Faculty Opinions recommendation of Dynamic mRNA Transport and Local Translation in Radial Glial Progenitors of the Developing Brain.

2017 ◽  
Author(s):  
Jacqueline Trotter
Keyword(s):  
Developing Brain ◽  
Local Translation ◽  
Mrna Transport ◽  
Glial Progenitors ◽  
Radial Glial

scholarly journals Dynamic mRNA Transport and Local Translation in Radial Glial Progenitors of the Developing Brain

2016 ◽  
Vol 26 (24) ◽  
pp. 3383-3392 ◽  
Author(s):  
Louis-Jan Pilaz ◽  
Ashley L. Lennox ◽  
Jeremy P. Rouanet ◽  
Debra L. Silver
Keyword(s):  
Developing Brain ◽  
Local Translation ◽  
Mrna Transport ◽  
Glial Progenitors ◽  
Radial Glial

scholarly journals Distinct progenitor behavior underlying neocortical gliogenesis related to tumorigenesis

2020 ◽  
Author(s):  
Zhongfu Shen ◽  
Yang Lin ◽  
Jiajun Yang ◽  
David J. Jörg ◽  
Yuwei Peng ◽  
...  
Keyword(s):  
Progenitor Cell ◽  
Neurofibromatosis Type ◽  
Primary Brain Tumor ◽  
Precursor Cells ◽  
Cell Behavior ◽  
Neocortical Neurons ◽  
Glial Progenitors ◽  
Radial Glial ◽  
Oligodendrocyte Precursor

SUMMARYRadial glial progenitors (RGPs) are responsible for producing the vast majority of neurons and glia in the neocortex. While RGP behavior and progressive generation of neocortical neurons have been delineated, the exact process of neocortical gliogenesis remains elusive. Here, we report the precise progenitor cell behavior and gliogenesis program at single-cell resolution in the mouse neocortex. RGPs transition from neurogenesis to gliogenesis progressively, producing astrocytes, oligodendrocytes, or both in well-defined propensities of 60%:15%:25%, respectively, via fate-restricted “intermediate” precursor cells. While the total number of precursor cells generated by individual RGPs appears stochastic, the output of individual precursor cells exhibit clear patterns in number and subtype, and form discrete local subclusters. Clonal loss of tumor suppressor Neurofibromatosis type 1 leads to excessive production of glia selectively, especially oligodendrocyte precursor cells. These results delineate the cellular program of neocortical gliogenesis quantitatively and suggest the cellular and lineage origin of primary brain tumor.

Radial ‘glial’ progenitors: neurogenesis and signaling

2005 ◽  
Vol 15 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Leah Ever ◽  
Nicholas Gaiano
Keyword(s):  
Glial Progenitors ◽  
Radial Glial

scholarly journals Subcellular mRNA localization and local translation of Arhgap11a in radial glial cells regulates cortical development

2020 ◽  
Author(s):  
Louis-Jan Pilaz ◽  
Kaumudi Joshi ◽  
Jing Liu ◽  
Yuji Tsunekawa ◽  
Fernando Alsina ◽  
...  
Keyword(s):  
Glial Cells ◽  
Mrna Localization ◽  
Local Translation ◽  
Control Of Gene Expression ◽  
Radial Glial Cells ◽  
Rhoa Activity ◽  
Cis Element ◽  
Control Brain ◽  
Radial Glial ◽  
Cellular Compartments

mRNA localization and local translation enable exquisite spatial and temporal control of gene expression, particularly in highly polarized and elongated cells. These features are especially prominent in radial glial cells (RGCs), which serve as neural and glial precursors of the developing cerebral cortex, and scaffolds for migrating neurons. Yet the mechanisms by which distinct sub-cellular compartments of RGCs accomplish their diverse functions are poorly understood. Here, we demonstrate that subcellular RNA localization and translation of the RhoGAP Arhgap11a controls RGC morphology and mediates cortical cytoarchitecture. Arhgap11a mRNA and protein exhibit conserved localization to RGC basal structures in mice and humans, conferred by a 5′UTR cis-element. Proper RGC morphology relies upon active Arhgap11a mRNA transport and localization to basal structures, where ARHGAP11A is locally synthesized. Thus, RhoA activity is spatially and acutely activated via local translation in RGCs to promote neuron positioning and cortical cytoarchitecture. Altogether, our study demonstrates that mRNA localization and local translation mediate compartmentalization of neural progenitor functions to control brain development.

Loss of PP2A Disrupts the Retention of Radial Glial Progenitors in the Telencephalic Niche to Impair the Generation for Late-Born Neurons During Cortical Development†

2020 ◽  
Vol 30 (7) ◽  
pp. 4183-4196
Author(s):  
Chaoli Huang ◽  
Tingting Liu ◽  
Qihui Wang ◽  
Weikang Hou ◽  
Cuihua Zhou ◽  
...  
Keyword(s):  
Neural Progenitor Cells ◽  
Ventricular Zone ◽  
Cortical Development ◽  
Underlying Mechanism ◽  
Conditional Knockout ◽  
Glial Progenitors ◽  
Radial Glial ◽  
Vitro Analysis ◽  
The Impact

Abstract Telencephalic radial glial progenitors (RGPs) are retained in the ventricular zone (VZ), the niche for neural stem cells during cortical development. However, the underlying mechanism is not well understood. To study whether protein phosphatase 2A (PP2A) may regulate the above process, we generate Ppp2cα conditional knockout (cKO) mice, in which PP2A catalytic subunit α (PP2Acα) is inactivated in neural progenitor cells in the dorsal telencephalon. We show that RGPs are ectopically distributed in cortical areas outside of the VZ in Ppp2cα cKO embryos. Whereas deletion of PP2Acα does not affect the proliferation of RGPs, it significantly impairs the generation of late-born neurons. We find complete loss of apical adherens junctions (AJs) in the ventricular membrane in Ppp2cα cKO cortices. We observe abundant colocalization for N-cadherin and PP2Acα in control AJs. Moreover, in vitro analysis reveals direct interactions of N-cadherin to PP2Acα and to β-catenin. Overall, this study not only uncovers a novel function of PP2Acα in retaining RGPs into the VZ but also demonstrates the impact of PP2A-dependent retention of RGPs on the generation for late-born neurons.

Sign in / Sign up

Export Citation Format

Share Document