Faculty Opinions recommendation of Genomic landscape of single-stranded DNA gapped intermediates in Escherichia coli.

2021 ◽  
Author(s):  
Christian Rudolph
Keyword(s):  
Escherichia Coli ◽  
Single Stranded Dna ◽  
Genomic Landscape

scholarly journals msDNA-Ec48, the smallest multicopy single-stranded DNA from Escherichia coli.

1997 ◽  
Vol 179 (24) ◽  
pp. 7865-7868 ◽  
Author(s):  
J R Mao ◽  
S Inouye ◽  
M Inouye
Keyword(s):  
Escherichia Coli ◽  
Single Stranded Dna

Properties of the high-affinity single-stranded DNA binding state of the Escherichia coli recA protein

Biochemistry ◽  
1988 ◽  
Vol 27 (4) ◽  
pp. 1205-1212 ◽  
Author(s):  
Joseph P. Menetski ◽  
Abraham Varghese ◽  
Stephen C. Kowalczykowski
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Reca Protein ◽  
Single Stranded Dna ◽  
High Affinity

scholarly journals F sex factor of Escherichia coli K-12 codes for a single-stranded DNA binding protein.

1983 ◽  
Vol 80 (14) ◽  
pp. 4422-4426 ◽  
Author(s):  
A. L. Kolodkin ◽  
M. A. Capage ◽  
E. I. Golub ◽  
K. B. Low
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Stranded Dna ◽  
K 12

scholarly journals Effects of recJ, recQ, and recFOR Mutations on Recombination in Nuclease-Deficient recB recD Double Mutants of Escherichia coli

2005 ◽  
Vol 187 (4) ◽  
pp. 1350-1356 ◽  
Author(s):  
Ivana Ivančić-Baće ◽  
Erika Salaj-Šmic ◽  
Krunoslav Brčić-Kostić
Keyword(s):  
Escherichia Coli ◽  
Double Mutant ◽  
Reca Protein ◽  
Recq Helicase ◽  
Single Stranded Dna ◽  
Double Stranded Dna ◽  
Recf Pathway ◽  
Recombination Pathway ◽  
Recbcd Enzyme ◽  
Dna Ends

ABSTRACT The two main recombination pathways in Escherichia coli (RecBCD and RecF) have different recombination machineries that act independently in the initiation of recombination. Three essential enzymatic activities are required for early recombinational processing of double-stranded DNA ends and breaks: a helicase, a 5′→3′ exonuclease, and loading of RecA protein onto single-stranded DNA tails. The RecBCD enzyme performs all of these activities, whereas the recombination machinery of the RecF pathway consists of RecQ (helicase), RecJ (5′→3′ exonuclease), and RecFOR (RecA-single-stranded DNA filament formation). The recombination pathway operating in recB (nuclease-deficient) mutants is a hybrid because it includes elements of both the RecBCD and RecF recombination machineries. In this study, genetic analysis of recombination in a recB (nuclease-deficient) recD double mutant was performed. We show that conjugational recombination and DNA repair after UV and gamma irradiation in this mutant are highly dependent on recJ, partially dependent on recFOR, and independent of recQ. These results suggest that the recombination pathway operating in a nuclease-deficient recB recD double mutant is also a hybrid. We propose that the helicase and RecA loading activities belong to the RecBCD recombination machinery, while the RecJ-mediated 5′→3′ exonuclease is an element of the RecF recombination machinery.

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