scholarly journals Methods of inoculation of potato tubers when assessing resistance to Phoma exigua

Author(s):  
T.O. Andriychuk ◽  
A.M. Skoreiko ◽  
O.M. Nemchenko ◽  
A.T. Melnik

The results of studies on methods of inoculation of potato tubers when assessing resistance to Phoma exigua. For this is suitable both ways tuber infection, making inokulumu (agar blocks) in the hole and sinking bubbles in the suspension of mycelium fungus.

2020 ◽  
Vol 21 (4) ◽  
pp. 335-337
Author(s):  
James W. Woodhall ◽  
Lara Brown ◽  
Miranda Harrington ◽  
Nora Olsen ◽  
Jeff Miller ◽  
...  

Potato tubers (cultivar ‘Austrian Crescent’) displaying a dry rot decay symptom and internal sclerotia were received for diagnosis. Isolations were attempted from symptomatic material, and Sclerotinia sclerotiorum was frequently recovered. Pathogenicity tests for S. sclerotiorum in potato tubers were completed using agar plugs with S. sclerotiorum placed over tuber wounds and through injecting stolon end with a S. sclerotiorum hyphal suspension. Both inoculation methods induced decay symptoms, although these were observed less frequently with the agar plug inoculation (37.5%) compared with hyphal injection (100%). S. sclerotiorum was consistently reisolated from inoculated symptomatic tubers. This symptom is rare, but potato growers should be aware that tuber infection is possible when white mold is present.


2014 ◽  
Vol 54 (3) ◽  
pp. 225-230
Author(s):  
Katarzyna Rymuza ◽  
Antoni Bombik ◽  
Dariusz Stopa ◽  
Zbigniew Pawlonka

Abstract A potato experiment was conducted from 2004 to 2006 at the Zawady Experimental Farm. The farm is part of the University of Natural Sciences and Humanities in Siedlce, Poland. Tuber infection and injury by diseases and pathogens of three potato cultivars were assessed according to the potato ridge height and harvest date. The weight of mechanically injured tubers was also determined depending on the potato ridge height and harvest date. The weight of potato tubers infected by fungal and bacterial diseases was influenced by the cultivar, growing season, harvest date, and ridge height. Potatoes grown to their standard height and height ridges, were significantly less infected by diseases at the first harvest date. The weight of tubers in which the tubers were injured by soil pests, was affected by the cultivar and ridge height. Potatoes cultivated in a ridge which was 20 cm high, had tubers with significantly less mechanical injuries compared with a low (16 cm) ridge and high (24 cm) ridge.


1976 ◽  
Vol 24 (3) ◽  
pp. 337 ◽  
Author(s):  
RR Walker ◽  
GC Wade

In Tasmania, Phoma exigua Desm. var. exigua accounts for 90% of potential inoculum on tuber surfaces and 90% of gangrene lesions in stored potato tubers. P. exigua Desm, var, foveata (Foister) Boerema accounts for the remaining 10%. Tubers lifted from pre-senescent crops contain negligible levels of surface-borne inoculum and develop few lesions in storage. Washing removes only a small percentage of potential inoculum on tuber surfaces, which initiates infections if tubers are wounded and held in an environment unfavourable for wound healing. Tuber resistance to gangrene disease is determined by the interaction between tuber cultivar, tuber maturity and the nature of the fungal isolate within both varieties of P. exigua. The resistance response is greatest at high temperatures and is characterized by three phases: lesion retardation, lesion arrest and lesion rejection. Lesion rejection represents the end product of tuber resistance response and is associated with the development of post-infectional periderm.


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