scholarly journals Genetic background for moderate resistance against fusarium head blight among winter wheat developed in the Forrest Steppe of Ukraine

2020 ◽  
Vol 26 ◽  
pp. 96-100
Author(s):  
A. V. Karelov ◽  
N. A. Kozub ◽  
I. I. Kucheriavy ◽  
O. I. Sozinova ◽  
I. O. Sozinov ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Common Wheat ◽  
Genetic Background ◽  
Resistance Allele ◽  
Wheat Cultivars ◽  
Head Blight ◽  
Infection Level ◽  
Allelic State ◽  
Moderate Resistance

Aim. The aim of the work was to evaluate the genetic background of resistance to Fusarium head blight in common winter wheat cultivars based on the allelic state of the TDF_076_2D gene conferring tolerance against Fusarium graminearum Schwabe and F. culmorum (W.G.Sm.) Sacc. fungi. Methods. We studied 91 winter common wheat cultivars developed in the Institute of Plant Physiology and Genetics of NAS of Ukraine. A silica-based commercial kit was used for DNA extraction. For the allelic state detection, the INDEL1 marker co-segregating with the TDF_076_2D gene was used. Results. The frequency of the resistance allele according to the marker for the gene conferring moderate resistance to the Fusarium fungi made up 0.802. Conclusions. The majority of the common wheat cultivars from the studied sample carry the resistance allele of the gene of interest. The data obtained are consistent with the results of previous research for the wider sample of the winter and spring common wheat cultivars. The cultivars with confirmed resistance allele might show lower infection level in the field and serve as a source of the gene in marker assisted selection. Keywords: common wheat, disease resistance genes, Fusarium head blight, molecular markers.

scholarly journals Polymorphism of Ukrainian common winter wheat cultivars with respect to the molecular marker for the gene conferring moderate resistance against Fusarium head blight

2019 ◽  
pp. 87-99
Author(s):  
N. Kozub ◽  
O. Sozinova ◽  
A. Karelov ◽  
I. Sozinov ◽  
I. Kucheriavyi ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Common Wheat ◽  
Total Sample ◽  
Resistance Allele ◽  
Wheat Cultivars ◽  
Head Blight ◽  
Infection Level ◽  
Allelic State ◽  
Moderate Resistance ◽  
Breeding And Genetics

Goal. The goal of the work was to evaluate the genetic potential of resistance to Fusarium head blight of collections of winter common wheat cultivars based on the allelic state of the TDF_076_2D gene conferring moderate resistance against Fusarium graminearum Schwabe and F. culmorum (W.G.Sm.) Sacc. fungi. Materials and methods. We studied 68 winter common wheat cultivars developed in different scientific-breeding institutions of Ukraine (the Myronivka Remeslo Institute of Wheat of NAAS, the Plant Breeding and Genetics Institute (PBGI), the Рlant Production Institute nd. a. V. Ya. Yuryev of NAAS, Poltava State Agrarian Academy (PSAA)). A silica-based commercial kit was used for DNA extraction. To detect the allelic state of the resistance gene, the INDEL1 marker cosegregating with the TDF_076_2D gene was used. Amplified fragments obtained as a result of PCR were separated in 3% agarose gel and visualized with use of ethidium bromide. Results. The frequency of the resistance allele according to the INDEL1 marker for the gene conferring moderate resistance to the Fusarium fungi ranged from 0.682 in the sample of PBGI cultivars to 0.882 in the PSAA group; the frequency of the resistance allele in the total sample made up 0.782. It was found that the cultivar ‘Poliovyk’ showed a non-typical pattern of amplified bands, which possibly indicates the allele for susceptibility to Fusarium head blight. Conclusions. The majority of the common wheat cultivars from the studied sample carry the resistance allele of the marker for the gene of interest. The data obtained are consistent with the results of the previous research of the wider sample of the winter and spring common wheat cultivars. The cultivars with the resistance allele might show a lower infection level in the field and serve as a source of the gene in marker assisted selection. The cultivar ‘Poliovyk’ is worth more detailed studying with use of other molecular markers for the TDF_076_2D gene and (or) sequencing to find out a precise allelic state of the gene.

