Faculty Opinions recommendation of Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography.

2015 ◽  
Author(s):  
Satyajit Mayor
Keyword(s):  
Plasma Membrane ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Membrane Skeleton ◽  
Three Dimensional Reconstruction ◽  
Membrane Interface ◽  
Dimensional Reconstruction

Three-dimensional reconstruction of axonemal outer dynein arms in situ by electron tomography

2005 ◽  
Vol 62 (2) ◽  
pp. 69-83 ◽  
Author(s):  
Pietro Lupetti ◽  
Salvatore Lanzavecchia ◽  
David Mercati ◽  
Francesca Cantele ◽  
Romano Dallai ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
Dynein Arms

Three-dimensional reconstruction of collagen–proteoglycan interactions in the mouse corneal stroma by electron tomography

2010 ◽  
Vol 170 (2) ◽  
pp. 392-397 ◽  
Author(s):  
Geraint J. Parfitt ◽  
Christian Pinali ◽  
Robert D. Young ◽  
Andrew J. Quantock ◽  
Carlo Knupp
Keyword(s):  
Electron Tomography ◽  
Three Dimensional ◽  
Corneal Stroma ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction

scholarly journals Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography

2006 ◽  
Vol 174 (6) ◽  
pp. 851-862 ◽  
Author(s):  
Nobuhiro Morone ◽  
Takahiro Fujiwara ◽  
Kotono Murase ◽  
Rinshi S. Kasai ◽  
Hiroshi Ike ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Actin Filaments ◽  
High Speed ◽  
Plasma Membranes ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Membrane Skeleton ◽  
Single Particle Tracking ◽  
Coated Pits ◽  
Cytoplasmic Surface

Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal rat skin keratinocytes were reconstructed by electron tomography, with 0.85-nm–thick consecutive sections made ∼100 nm from the cytoplasmic surface using rapidly frozen, deeply etched, platinum-replicated plasma membranes. The membrane skeleton (MSK) primarily consists of actin filaments and associated proteins. The MSK covers the entire cytoplasmic surface and is closely linked to clathrin-coated pits and caveolae. The actin filaments that are closely apposed to the cytoplasmic surface of the plasma membrane (within 10.2 nm) are likely to form the boundaries of the membrane compartments responsible for the temporary confinement of membrane molecules, thus partitioning the plasma membrane with regard to their lateral diffusion. The distribution of the MSK mesh size as determined by electron tomography and that of the compartment size as determined from high speed single-particle tracking of phospholipid diffusion agree well in both cell types, supporting the MSK fence and MSK-anchored protein picket models.

Serial Section Electron Tomography: A Method for Three-Dimensional Reconstruction of Large Structures

NeuroImage ◽  
1994 ◽  
Vol 1 (3) ◽  
pp. 230-243 ◽  
Author(s):  
Gabriel E. Soto ◽  
Stephen J. Young ◽  
Maryann E. Martone ◽  
Thomas J. Deerinck ◽  
Stephan Lamont ◽  
...  
Keyword(s):  
Serial Section ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Large Structures ◽  
Dimensional Reconstruction ◽  
Section Electron
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