Improvement of Resistance of Wheat Cultivars to Fusarium Head Blight in the Yellow-Huai Rivers Valley Winter Wheat Zone with Functional Marker Selection of Fhb1 Gene

Aim. The aim of the work was to evaluate the genetic background of resistance to Fusarium head blight in common winter wheat cultivars based on the allelic state of the TDF_076_2D gene conferring tolerance against Fusarium graminearum Schwabe and F. culmorum (W.G.Sm.) Sacc. fungi. Methods. We studied 91 winter common wheat cultivars developed in the Institute of Plant Physiology and Genetics of NAS of Ukraine. A silica-based commercial kit was used for DNA extraction. For the allelic state detection, the INDEL1 marker co-segregating with the TDF_076_2D gene was used. Results. The frequency of the resistance allele according to the marker for the gene conferring moderate resistance to the Fusarium fungi made up 0.802. Conclusions. The majority of the common wheat cultivars from the studied sample carry the resistance allele of the gene of interest. The data obtained are consistent with the results of previous research for the wider sample of the winter and spring common wheat cultivars. The cultivars with confirmed resistance allele might show lower infection level in the field and serve as a source of the gene in marker assisted selection. Keywords: common wheat, disease resistance genes, Fusarium head blight, molecular markers.

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Assessment of Agrocenosis Adaptability of Winter Wheat in the Conditions of the Foothill Zone of the Central Caucasus

KnE Life Sciences ◽

10.18502/kls.v0i0.9025 ◽

2021 ◽

Author(s):

Irina Manukyan ◽

Elena Miroshnikova ◽

Madina Basieva ◽

Nino Doguzova

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Leaf Beetle ◽

Tan Spot ◽

Head Blight ◽

Wheat Varieties ◽

Cereal Leaf Beetle ◽

Oulema Melanopus ◽

Central Caucasus ◽

Selection Of

This paper presents the results of multi-year research on the adaptability of winter wheat varieties to the conditions of the foothill zone of the Central Caucasus. Characteristics such as ductility and stability were used to assess varieties for conformity to the cultivation conditions. Plant homeostaticity served as the indicator of ontogenetic adaptability. Phenological observations and reports were carried out according to the state variety testing methodology. The species composition of weed vegetation was established using the Agroecological Atlas of Russia. Conventional insect accounting methods were used to consider phytophages and plants damaged by them. It was established that the dominant pests of winter grains in the early periods of vegetation include phytophage bugs and cereal leaf beetle Oulema melanopus L. The maximum number of phytophage bugs was noted during the earing-flowering period. The proportion of pathogens of spike Fusarium head blight, tan spot and Septoria spot prevailed in the pathogenic complex. In total, 36.7% of varieties, including Kuma, Deya, Esaul, Starshina, Delta, Nota and Antonina, were medium-resistant to Fusarium head blight damage. According to the results of a comprehensive assessment of winter wheat selection by the parameters of adaptability, productivity and resistance to diseases, highly plastic genotypes responsive to environmental factors were distinguished: Kuma, Deya, Veda, Tanya, and List 25 (bi = 2.2; 2,3; 2,1). The selection of varieties was based on the adaptive properties of winter wheat. Fusarium head blight decreased by 20.8% in the mixed variety Batko+Deya, and the yield of mixed variety crops increased by 9%. Methods for compiling mixed varieties and designing the mosaics of varieties in the area of grain agrocenosis are recommended. Keywords: winter wheat, sustainability of agrocenosis, productivity, ecological plasticity, mixed varieties

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Native Fusarium head blight resistance from winter wheat cultivars ‘Lyman,’ ‘Overland,’ ‘Ernie,’ and ‘Freedom’ mapped and pyramided onto ‘Wesley’-Fhb1 backgrounds

Molecular Breeding ◽

10.1007/s11032-015-0200-1 ◽

2015 ◽

Vol 35 (1) ◽

Cited By ~ 11

Author(s):

Jonathan T. Eckard ◽

Jose L. Gonzalez-Hernandez ◽

Melanie Caffe ◽

William Berzonsky ◽

William W. Bockus ◽

...

