Faculty Opinions recommendation of A Seed-Specific Regulator of Triterpene Saponin Biosynthesis in Medicago truncatula.

2020 ◽  
Author(s):  
Kazuki Saito
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis

scholarly journals The bHLH Transcription Factors TSAR1 and TSAR2 Regulate Triterpene Saponin Biosynthesis in Medicago truncatula

2015 ◽  
Vol 170 (1) ◽  
pp. 194-210 ◽  
Author(s):  
Jan Mertens ◽  
Jacob Pollier ◽  
Robin Vanden Bossche ◽  
Irene Lopez-Vidriero ◽  
José Manuel Franco-Zorrilla ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis ◽  
Bhlh Transcription Factors

scholarly journals Genomic and Coexpression Analyses Predict Multiple Genes Involved in Triterpene Saponin Biosynthesis in Medicago truncatula

2010 ◽  
Vol 22 (3) ◽  
pp. 850-866 ◽  
Author(s):  
Marina A. Naoumkina ◽  
Luzia V. Modolo ◽  
David V. Huhman ◽  
Ewa Urbanczyk-Wochniak ◽  
Yuhong Tang ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis ◽  
Multiple Genes

scholarly journals A genomics approach to the early stages of triterpene saponin biosynthesis in Medicago truncatula

2002 ◽  
Vol 32 (6) ◽  
pp. 1033-1048 ◽  
Author(s):  
Hideyuki Suzuki ◽  
Lahoucine Achnine ◽  
Ran Xu ◽  
Seiichi P. T. Matsuda ◽  
Richard A. Dixon
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Early Stages ◽  
Saponin Biosynthesis

scholarly journals A Seed-Specific Regulator of Triterpene Saponin Biosynthesis in Medicago truncatula

2020 ◽  
Vol 32 (6) ◽  
pp. 2020-2042 ◽  
Author(s):  
Bianca Ribeiro ◽  
Elia Lacchini ◽  
Keylla U. Bicalho ◽  
Jan Mertens ◽  
Philipp Arendt ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis

Enhanced triterpene saponin biosynthesis and root nodulation in transgenic barrel medic (Medicago truncatulaGaertn.) expressing a novel β-amyrin synthase (AsOXA1) gene

2009 ◽  
Vol 7 (2) ◽  
pp. 172-182 ◽  
Author(s):  
Massimo Confalonieri ◽  
Maria Cammareri ◽  
Elisa Biazzi ◽  
Paola Pecchia ◽  
Manuel Pedro Salema Fevereiro ◽  
...  
Keyword(s):  
Root Nodulation ◽  
Triterpene Saponin ◽  
Barrel Medic ◽  
Saponin Biosynthesis

scholarly journals CYP72A67 Catalyzes a Key Oxidative Step in Medicago truncatula Hemolytic Saponin Biosynthesis

2015 ◽  
Vol 8 (10) ◽  
pp. 1493-1506 ◽  
Author(s):  
Elisa Biazzi ◽  
Maria Carelli ◽  
Aldo Tava ◽  
Pamela Abbruscato ◽  
Ilaria Losini ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Saponin Biosynthesis

scholarly journals ER-Anchored Transcription Factors bZIP17 and bZIP60 Regulate Triterpene Saponin Biosynthesis in Medicago truncatula1[OPEN]

2020 ◽  
Author(s):  
Bianca Ribeiro ◽  
Marie-Laure Erffelinck ◽  
Maite Colinas ◽  
Clara Williams ◽  
Evelien Van Hamme ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Er Stress ◽  
Indole Alkaloid ◽  
Stress Conditions ◽  
Basic Leucine Zipper ◽  
Defense Compounds ◽  
Model Legume ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis ◽  
Bhlh Transcription Factors

ABSTRACTTriterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (JA). Two JA-modulated basic helix-loop-helix (bHLH) transcription factors (TFs), TRITERPENE SAPONIN BIOSYNTHESIS ACTIVATING REGULATOR 1 (TSAR1) and TSAR2, have been previously identified as direct activators of TS biosynthesis in the model legume Medicago truncatula. Here, we report on the involvement of the core endoplasmic reticulum (ER) stress basic leucine zipper (bZIP) TFs bZIP17 and bZIP60 in the regulation of TS biosynthesis. Expression and processing of M. truncatula bZIP17 and bZIP60 proteins was altered in roots with perturbed TS biosynthesis or treated with JA. Accordingly, such roots displayed an altered ER network structure. M. truncatula bZIP17 and bZIP60 proteins were shown to be capable of interfering with the TSAR-mediated transactivation of TS biosynthesis genes, particularly under ER stress conditions, when they translocate from the ER to the nucleus. Furthermore, the inhibitory role of ER stress bZIP TFs in the regulation of JA-dependent terpene biosynthetic pathways appears to be widespread in the plant kingdom, as we demonstrate that it also occurs in the regulation of monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus. We postulate that activation of ER stress bZIP TFs provides the plant with a mechanism to balance metabolic activities and thereby adequately govern modulation of growth, development and defense processes in defined stress situations.One sentence summaryER stress bZIP transcription factors can interfere with the activity of jasmonate-inducible bHLH transcription factors to modulate the elicitation of plant specialized metabolism in stress conditions.

scholarly journals CRISPR/Cas9-Mediated Targeted Mutagenesis of CYP93E2 Modulates the Triterpene Saponin Biosynthesis in Medicago truncatula

2021 ◽  
Vol 12 ◽  
Author(s):  
Massimo Confalonieri ◽  
Maria Carelli ◽  
Silvia Gianoglio ◽  
Andrea Moglia ◽  
Elisa Biazzi ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Metabolic Flux ◽  
Targeted Mutagenesis ◽  
In Planta ◽  
Knock Out ◽  
Mutant Plant ◽  
Guide Rnas ◽  
Saponin Biosynthesis ◽  
Pharmaceutical Properties ◽  
Adverse Influence

In the Medicago genus, triterpene saponins are a group of bioactive compounds extensively studied for their different biological and pharmaceutical properties. In this work, the CRISPR/Cas9-based approach with two single-site guide RNAs was used in Medicago truncatula (barrel medic) to knock-out the CYP93E2 and CYP72A61 genes, which are responsible for the biosynthesis of soyasapogenol B, the most abundant soyasapogenol in Medicago spp. No transgenic plants carrying mutations in the target CYP72A61 gene were recovered while fifty-two putative CYP93E2 mutant plant lines were obtained following Agrobacterium tumefaciens-mediated transformation. Among these, the fifty-one sequenced plant lines give an editing efficiency of 84%. Sequencing revealed that these lines had various mutation patterns at the target sites. Four T0 mutant plant lines were further selected and examined for their sapogenin content and plant growth performance under greenhouse conditions. The results showed that all tested CYP93E2 knock-out mutants did not produce soyasapogenols in the leaves, stems and roots, and diverted the metabolic flux toward the production of valuable hemolytic sapogenins. No adverse influence was observed on the plant morphological features of CYP93E2 mutants under greenhouse conditions. In addition, differential expression of saponin pathway genes was observed in CYP93E2 mutants in comparison to the control. Our results provide new and interesting insights into the application of CRISPR/Cas9 for metabolic engineering of high-value compounds of plant origin and will be useful to investigate the physiological functions of saponins in planta.

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