Simultaneous detection of Epstein - Barr virus and Cytomegalovirus DNA in Mini-pooling of Whole blood Samples from Iraqi blood donors by Multiplex Real-Time PCR Assay

2020 ◽  
Vol 23 (14) ◽  
Author(s):  
Ghinwa S. Majid ◽  
Ahmed S. Abdulamir ◽  
Abbas M. Ahmed ◽  
Iman M. Aufi ◽  
Orooba I. Abdullah
Keyword(s):  
Real Time Pcr ◽  
Whole Blood ◽  
Epstein Barr Virus ◽  
Blood Donors ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Blood Samples ◽  
Barr Virus ◽  
Whole Blood Samples ◽  
Epstein Barr

Quantitative Analysis of Epstein-Barr Virus Load by Using a Real-Time PCR Assay

1999 ◽  
Vol 37 (1) ◽  
pp. 132-136 ◽  
Author(s):  
Hiroshi Kimura ◽  
Makoto Morita ◽  
Yumi Yabuta ◽  
Kiyotaka Kuzushima ◽  
Koji Kato ◽  
...  
Keyword(s):  
Real Time ◽  
Infectious Mononucleosis ◽  
Real Time Pcr ◽  
Copy Number ◽  
Epstein Barr Virus ◽  
Pcr Assay ◽  
Virus Load ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 107 copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 103.7 copies/μg of DNA in patients with EBV-related lymphoproliferative disorders, 104.1 copies/μg of DNA in patients with chronic active EBV infections, and 102.2copies/μg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.

Evaluation of an automated and highly sensitive real-time PCR assay for CMV DNA in whole blood samples

2006 ◽  
Vol 36 ◽  
pp. S26
Author(s):  
C. Deback ◽  
S. Akhavan ◽  
S. Blanc-Perrel ◽  
F. Dupuis ◽  
S. Schaffer ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Pcr Assay ◽  
Blood Samples ◽  
Highly Sensitive ◽  
Whole Blood Samples

A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples

2007 ◽  
Vol 197 (3) ◽  
pp. 313-324 ◽  
Author(s):  
Lutz Eric Lehmann ◽  
Klaus-Peter Hunfeld ◽  
Thomas Emrich ◽  
Gerd Haberhausen ◽  
Heimo Wissing ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Rapid Detection ◽  
Fungal Pathogens ◽  
Pcr Assay ◽  
Blood Samples ◽  
Whole Blood Samples
Sign in / Sign up

Export Citation Format

Share Document