scholarly journals Expression, purification and characterization of anti-FGF7 domain antibody identified using phage display technique

2021 ◽  
Author(s):  
Aliakbar Alizadeh ◽  
Mona Roshani ◽  
Omid Jamshidi Kandjani ◽  
Milad Soltani-Saif ◽  
Siavoush Dastmalchi
Keyword(s):  
Phage Display ◽  
Md Simulations ◽  
Docking Study ◽  
Single Domain Antibody ◽  
Cancer Cell Growth ◽  
Phage Display Technique ◽  
Domain Antibody ◽  
Sds Page ◽  
Display Technique

Background: Fibroblast growth factors (FGFs) are involved in angiogenesis, wound healing and embryonic development. However, one of the causes of cancer cell growth in fibroblast-dependent cancers is FGF7 secreted by fibroblasts. Therefore, antibodies against FGF7 can be used for treatment of these types of cancers. Methods: In previous studies, a phage displaying single domain antibody, D53, against human FGF7 has been identified using the phage display technique. In the present study, D53 was produced and purified in its isolated form. ELISA experiment was performed to evaluate the binding of D53 to FGF7. The mode of interaction of D53-FGF7 was explored using docking study and molecular dynamics (MD) simulations. Results: The expression and purification processes were verified using western blotting and SDS-PAGE analyses. ELISA experiment showed that D53 is able to recognize and bind FGF7. Docking study and MD simulations indicated that compared to dummy VH, D53 has more affinity towards FGF7. Conclusion: The findings in the current study can be useful for generation and development of FGF7 inhibitors with potential use in fibroblast-dependent cancers.

Isolation and characterization of anti ROR1 single chain fragment variable antibodies using phage display technique

2017 ◽  
Vol 25 (1-2) ◽  
pp. 57-63 ◽  
Author(s):  
Leili Aghebati-Maleki ◽  
Vahid Younesi ◽  
Farhad Jadidi-Niaragh ◽  
Behzad Baradaran ◽  
Jafar Majidi ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Chain Fragment ◽  
Phage Display Technique ◽  
Isolation And Characterization ◽  
Single Chain Fragment Variable ◽  
Display Technique ◽  
Single Chain Fragment

Production, characterization and in-vitro applications of single-domain antibody against thyroglobulin selected from novel T7 phage display library

2021 ◽  
Vol 492 ◽  
pp. 112990
Author(s):  
Jothivel Kumarasamy ◽  
Samar Kumar Ghorui ◽  
Chandrakala Gholve ◽  
Bharti Jain ◽  
Yogesh Dhekale ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Display Library ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
T7 Phage Display ◽  
T7 Phage

Development of Human Antibody Inhibiting RNase H Activity of Polymerase of Hepatitis B Virus Using Phage Display Technique

2004 ◽  
Vol 4 (1) ◽  
pp. 16
Author(s):  
Seong-Rak Lee ◽  
Eun-Kyoung Song ◽  
Young-Joo Jeong ◽  
Young-Yi Lee ◽  
Ik-Jung Kim ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Phage Display ◽  
Rnase H ◽  
Human Antibody ◽  
Phage Display Technique ◽  
B Virus ◽  
Display Technique

Phage-based Nanomedicines as New Immune Therapeutic Agents for Breast Cancer

2018 ◽  
Vol 24 (11) ◽  
pp. 1195-1203 ◽  
Author(s):  
Atefeh Arab ◽  
Rezvan Yazdian Robati ◽  
Jessica Nicastro ◽  
Roderick Slavcev ◽  
Javad Behravan
Keyword(s):  
Breast Cancer ◽  
Phage Display ◽  
Biomedical Applications ◽  
Therapeutic Agents ◽  
Molecular Method ◽  
Specific Antibodies ◽  
Phage Display Technique ◽  
Specific Antigens ◽  
Protein Libraries ◽  
Display Technique

Despite years of investigation, breast cancer remains a major cause of death worldwide. Phage display is a powerful molecular method in which peptide and protein libraries can be displayed via genetic fusions on the surface of phages. This approach has tremendous potential for biomedical applications and has already facilitated the discovery of specific antibodies, specific antigens, and peptides with potential roles in the diagnosis and treatment of malignancies including breast cancer. In this review, we discuss the new and the latest advancements in the applications of the phage display technique in the provision of immune therapeutics for breast cancer.

scholarly journals Use of a Phage Display Technique to Identify Potential Osteoblast Binding Sites Within Osteoclast Lacunae

2002 ◽  
Vol 17 (5) ◽  
pp. 915-922 ◽  
Author(s):  
Tzong-Jen Sheu ◽  
Edward M. Schwarz ◽  
Regis J. O'keefe ◽  
Randy N. Rosier ◽  
J. Edward Puzas
Keyword(s):  
Phage Display ◽  
Binding Sites ◽  
Phage Display Technique ◽  
Display Technique

Exploration of Peptide Inhibitors of Human Squalene Synthase through Molecular Modeling and Phage Display Technique

2015 ◽  
Vol 178 (2) ◽  
pp. 312-323 ◽  
Author(s):  
David Shiuan ◽  
Hwan-Kang Lin ◽  
Yue-Hao Chen ◽  
Ding-Kwo Chang ◽  
Kao-Jean Huang ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Phage Display ◽  
Peptide Inhibitors ◽  
Squalene Synthase ◽  
Phage Display Technique ◽  
Human Squalene Synthase ◽  
Display Technique

scholarly journals Next-Generation Sequencing of a Single Domain Antibody Repertoire Reveals Quality of Phage Display Selected Candidates

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0149393 ◽  
Author(s):  
Kendrick B. Turner ◽  
Jennifer Naciri ◽  
Jinny L. Liu ◽  
George P. Anderson ◽  
Ellen R. Goldman ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Phage Display ◽  
Single Domain ◽  
Antibody Repertoire ◽  
Next Generation ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Generation Sequencing
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