Fusarium Head Blight and Moniliformin Accumulation in Kernels of 18 Winter Wheat Cultivars Inoculated with Fusarium Avenaceum (3 Years Study)

1997 ◽  
Vol 25 (3) ◽  
pp. 673-675 ◽  
Author(s):  
Piotr Goliński ◽  
Marian Kostecki ◽  
Przemysław Kaptur ◽  
Slawomir Wojciechowski ◽  
Zygmunt Kaczmarek ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Fusarium Avenaceum ◽  
Wheat Cultivars ◽  
Head Blight ◽  
Winter Wheat Cultivars

scholarly journals Progression of Deoxynivalenol Concentrations in Spikes and Kernels of Winter Wheat Cultivars after Inoculation with Fusarium culmorum

2011 ◽  
Vol 42 (No. 4) ◽  
pp. 137-141 ◽  
Author(s):  
J. Chrpová ◽  
V. Šíp ◽  
E. Matějová ◽  
S. Sýkorová
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Crucial Role ◽  
Early Stage ◽  
Fusarium Culmorum ◽  
Wheat Cultivars ◽  
Head Blight ◽  
Winter Wheat Cultivars ◽  
High Concentrations

Progression of deoxynivalenol (DON) concentrations in spikes and kernels was studied in relation to Fusarium head blight (FHB) symptoms in five winter wheat cultivars, differing in resistance to FHB, after single floret inoculation with an aggressive isolate of Fusarium culmorum. After inoculation in field conditions the spikes were detached from the plant and kept in the greenhouse under controlled conditions. High concentrations of DON were detected in susceptible cultivars at an early stage of pathogenesis (7 days after inoculation). Over the whole examined 21-day period and also at maturity spikes contained more DON than kernels. While differences between cultivars in the accumulation of DON were highly expressed already 7 days after inoculation, differences in symptomatic reactions were not clear until day 21. Owing to the reported crucial role of DON at early stages of pathogenesis, the importance of appropriate timing of fungicide application is highly stressed.  

scholarly journals Meta-Analysis of Regression Coefficients for the Relationship Between Fusarium Head Blight and Deoxynivalenol Content of Wheat

2006 ◽  
Vol 96 (9) ◽  
pp. 951-961 ◽  
Author(s):  
P. A. Paul ◽  
P. E. Lipps ◽  
L. V. Madden
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Spring Wheat ◽  
Meta Analysis ◽  
Regression Coefficients ◽  
Wheat Cultivars ◽  
Head Blight ◽  
Regression Slope ◽  
Winter Wheat Cultivars ◽  
The Relationship

A total of 126 field studies reporting deoxynivalenol (DON; ppm) content of harvested wheat grain and Fusarium head blight index (IND; field or plot-level disease severity) were analyzed to determine the overall mean regression slope and intercept for the relationship between DON and IND, and the influence of study-specific variables on the slope and intercept. A separate linear regression analysis was performed to determine the slope and intercept for each study followed by a meta-analysis of the regression coefficients from all studies. Between-study variances were significantly (P < 0.05) greater than 0, indicating substantial variation in the relationship between the variables. Regression slopes and intercepts were between -0.27 and 1.48 ppm per unit IND and -10.55 to 32.75 ppm, respectively. The overall mean regression slope and intercept, 0.22 ppm per unit IND and 2.94 ppm, respectively, were significantly different from zero (P < 0.001), and the width of the 95% confidence interval was 0.07 ppm per unit IND for slope and 1.44 ppm for intercept. Both slope and intercept were significantly affected by wheat type (P < 0.05); the overall mean intercept was significantly higher in studies conducted using winter wheat cultivars than in studies conducted using spring wheat cultivars, whereas the overall mean slope was significantly higher in studies conducted using spring wheat cultivars than in winter wheat cultivars. Study location had a significant effect on the intercept (P < 0.05), with studies from U.S. winter wheat-growing region having the highest overall mean intercept followed by studies from Canadian wheat-growing regions and U.S. spring wheat-growing regions. The study-wide magnitude of DON and IND had significant effects on one or both of the regression coefficients, resulting in considerable reduction in between-study variances. This indicates that, at least indirectly, environment affected the relationship between DON and IND.

Association between yield loss and Fusarium head blight traits in resistant and susceptible winter wheat cultivars

2021 ◽  
Author(s):  
Radivoje Jevtić ◽  
Nina Skenderović ◽  
Vesna Župunski ◽  
Mirjana Lalošević ◽  
Branka Orbović ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Yield Loss ◽  
Wheat Cultivars ◽  
Head Blight ◽  
Winter Wheat Cultivars
Sign in / Sign up

Export Citation Format

Share Document