Ultrasound biomicroscopic comparison of primary open-angle glaucoma and primary angle-closure glaucoma eyes in dark and light conditions

Background: With the use of ultrasound biomicroscopy, we aim to look at differences in anterior segment parameters of eyes with primary open angle glaucoma (POAG) and primary angle closure glaucoma (PACG) in dark and light conditions.Methods: Ultrasound biomicroscopy was performed for 30 subjects with PACG and 30 subjects with POAG at initial presentation before any treatment. Measurements of angle opening distance (AOD-500 and AOD-750) and trabecular-iris space area (TISA-500 and TISA-750) 500 and 750 mm from the scleral spur in both dark and light conditions were made. Anterior chamber depth (ACD) and axial length (AL) were also measured.Results: The mean age of PACG patients was 67.6 ± 9.6 years and POAG patients 62.1 ± 13.9 years. The mean ACD (2.70 ± 0.53mm) in PACG patients was significantly different from that (3.32 ± 0.52mm) of POAG patients (p<0.0001). There were also significant differences (p=0.0004) in the mean AL of PACG (22.91 ± 0.86mm) and POAG (24.47 ± 1.67mm) patients. Significant differences between POAG and PACG eyes were found for TISA-500, TISA-750, AOD-500 and AOD-750 in both light and dark conditions (p<0.001 for all). The light-dark differences in PACG eyes were smaller than that of POAG eyes for all AOD and TISA values in the inferior, superior, nasal and temporal quadrants. However, with the exception of AOD-750 in the inferior quadrant (p=0.0524), there were no significant differences in light-dark changes between POAG and PACG eyes for all parameters in the 4 quadrants.Conclusions: Ultrasound biomicroscopy is a useful tool in the diagnosis and management of glaucoma. We found significant differences in mean AL, ACD, TISA-500, TISA-750, AOD-500 and AOD-750 between PACG and POAG eyes. However, there were no significant differences between PACG and POAG eyes in terms of light-dark difference in anterior segment parameters, except for AOD-750 in the inferior quadrant. Further evaluation of the above findings could be done in future with a larger population.

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Biallelic variants in CPAMD8 are associated with primary open-angle glaucoma and primary angle-closure glaucoma

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2020-318668 ◽

2021 ◽

pp. bjophthalmol-2020-318668

Author(s):

Xueqing Li ◽

Wenmin Sun ◽

Xueshan Xiao ◽

Lei Fang ◽

Shiqiang Li ◽

...

Keyword(s):

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Anterior Segment ◽

Angle Closure Glaucoma ◽

Congenital Glaucoma ◽

Angle Closure ◽

Open Angle ◽

Ciliary Process ◽

Primary Angle Closure ◽

Primary Angle Closure Glaucoma

Background/AimsThis study aims to assess the contribution of biallelic CPAMD8 variants in patients with different forms of glaucoma, especially primary open-angle glaucoma (POAG) and primary angle-closure glaucoma (PACG), based on a systematic analysis of exome sequencing (ES).MethodsPotentially pathogenic CPAMD8 variants were selected from the ES data of 5307 subjects with various eye conditions through multiple bioinformatics analyses. Of the 5307 subjects, 1221 probands had different forms of primary glaucoma. The genotype–phenotype correlation was assessed by a systematic review of biallelic CPAMD8 variants that including our data and data from the literature. The expression profile of CPAMD8 in human tissues was determined at the mRNA and protein levels.ResultsBiallelic CPAMD8 variants, including one frameshift and six missense variants, were exclusively present and significantly enriched in patients with glaucoma (one with juvenile open-angle glaucoma (JOAG), two with POAG and two with PACG) compared with none of the 4086 probands with other eye conditions in this cohort (p=4.1E-07). The effect of variants in these patients is relatively mild compared with that reported in patients with anterior segment dysgenesis or primary congenital glaucoma. CPAMD8 mRNA was highly expressed in the optic nerve, ciliary body, retina and iris, whereas the CPAMD8 protein was mainly detected in the nonpigmented epithelium of the iris and ciliary process, determined by immunohistochemistry.ConclusionsThe data from this study not only provide further evidence to support the association of biallelic CPAMD8 variants with JOAG but also suggest that biallelic CPAMD8 variants might be associated with POAG and PACG.

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