Faculty Opinions recommendation of Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase.

2019 ◽  
Author(s):  
Zhihao Zhuang ◽  
Weijun Gui
Keyword(s):  
Genetic Code ◽  
Site Specific ◽  
Genetic Code Expansion

scholarly journals Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016)

2016 ◽  
Vol 55 (52) ◽  
pp. 15931-15931
Author(s):  
Ivana Nikić ◽  
Gemma Estrada Girona ◽  
Jun Hee Kang ◽  
Giulia Paci ◽  
Sofya Mikhaleva ◽  
...  
Keyword(s):  
Genetic Code ◽  
Super Resolution ◽  
Site Specific ◽  
Genetic Code Expansion ◽  
Super Resolution Microscopy

scholarly journals Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy

2016 ◽  
Vol 55 (52) ◽  
pp. 16172-16176 ◽  
Author(s):  
Ivana Nikić ◽  
Gemma Estrada Girona ◽  
Jun Hee Kang ◽  
Giulia Paci ◽  
Sofya Mikhaleva ◽  
...  
Keyword(s):  
Genetic Code ◽  
Super Resolution ◽  
Site Specific ◽  
Genetic Code Expansion ◽  
Super Resolution Microscopy

scholarly journals Bifunctional Non-Canonical Amino Acids: Combining Photo-Crosslinking with Click Chemistry

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 578 ◽  
Author(s):  
Jan-Erik Hoffmann
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Click Chemistry ◽  
Protein Interactions ◽  
Site Specific ◽  
Genetic Code Expansion

Genetic code expansion is a powerful tool for the study of protein interactions, as it allows for the site-specific incorporation of a photoreactive group via non-canonical amino acids. Recently, several groups have published bifunctional amino acids that carry a handle for click chemistry in addition to the photo-crosslinker. This allows for the specific labeling of crosslinked proteins and therefore the pulldown of peptides for further analysis. This review describes the properties and advantages of different bifunctional amino acids, and gives an overview about current and future applications.

Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase

2019 ◽  
Vol 15 (3) ◽  
pp. 276-284 ◽  
Author(s):  
Maximilian Fottner ◽  
Andreas-David Brunner ◽  
Verena Bittl ◽  
Daniel Horn-Ghetko ◽  
Alexander Jussupow ◽  
...  
Keyword(s):  
Genetic Code ◽  
Site Specific ◽  
Genetic Code Expansion

Synthesis of Site-Specific Radiolabeled Antibodies for Radioimmunotherapy via Genetic Code Expansion

2016 ◽  
Vol 27 (10) ◽  
pp. 2460-2468 ◽  
Author(s):  
Yiming Wu ◽  
Hua Zhu ◽  
Bo Zhang ◽  
Fei Liu ◽  
Jingxian Chen ◽  
...  
Keyword(s):  
Genetic Code ◽  
Site Specific ◽  
Genetic Code Expansion ◽  
Radiolabeled Antibodies

scholarly journals Pyrrolysyl-tRNA-Synthetase: Methanogenese und Gencode-Erweiterung

BIOspektrum ◽  
2021 ◽  
Vol 27 (6) ◽  
pp. 616-619
Author(s):  
Nikolaj Georg Koch ◽  
Nediljko Budisa
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Methanogenic Archaea ◽  
Trna Synthetase ◽  
Side Chains ◽  
Site Specific ◽  
Genetic Code Expansion ◽  
The Way

AbstractPyrrolysyl-tRNA synthetase (PylRS) is an enzyme of some methanogenic Archaea for the natural incorporation of pyrrolysine into proteins. The discovery of PylRS as a natural tool for genetic code expansion paved the way for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins, with versatile side chains useful in biotechnology. Almost 20 years after the discovery, we describe the journey which led to three distinct classes of PylRSs with unique ncAA recognitions.

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