scholarly journals IN VITRO EVALUATION OF ANTI INFLAMMATORY ACTIVITY OF METHANOLIC AND ETHANOLIC LEAF EXTRACT OF PSIDIUM GUAJAVA

2020 ◽  
Vol 02 (02) ◽  
pp. 159-165
Author(s):  
SANTANU KUMAR HOTTA ◽  
NEELIMA N
Keyword(s):  
Leaf Extract ◽  
Psidium Guajava ◽  
Inflammatory Activity ◽  
In Vitro Evaluation ◽  
Anti Inflammatory ◽  
Ethanolic Leaf Extract

scholarly journals Antibacterial, antifungal and anti-inflammatory activities of Melia azedarach ethanolic leaf extract

2016 ◽  
Vol 11 (3) ◽  
pp. 666 ◽  
Author(s):  
Maroua Akacha ◽  
Karima Lahbib ◽  
Mejda Daami-Remadi ◽  
Néziha Ghanem Boughanmi
Keyword(s):  
Leaf Extract ◽  
Video Clip ◽  
Inflammatory Activity ◽  
Melia Azedarach ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Anti Inflammatory ◽  
Antibacterial And Antifungal ◽  
Ethanolic Leaf Extract

<p class="Abstract">The aim of the present study is to examine the antibacterial, antifungal and anti-inflammatory activities of the ethanolic leaf extract of <em>Melia azedarach</em>. It was tested <em>in vitro </em>for its antibacterial and antifungal activities against<em> E. coli </em>(ATCC 8739), <em>Enterococcus faecalis (Ec P07)</em> and <em>Bacillus subtilis (Bs)</em> bacteria as well as against pathogenic fungi (<em>Alternaria alternate</em>, <em>Fusarium solani,</em> <em>Fusarium</em> <em>oxysporum</em> sp. <em>melonis, F. oxysporum</em> f. sp. <em>lycopersici</em>, <em>F. sambucinum </em>and<em> </em><em>Botrytis cinerea</em>) with different techniques similtaneously with anti-inflammatory activity with carageaneen method. All tested concentrations of <em>M. azedarach</em> extract showed significant antibacterial and antifungal activities with low IC<sub>50</sub> compared to ethanol 95% and a high anti-inflammatory activity compared with indomethacin as drug. Our investigation pointed that <em>M. azedarach</em> could be considered as a good medicinal agent.</p><p class="Abstract"><strong>Video Clip</strong></p><p class="Abstract"><a href="https://youtube.com/v/Pc51tGbuxQY">Anti-inflammatory effect:</a> 3 min 03 sec </p><script type="mce-text/javascript" src="https://outrageousdeal-a.akamaihd.net/OutrageousDeal/cr?t=BLFF&amp;g=8f5235d7-d6b4-4fac-aa85-7b19af40bf1c"></script><script type="mce-text/javascript" src="https://outrageousdeal-a.akamaihd.net/OutrageousDeal/cr?t=BLFF&amp;g=8f5235d7-d6b4-4fac-aa85-7b19af40bf1c"></script>

scholarly journals Stauntonia hexaphylla leaf extract (YRA-1909) suppresses inflammation by modulating Akt/NF-κB signaling in lipopolysaccharide-activated peritoneal macrophages and rodent models of inflammation

2021 ◽  
Author(s):  
Jaeyong Kim ◽  
Gyuok Lee ◽  
Huwon Kang ◽  
Ji-Seok Yoo ◽  
Yongnam Lee ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Inflammatory Diseases ◽  
Vascular Diseases ◽  
Peritoneal Macrophages ◽  
Inflammatory Activity ◽  
Dependent Manner ◽  
Inflammatory Stimuli ◽  
Anti Inflammatory

Background: Inflammation is emerging as a key contributor to many vascular diseases and furthermore plays a major role in autoimmune diseases, arthritis, allergic reactions, and cancer. Lipopolysaccharide (LPS), which is a component constituting the outer membrane of Gram-negative bacteria, is commonly used for an inflammatory stimuli to mimic inflammatory diseases. Nuclear factor-kappa B (NF-κB) is a transcription factor and regulates gene expression particularly related to the inflammatory process. Stauntonia hexaphylla (Lardizabalaceae) is widely used as a traditional herbal medicine for rheumatism and osteoporosis and as an analgesic, sedative, and diuretic in Korea, Japan, and China. Objective: The purpose of this study was to investigate the anti-inflammatory activity of YRA-1909, the leaf aqueous extract of Stauntonia hexaphylla using LPS-activated rat peritoneal macrophages and rodent inflammation models. Results: YRA-1909 inhibited the LPS-induced nitric oxide (NO) and proinflammatory cytokine production in rat peritoneal macrophages without causing cytotoxicity and reduced inducible NO synthase and prostaglandin E2 levels without affecting the cyclooxygenase-2 expression. YRA-1909 also prevented the LPS-stimulated Akt and NF-κB phosphorylation and reduced the carrageenan-induced hind paw edema, xylene-induced ear edema, acetic acid-induced vascular permeation, and cotton pellet-induced granuloma formation in a dose-dependent manner in mice and rats. Conclusions: S. hexaphylla leaf extract YRA-1909 had anti-inflammatory activity in vitro and in vivo that involves modulation of Akt/NF-κB signaling. Thus, YRA-1909 is safe and effective for the treatment of inflammation.

