scholarly journals CYP72A67 Catalyzes a Key Oxidative Step in Medicago truncatula Hemolytic Saponin Biosynthesis

2015 ◽  
Vol 8 (10) ◽  
pp. 1493-1506 ◽  
Author(s):  
Elisa Biazzi ◽  
Maria Carelli ◽  
Aldo Tava ◽  
Pamela Abbruscato ◽  
Ilaria Losini ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Saponin Biosynthesis

scholarly journals The bHLH Transcription Factors TSAR1 and TSAR2 Regulate Triterpene Saponin Biosynthesis in Medicago truncatula

2015 ◽  
Vol 170 (1) ◽  
pp. 194-210 ◽  
Author(s):  
Jan Mertens ◽  
Jacob Pollier ◽  
Robin Vanden Bossche ◽  
Irene Lopez-Vidriero ◽  
José Manuel Franco-Zorrilla ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis ◽  
Bhlh Transcription Factors

scholarly journals Genomic and Coexpression Analyses Predict Multiple Genes Involved in Triterpene Saponin Biosynthesis in Medicago truncatula

2010 ◽  
Vol 22 (3) ◽  
pp. 850-866 ◽  
Author(s):  
Marina A. Naoumkina ◽  
Luzia V. Modolo ◽  
David V. Huhman ◽  
Ewa Urbanczyk-Wochniak ◽  
Yuhong Tang ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis ◽  
Multiple Genes

scholarly journals A genomics approach to the early stages of triterpene saponin biosynthesis in Medicago truncatula

2002 ◽  
Vol 32 (6) ◽  
pp. 1033-1048 ◽  
Author(s):  
Hideyuki Suzuki ◽  
Lahoucine Achnine ◽  
Ran Xu ◽  
Seiichi P. T. Matsuda ◽  
Richard A. Dixon
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Early Stages ◽  
Saponin Biosynthesis

scholarly journals CRISPR/Cas9-Mediated Targeted Mutagenesis of CYP93E2 Modulates the Triterpene Saponin Biosynthesis in Medicago truncatula

2021 ◽  
Vol 12 ◽  
Author(s):  
Massimo Confalonieri ◽  
Maria Carelli ◽  
Silvia Gianoglio ◽  
Andrea Moglia ◽  
Elisa Biazzi ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Metabolic Flux ◽  
Targeted Mutagenesis ◽  
In Planta ◽  
Knock Out ◽  
Mutant Plant ◽  
Guide Rnas ◽  
Saponin Biosynthesis ◽  
Pharmaceutical Properties ◽  
Adverse Influence

In the Medicago genus, triterpene saponins are a group of bioactive compounds extensively studied for their different biological and pharmaceutical properties. In this work, the CRISPR/Cas9-based approach with two single-site guide RNAs was used in Medicago truncatula (barrel medic) to knock-out the CYP93E2 and CYP72A61 genes, which are responsible for the biosynthesis of soyasapogenol B, the most abundant soyasapogenol in Medicago spp. No transgenic plants carrying mutations in the target CYP72A61 gene were recovered while fifty-two putative CYP93E2 mutant plant lines were obtained following Agrobacterium tumefaciens-mediated transformation. Among these, the fifty-one sequenced plant lines give an editing efficiency of 84%. Sequencing revealed that these lines had various mutation patterns at the target sites. Four T0 mutant plant lines were further selected and examined for their sapogenin content and plant growth performance under greenhouse conditions. The results showed that all tested CYP93E2 knock-out mutants did not produce soyasapogenols in the leaves, stems and roots, and diverted the metabolic flux toward the production of valuable hemolytic sapogenins. No adverse influence was observed on the plant morphological features of CYP93E2 mutants under greenhouse conditions. In addition, differential expression of saponin pathway genes was observed in CYP93E2 mutants in comparison to the control. Our results provide new and interesting insights into the application of CRISPR/Cas9 for metabolic engineering of high-value compounds of plant origin and will be useful to investigate the physiological functions of saponins in planta.

scholarly journals A Seed-Specific Regulator of Triterpene Saponin Biosynthesis in Medicago truncatula

2020 ◽  
Vol 32 (6) ◽  
pp. 2020-2042 ◽  
Author(s):  
Bianca Ribeiro ◽  
Elia Lacchini ◽  
Keylla U. Bicalho ◽  
Jan Mertens ◽  
Philipp Arendt ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis
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