scholarly journals Vetores de adenovírus na terapia gênica: Uma revisão sobre sua aplicabilidade e perspectivas / Adenovírus vectors in gene therapy: A review of its applicability and perspectives

2021 ◽  
Vol 7 (12) ◽  
pp. 114428-114440
Author(s):  
Bianca Pereira Nascimento ◽  
João José De Deus Costa Carneiro ◽  
Dr. Pedro Rauel Cândido Domingos
Keyword(s):  
Gene Therapy ◽  
Terapia Génica ◽  
Adenovirus Vectors

Gene therapy with recombinant adenovirus vectors: evaluation of the host immune response

1997 ◽  
Vol 57 (1-3) ◽  
pp. 19-25 ◽  
Author(s):  
Marielle Christ ◽  
Monika Lusky ◽  
Fabienne Stoeckel ◽  
Dominique Dreyer ◽  
Annick Dieterlé ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Immune Response ◽  
Recombinant Adenovirus ◽  
Host Immune Response ◽  
Adenovirus Vectors

Gene therapy: adenovirus vectors

1993 ◽  
Vol 3 (3) ◽  
pp. 499-503 ◽  
Author(s):  
Karen F. Kozarsky ◽  
James M. Wilson
Keyword(s):  
Gene Therapy ◽  
Adenovirus Vectors

scholarly journals Activation of p38 and ERK Signaling during Adenovirus Vector Cell Entry Lead to Expression of the C-X-C Chemokine IP-10

2002 ◽  
Vol 76 (4) ◽  
pp. 1559-1568 ◽  
Author(s):  
Lee Anne Tibbles ◽  
Jason C. L. Spurrell ◽  
Gloria P. Bowen ◽  
Qiang Liu ◽  
Mindy Lam ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Mrna Expression ◽  
Inflammatory Responses ◽  
Human Gene ◽  
Adenovirus Vector ◽  
Endosomal Escape ◽  
Human Gene Therapy ◽  
Serotype 5 ◽  
Adenovirus Vectors

ABSTRACT The use of adenovirus vectors for human gene therapy is limited by potent inflammatory responses that result in significant morbidity. In kidney-derived epithelial cells (REC), activation of extracellular signal-regulated kinase 1/2 (ERK) and p38 kinase (p38) pathways occurred within 20 min of transduction with the serotype 5 adenovirus vector AdCMVβgal. Inhibition of ERK and p38 with U0126 and SB203580, respectively, reduced the expression of IP-10 mRNA following transduction with AdCMVβgal. To determine the role of the coxsackievirus-adenovirus receptor (CAR) or αv integrins in the activation of ERK and p38 and the expression of IP-10, REC cells were transduced with the fiber-modified and RGD-deleted adenovirus vectors AdL.F(RAEK-HA) and AdL.PB(HA), respectively. Compared with the wild-type capsid vector Ad5Luc, transduction with AdL.F(RAEK-HA) and AdL.PB(HA) resulted in reduced ERK-p38 activation and less IP-10 mRNA expression. The decreased IP-10 expression induced by the tropism-modified vectors was due to diminished transduction, since increasing multiplicity of infection resulted in increased IP-10 expression. Inhibition of adenovirus penetration with bafilomycin A1 or ammonium chloride attenuated the activation of ERK-p38 and IP-10 mRNA expression following infection, suggesting that endosomal escape was required to trigger these pathways. In vivo, direct inhibition of ERK and p38 signaling pathways inhibited adenovirus vector-induced IP-10 expression in mouse liver 1 h following transduction. These results demonstrate the importance of signaling via ERK and p38 in the early host response to adenovirus vectors and will permit the development of novel strategies to improve the safety and efficacy of these agents in human gene therapy.

Adenovirus Vectors Based on Human Adenovirus Type 19a Have High Potential for Human Muscle-Directed Gene Therapy

2006 ◽  
Vol 17 (2) ◽  
pp. 193-205 ◽  
Author(s):  
Christian Thirion ◽  
Hanns Lochmüller ◽  
Zsolt Ruzsics ◽  
Marc Boelhauve ◽  
Cornelia König ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Human Muscle ◽  
High Potential ◽  
Adenovirus Vectors
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