible Retroviral Infection of Primary Human Natural Killer Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1348-1348
Author(s):  
Brian Becknell ◽  
Rossana Trotta ◽  
Jianhua Yu ◽  
Wei Ding ◽  
Hsiaoyin C. Mao ◽  
...  
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Peripheral Blood ◽  
Targeted Delivery ◽  
Magnetic Beads ◽  
Nk Cell ◽  
Human Peripheral Blood ◽  
Cell Physiology ◽  
Gene Products ◽  
Successful Infection

Abstract Molecular characterization of human natural killer (NK) cells will require targeted gene delivery to inhibit and activate specific signaling pathways, yet to our knowledge, an effective means to deliver such products for long-term gene expression without disrupting normal cellular processes has not been described. In this study we have developed a retroviral strategy to effectively express gene products in the NK cell, whereby its effector functions of cytotoxicity and cytokine production remain intact. Using an EBV/retroviral hybrid vector PINCO, we demonstrate infection of human peripheral blood NK cells with simultaneous expression of a marker for infection - the enhanced green fluorescent protein (EGFP) - along with various genes of interest. This technique results in successful infection of the CD56dim NK population that predominates among human peripheral blood NK and is the effector of antibody-dependent cellular cytotoxicity (ADCC) and natural killing. In addition, we demonstrate infection of the CD56bright NK subset as well as the NK-92 and NK-L cell lines. Finally, we modify PINCO to express a cytoplasmically truncated murine CD8 molecule in place of GFP. The resulting vector enables us to transduce NK cells with multiple genes of interest simultaneously and provides an alternative purification method to FACS by using magnetic beads. In summary, we have devised an efficient and highly reproducible methodology for the targeted delivery of gene products to human NK cells that should now provide opportunities to dissect the molecular processes critical to normal NK cell physiology and to genetically manipulate NK cell populations prior to their administration in cancer therapy.

scholarly journals Response of resting human peripheral blood natural killer cells to interleukin 2.

1984 ◽  
Vol 160 (4) ◽  
pp. 1147-1169 ◽  
Author(s):  
G Trinchieri ◽  
M Matsumoto-Kobayashi ◽  
S C Clark ◽  
J Seehra ◽  
L London ◽  
...  
Keyword(s):  
T Cells ◽  
Nk Cells ◽  
Natural Killer ◽  
Peripheral Blood ◽  
Nk Cell ◽  
Human Peripheral Blood ◽  
Interleukin 2 ◽  
Cell Activity ◽  
Experimental Conditions ◽  
Ifn Gamma

The present study shows that recombinant interleukin 2 (IL-2) purified to homogeneity induces a rapid and potent enhancement of spontaneous cytotoxicity of human peripheral blood lymphocytes. The cells mediating cytotoxicity after 18-h treatment with IL-2 have surface markers of natural killer (NK) cells and are generated from the peripheral blood subset containing spontaneous cytotoxic cells. A parallel production of gamma interferon (IFN-gamma) is induced by recombinant IL-2 (rIL-2), and NK cells appear to be the major producer cells, whereas T cells are unable to produce IFN-gamma under these experimental conditions. However, the kinetics of the enhancement of cytotoxicity are faster than those of IFN-gamma production, and monoclonal anti-IFN-gamma antibodies do not suppress this effect, making it unlikely that the IFN-gamma produced is responsible for the enhancement. The enhancement of NK cell activity induced by rIL-2 precedes any proliferative response of the lymphocytes, which is instead observed in longer-term cultures of both NK and T cells.

scholarly journals Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

10.3791/2540 ◽  
2011 ◽  
Author(s):  
Srinivas S. Somanchi ◽  
Vladimir V. Senyukov ◽  
Cecele J. Denman ◽  
Dean A. Lee
Keyword(s):  
Nk Cells ◽  
Functional Assessment ◽  
Peripheral Blood ◽  
Human Peripheral Blood

HUMAN PERIPHERAL BLOOD DNAM-1NEG NK CELLS ARE A TERMINALLY DIFFERENTIATED SUBSET WITH IMMUNOREGULATORY PROPERTIES

2019 ◽  
Vol 76 ◽  
pp. S68
Author(s):  
Camille Guillerey ◽  
Kimberley Stannard ◽  
Sebastien Lemoine ◽  
Nigel Waterhouse ◽  
Frank Vari ◽  
...  
Keyword(s):  
Nk Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood

scholarly journals Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells

2002 ◽  
Vol 195 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Franca Gerosa ◽  
Barbara Baldani-Guerra ◽  
Carla Nisii ◽  
Viviana Marchesini ◽  
Giuseppe Carra ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Nk Cells ◽  
Natural Killer ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Cytolytic Activity ◽  
Cell Contact ◽  
Immature Dendritic Cells ◽  
Dc Maturation

We analyzed the interaction between human peripheral blood natural killer (NK) cells and monocyte-derived immature dendritic cells (DC). Fresh NK cells were activated, as indicated by the induced expression of the CD69 antigen, and their cytolytic activity was strongly augmented by contact with lipopolysaccharide (LPS)-treated mature DC, or with immature DC in the presence of the maturation stimuli LPS, Mycobacterium tuberculosis or interferon (IFN)-α. Reciprocally, fresh NK cells cultured with immature DC in the presence of the maturation stimuli strongly enhanced DC maturation and interleukin (IL)-12 production. IL-2–activated NK cells directly induced maturation of DC and enhanced their ability to stimulate allogeneic naive CD4+ T cells. The effects of NK cells were cell contact dependent, although the secretion of IFN-γ and TNF also contributed to DC maturation. Within peripheral blood lymphocytes the reciprocal activating interaction with DC was restricted to NK cells, because the other lymphocyte subsets were neither induced to express CD69, nor induced to mature in contact with DC. These data demonstrated for the first time a bidirectional cross talk between NK cells and DC, in which NK cells activated by IL-2 or by mature DC induce DC maturation.

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