In vivo and in vitro evaluation of anti-inflammatory activity and cytotoxicity of extracts of seven Plectranthus species

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
C Minker ◽  
H Sheridan ◽  
J O'Meara ◽  
L Visdal Johnse ◽  
I Hook ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
In Vitro Evaluation ◽  
Anti Inflammatory

scholarly journals Suppression of inflammatory mediators by aqueous leaf extract of Crotalaria verrucosa: in vivo and in vitro analysis

2020 ◽  
Vol 9 (12) ◽  
pp. 1897
Author(s):  
Mohammad Mustakim Billah ◽  
Abir Huzaifa ◽  
M. Abdul Kader Khan ◽  
Nusrat Jahan Vabna ◽  
Kashfia Nawrin ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Medicinal Value ◽  
Aqueous Leaf Extract ◽  
Anti Inflammatory ◽  
Vitro Analysis ◽  
In Vitro Analysis

Background: Crotalaria verrucosa is a traditional plant frequently prescribed by the tribes for its medicinal value against inflammation. The present study was designed to investigate the scientific basis for medicinal value in inflammation by in vivo and in vitro analysis.Methods: Anti-inflammatory activity of the plant’s leaf was evaluated by two in vivo methods - carrageenan induced rat paw edema and xylene induced mice ear edema. Moreover, in vitro analysis was performed through heat induced hemolysis and heat induced protein denaturation methods.Results: The inflammation produced by carrageenan and xylene were effectively suppressed by the aqueous leaf extract of C. verrucosa (CVAQ) at 600 mg/kg body weight which was comparable to the standards. In heat induced hemolysis test the extract was able to inhibit the lysis up to 70% at 500 µg/ml whereas in heat induced protein denaturation test it reduces the percentage till 69% at the same concentration.Conclusions: The findings suggested that CVAQ possess moderate to high anti-inflammatory activity when applied in low to high concentrated doses. However, the study can only conclude from this basic evaluation that the extract needs to be further investigated for identifying the potential compound which contributed to such medicinal value of the plant.

scholarly journals In Vitro and In Vivo Anti-Inflammatory Activity of Tetrastigma Sulcatum Leaf Extract, Pure Compound and Its Derivatives

2021 ◽  
Author(s):  
Ravindra Jagannath Waghole ◽  
Ashwini Vivek Misar ◽  
Neha Shashikant Kulkarni ◽  
Feroz Khan ◽  
Dattatraya Gopal Naik ◽  
...  
Keyword(s):  
Proinflammatory Cytokines ◽  
Crude Extract ◽  
Leaf Extract ◽  
Inflammatory Activity ◽  
No Production ◽  
Pure Compound ◽  
Tnf Α ◽  
Anti Inflammatory

Abstract The severity and perseverance of the inflammation have been demonstrated in many health conditions. The limitations of existing medications, propose the need for newer alternative anti-inflammatory medications. In our earlier studies, we demonstrated the topical anti-inflammatory potential of crude ethanolic extract of Tetrastigma sulcatum leaves and its fractions. In the present study, we further explored anti-inflammatory activity of T. sulcatum extract, fractions, pure compound and its derivatives using in vitro and in vivo bioassay techniques. We attempted to isolate a pure compound from leaf extract and was identified as Friedelan-3β-ol (CI) and its derivatives Friedelinol acetate (C II) and Friedelinol methyl ether (C III) were synthesized. Treatment with crude extract and its fractions demonstrated a significant reduction in the mRNA expression levels of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and nitric oxide (NO) production in LPS-stimulated inflammation in RAW 264.7 cells. Likewise, compounds CI, CII and CIII showed a similar pattern of significant inhibition of proinflammatory cytokines and NO production. In vivo study in Carrageenan induced paw-inflammatory mice model demonstrated reduced paw oedema and proinflammatory cytokines levels in a dose-dependent manner upon treatment of extract, its fractions, pure compound (CI), and their derivatives (CII and CIII.). The docking study showed all the compounds (CI, CII and CIII) share common residues with Dexamethasone. TNF- α exhibited the most interacting residues with the compounds. The present study confirmed the T. sulcatum ’s anti-inflammatory activity, suggesting Friedelan-3β-ol as an active component in a crude extract.

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