Faculty Opinions recommendation of Genome-wide analysis of estrogen receptor alpha DNA binding and tethering mechanisms identifies Runx1 as a novel tethering factor in receptor-mediated transcriptional activation.

2010 ◽  
Author(s):  
Mark Hahn
Keyword(s):  
Estrogen Receptor ◽  
Dna Binding ◽  
Transcriptional Activation ◽  
Estrogen Receptor Alpha ◽  
Genome Wide Analysis ◽  
Receptor Alpha ◽  
Genome Wide

scholarly journals Genome-Wide Analysis of Estrogen Receptor α DNA Binding and Tethering Mechanisms Identifies Runx1 as a Novel Tethering Factor in Receptor-Mediated Transcriptional Activation

2010 ◽  
Vol 30 (16) ◽  
pp. 3943-3955 ◽  
Author(s):  
Joshua D. Stender ◽  
Kyuri Kim ◽  
Tze Howe Charn ◽  
Barry Komm ◽  
Ken C. N. Chang ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Estrogen Receptor ◽  
Dna Binding ◽  
Transcriptional Activation ◽  
Estrogen Receptor Alpha ◽  
High Throughput Sequencing ◽  
De Novo ◽  
Motif Analysis ◽  
Phenotypic Properties ◽  
Genome Wide

ABSTRACT Nuclear receptor estrogen receptor alpha (ERα) controls the expression of hundreds of genes responsible for target cell phenotypic properties, but the relative importance of direct versus tethering mechanisms of DNA binding has not been established. In this first report, we examine the genome-wide chromatin localization of an altered-specificity mutant ER with a DNA binding domain deficient in binding to estrogen response element (ERE)-containing DNA (DBDmut ER) versus wild-type ERα. Using high-throughput sequencing of ER chromatin immunoprecipitations (ChIP-Seq) and mRNA transcriptional profiling, we show that direct ERE binding is required for most of (75%) estrogen-dependent gene regulation and 90% of hormone-dependent recruitment of ER to genomic binding sites. De novo motif analysis of the chromatin binding regions in MDA-MB-231 human breast cancer cells defined unique transcription factor profiles responsible for genes regulated through tethering versus direct ERE binding, with Runx motifs enriched in ER-tethered sites. We confirmed a role for Runx1 in mediating ERα genomic recruitment and regulation of tethering genes. Our findings delineate the contributions of direct receptor ERE binding versus binding through response elements for other transcription factors in chromatin localization and ER-dependent gene regulation, paradigms likely to underlie the gene regulatory actions of other nuclear receptors as well.

scholarly journals Crosstalk between Hormone and DNA Binding Domains in Estrogen Receptor Alpha: An Energy Landscape Approach

2013 ◽  
Vol 104 (2) ◽  
pp. 70a
Author(s):  
Wei Huang ◽  
Krishnakumar M. Ravikumar ◽  
Sichun Yang
Keyword(s):  
Estrogen Receptor ◽  
Dna Binding ◽  
Estrogen Receptor Alpha ◽  
Energy Landscape ◽  
Landscape Approach ◽  
Receptor Alpha ◽  
Dna Binding Domains ◽  
Binding Domains

Requirement of protein l-isoaspartyl O-methyltransferase for transcriptional activation of trefoil factor 1 (TFF1) gene by estrogen receptor alpha

2012 ◽  
Vol 420 (2) ◽  
pp. 223-229 ◽  
Author(s):  
Jong Woo Park ◽  
Jae Cheol Lee ◽  
Shin Won Ha ◽  
So Young Bang ◽  
Eun Kyung Park ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Transcriptional Activation ◽  
Estrogen Receptor Alpha ◽  
Trefoil Factor ◽  
Protein L ◽  
Receptor Alpha ◽  
Trefoil Factor 1

scholarly journals Requirement of estrogen receptor alpha DNA-binding domain for HPV oncogene-induced cervical carcinogenesis in mice

2013 ◽  
Vol 35 (2) ◽  
pp. 489-496 ◽  
Author(s):  
J. Son ◽  
J. W. Park ◽  
P. F. Lambert ◽  
S.-H. Chung
Keyword(s):  
Estrogen Receptor ◽  
Dna Binding ◽  
Estrogen Receptor Alpha ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Cervical Carcinogenesis ◽  
Receptor Alpha

scholarly journals Age-specific changes in genome-wide methylation enrich for Foxa2 and estrogen receptor alpha binding sites

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0203147 ◽  
Author(s):  
Nishanth Uli ◽  
Eduardo Michelen-Gomez ◽  
Enrique I. Ramos ◽  
Todd E. Druley
Keyword(s):  
Estrogen Receptor ◽  
Binding Sites ◽  
Estrogen Receptor Alpha ◽  
Receptor Alpha ◽  
Genome Wide

scholarly journals Genome-wide mapping of FOXM1 binding reveals co-binding with estrogen receptor alpha in breast cancer cells

2013 ◽  
Vol 14 (1) ◽  
pp. R6 ◽  
Author(s):  
Deborah A Sanders ◽  
Caryn S Ross-Innes ◽  
Dario Beraldi ◽  
Jason S Carroll ◽  
Shankar Balasubramanian
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Estrogen Receptor Alpha ◽  
Receptor Alpha ◽  
Genome Wide

scholarly journals Mechanisms of transcriptional activation of the mouse claudin-5 promoter by estrogen receptor alpha and beta

2014 ◽  
Vol 392 (1-2) ◽  
pp. 144-151 ◽  
Author(s):  
Malgorzata Burek ◽  
Katrin Steinberg ◽  
Carola Y. Förster
Keyword(s):  
Estrogen Receptor ◽  
Transcriptional Activation ◽  
Estrogen Receptor Alpha ◽  
Receptor Alpha
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