scholarly journals Faculty Opinions recommendation of The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans.

2020 ◽  
Author(s):  
Douglas Allen
Keyword(s):  
Desulfovibrio Desulfuricans ◽  
Autotrophic Growth

scholarly journals The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Irene Sánchez-Andrea ◽  
Iame Alves Guedes ◽  
Bastian Hornung ◽  
Sjef Boeren ◽  
Christopher E. Lawson ◽  
...  
Keyword(s):  
Growth Rate ◽  
Desulfovibrio Desulfuricans ◽  
Ammonia Concentration ◽  
Autotrophic Growth ◽  
Acetyl Coa ◽  
Energy Substrates ◽  
Glycine Reductase

Abstract Six CO2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO2 via the reductive glycine pathway, a seventh CO2 fixation pathway. In this pathway, CO2 is first reduced to formate, which is reduced and condensed with a second CO2 to generate glycine. Glycine is further reduced in D. desulfuricans by glycine reductase to acetyl-P, and then to acetyl-CoA, which is condensed with another CO2 to form pyruvate. Ammonia is involved in the operation of the pathway, which is reflected in the dependence of the autotrophic growth rate on the ammonia concentration. Our study demonstrates microbial autotrophic growth fully supported by this highly ATP-efficient CO2 fixation pathway.

scholarly journals Enhanced hydrogenation catalyst synthesized by Desulfovibrio desulfuricans exposed to a radio frequency magnetic field

2021 ◽  
Author(s):  
Lynne E. Macaskie ◽  
John Collins ◽  
Iryna P. Mikheenko ◽  
Jaime Gomez‐Bolivar ◽  
Mohamed L. Merroun ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Radio Frequency ◽  
Desulfovibrio Desulfuricans ◽  
Hydrogenation Catalyst ◽  
Frequency Magnetic Field

scholarly journals Metabolic engineering of Moorella thermoacetica for thermophilic bioconversion of gaseous substrates to a volatile chemical

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Junya Kato ◽  
Kaisei Takemura ◽  
Setsu Kato ◽  
Tatsuya Fujii ◽  
Keisuke Wada ◽  
...  
Keyword(s):  
Carbon Flux ◽  
Cost Effective ◽  
Gas Composition ◽  
Autotrophic Growth ◽  
Acetyl Coenzyme A ◽  
Volatile Chemicals ◽  
Moorella Thermoacetica ◽  
Gas Fermentation ◽  
Dry Cell ◽  
Co Gas

AbstractGas fermentation is one of the promising bioprocesses to convert CO2 or syngas to important chemicals. Thermophilic gas fermentation of volatile chemicals has the potential for the development of consolidated bioprocesses that can simultaneously separate products during fermentation. This study reports the production of acetone from CO2 and H2, CO, or syngas by introducing the acetone production pathway using acetyl–coenzyme A (Ac-CoA) and acetate produced via the Wood–Ljungdahl pathway in Moorella thermoacetica. Reducing the carbon flux from Ac-CoA to acetate through genetic engineering successfully enhanced acetone productivity, which varied on the basis of the gas composition. The highest acetone productivity was obtained with CO–H2, while autotrophic growth collapsed with CO2–H2. By adding H2 to CO, the acetone productivity from the same amount of carbon source increased compared to CO gas only, and the maximum specific acetone production rate also increased from 0.04 to 0.09 g-acetone/g-dry cell/h. Our development of the engineered thermophilic acetogen M. thermoacetica, which grows at a temperature higher than the boiling point of acetone (58 °C), would pave the way for developing a consolidated process with simplified and cost-effective recovery via condensation following gas fermentation.

scholarly journals Amino acid sequence of the [4Fe-4S] ferredoxin isolated from Desulfovibrio desulfuricans Norway.

1985 ◽  
Vol 260 (14) ◽  
pp. 8292-8296 ◽  
Author(s):  
M H Bruschi ◽  
F A Guerlesquin ◽  
G E Bovier-Lapierre ◽  
J J Bonicel ◽  
P M Couchoud
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Desulfovibrio Desulfuricans

scholarly journals Metagenomics-guided analysis of microbial chemolithoautotrophic phosphite oxidation yields evidence of a seventh natural CO2fixation pathway

2017 ◽  
Vol 115 (1) ◽  
pp. E92-E101 ◽  
Author(s):  
Israel A. Figueroa ◽  
Tyler P. Barnum ◽  
Pranav Y. Somasekhar ◽  
Charlotte I. Carlström ◽  
Anna L. Engelbrektson ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
Carbon Fixation ◽  
Community Analysis ◽  
Metabolic Model ◽  
Rrna Gene ◽  
Autotrophic Growth ◽  
Anaerobic Wastewater Treatment ◽  
Natural Carbon ◽  
Environmental Relevance

Dissimilatory phosphite oxidation (DPO), a microbial metabolism by which phosphite (HPO32−) is oxidized to phosphate (PO43−), is the most energetically favorable chemotrophic electron-donating process known. Only one DPO organism has been described to date, and little is known about the environmental relevance of this metabolism. In this study, we used 16S rRNA gene community analysis and genome-resolved metagenomics to characterize anaerobic wastewater treatment sludge enrichments performing DPO coupled to CO2reduction. We identified an uncultivated DPO bacterium,CandidatusPhosphitivorax (Ca.P.) anaerolimi strain Phox-21, that belongs to candidate order GW-28 within theDeltaproteobacteria, which has no known cultured isolates. Genes for phosphite oxidation and for CO2reduction to formate were found in the genome ofCa.P. anaerolimi, but it appears to lack any of the known natural carbon fixation pathways. These observations led us to propose a metabolic model for autotrophic growth byCa.P. anaerolimi whereby DPO drives CO2reduction to formate, which is then assimilated into biomass via the reductive glycine pathway.

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