scholarly journals 2S2-2 Three dimensional interplay of the membrane skeleton with the plasma membrane as visualized by freeze-etch electron tomography(2S2 Interactions between the cell membrane and the actin cytoskeleton: Biophysical approaches,The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S8
Author(s):  
Nobuhiro Morone ◽  
Takahiro K. Fujiwara ◽  
Rinshi S. Kasai ◽  
Shigeki Yuasa ◽  
Jiro Usukura ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cell Membrane ◽  
Actin Cytoskeleton ◽  
Annual Meeting ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Membrane Skeleton ◽  
Biophysical Society

scholarly journals Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography

2006 ◽  
Vol 174 (6) ◽  
pp. 851-862 ◽  
Author(s):  
Nobuhiro Morone ◽  
Takahiro Fujiwara ◽  
Kotono Murase ◽  
Rinshi S. Kasai ◽  
Hiroshi Ike ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Actin Filaments ◽  
High Speed ◽  
Plasma Membranes ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Membrane Skeleton ◽  
Single Particle Tracking ◽  
Coated Pits ◽  
Cytoplasmic Surface

Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal rat skin keratinocytes were reconstructed by electron tomography, with 0.85-nm–thick consecutive sections made ∼100 nm from the cytoplasmic surface using rapidly frozen, deeply etched, platinum-replicated plasma membranes. The membrane skeleton (MSK) primarily consists of actin filaments and associated proteins. The MSK covers the entire cytoplasmic surface and is closely linked to clathrin-coated pits and caveolae. The actin filaments that are closely apposed to the cytoplasmic surface of the plasma membrane (within 10.2 nm) are likely to form the boundaries of the membrane compartments responsible for the temporary confinement of membrane molecules, thus partitioning the plasma membrane with regard to their lateral diffusion. The distribution of the MSK mesh size as determined by electron tomography and that of the compartment size as determined from high speed single-particle tracking of phospholipid diffusion agree well in both cell types, supporting the MSK fence and MSK-anchored protein picket models.

scholarly journals 2P-022 Investigating protein three-dimensional structures inside living cells by in-cell NMR spectroscopy(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S78
Author(s):  
Junpei Hamatsu ◽  
Daisuke Sakakibara ◽  
Atsuko Sasaki ◽  
Teppei Ikeya ◽  
Masaki Mishima ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Annual Meeting ◽  
Three Dimensional ◽  
Living Cells ◽  
Biophysical Society

scholarly journals 2K1524 Regulation mechanism for signal propagation along the plasma membrane : a single-molecule tracking study(Cell biology 2,The 48th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S93-S94
Author(s):  
Koichiro M. Hirosawa ◽  
Kenta J. Yoshida ◽  
Kenichi G. N. Suzuki ◽  
Takahiro K. Fujiwara ◽  
Ankita Chadda ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Annual Meeting ◽  
Single Molecule ◽  
Cell Biology ◽  
Signal Propagation ◽  
Regulation Mechanism ◽  
Single Molecule Tracking ◽  
Biophysical Society
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