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Fusarium Head Blight Resistance ◽

Blight Resistance ◽

Wheat Cultivars ◽

Head Blight ◽

Winter Wheat Cultivars

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Progression of Deoxynivalenol Concentrations in Spikes and Kernels of Winter Wheat Cultivars after Inoculation with Fusarium culmorum

Czech Journal of Genetics and Plant Breeding ◽

10.17221/3644-cjgpb ◽

2011 ◽

Vol 42 (No. 4) ◽

pp. 137-141 ◽

Cited By ~ 1

Author(s):

J. Chrpová ◽

V. Šíp ◽

E. Matějová ◽

S. Sýkorová

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Crucial Role ◽

Early Stage ◽

Fusarium Culmorum ◽

Wheat Cultivars ◽

Head Blight ◽

Winter Wheat Cultivars ◽

High Concentrations

Progression of deoxynivalenol (DON) concentrations in spikes and kernels was studied in relation to Fusarium head blight (FHB) symptoms in five winter wheat cultivars, differing in resistance to FHB, after single floret inoculation with an aggressive isolate of Fusarium culmorum. After inoculation in field conditions the spikes were detached from the plant and kept in the greenhouse under controlled conditions. High concentrations of DON were detected in susceptible cultivars at an early stage of pathogenesis (7 days after inoculation). Over the whole examined 21-day period and also at maturity spikes contained more DON than kernels. While differences between cultivars in the accumulation of DON were highly expressed already 7 days after inoculation, differences in symptomatic reactions were not clear until day 21. Owing to the reported crucial role of DON at early stages of pathogenesis, the importance of appropriate timing of fungicide application is highly stressed.  

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Meta-Analysis of Regression Coefficients for the Relationship Between Fusarium Head Blight and Deoxynivalenol Content of Wheat

Phytopathology ◽

10.1094/phyto-96-0951 ◽

2006 ◽

Vol 96 (9) ◽

pp. 951-961 ◽

Cited By ~ 64

Author(s):

P. A. Paul ◽

P. E. Lipps ◽

L. V. Madden

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Spring Wheat ◽

Meta Analysis ◽

Regression Coefficients ◽

Wheat Cultivars ◽

Head Blight ◽

Regression Slope ◽

Winter Wheat Cultivars ◽

The Relationship

A total of 126 field studies reporting deoxynivalenol (DON; ppm) content of harvested wheat grain and Fusarium head blight index (IND; field or plot-level disease severity) were analyzed to determine the overall mean regression slope and intercept for the relationship between DON and IND, and the influence of study-specific variables on the slope and intercept. A separate linear regression analysis was performed to determine the slope and intercept for each study followed by a meta-analysis of the regression coefficients from all studies. Between-study variances were significantly (P < 0.05) greater than 0, indicating substantial variation in the relationship between the variables. Regression slopes and intercepts were between -0.27 and 1.48 ppm per unit IND and -10.55 to 32.75 ppm, respectively. The overall mean regression slope and intercept, 0.22 ppm per unit IND and 2.94 ppm, respectively, were significantly different from zero (P < 0.001), and the width of the 95% confidence interval was 0.07 ppm per unit IND for slope and 1.44 ppm for intercept. Both slope and intercept were significantly affected by wheat type (P < 0.05); the overall mean intercept was significantly higher in studies conducted using winter wheat cultivars than in studies conducted using spring wheat cultivars, whereas the overall mean slope was significantly higher in studies conducted using spring wheat cultivars than in winter wheat cultivars. Study location had a significant effect on the intercept (P < 0.05), with studies from U.S. winter wheat-growing region having the highest overall mean intercept followed by studies from Canadian wheat-growing regions and U.S. spring wheat-growing regions. The study-wide magnitude of DON and IND had significant effects on one or both of the regression coefficients, resulting in considerable reduction in between-study variances. This indicates that, at least indirectly, environment affected the relationship between DON and IND.

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