Optimization of two-dimensional high performance liquid chromatographic columns for highly efficient separation of intact proteins

2010 ◽  
Vol 28 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Guangfeng HONG ◽  
Mingxia GAO ◽  
Guoquan YAN ◽  
Xia GUAN ◽  
Qian TAO ◽  
...  
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Two Dimensional ◽  
Intact Proteins ◽  
Efficient Separation ◽  
Highly Efficient ◽  
Chromatographic Columns ◽  
Liquid Chromatographic

Care of Reverse Phase High Performance Liquid Chromatographic Columns When Injecting Extracts of Fat- or Oil-Based Preparations

1981 ◽  
Vol 64 (5) ◽  
pp. 1258-1258
Author(s):  
Fredric M Rabel
Keyword(s):  
High Performance ◽  
Simple Procedure ◽  
Pharmaceutical Preparations ◽  
High Performance Liquid Chromatographic ◽  
Column Efficiency ◽  
Reverse Phase ◽  
Analytical Column ◽  
Chromatographic Columns ◽  
Liquid Chromatographic

Abstract When analyzing extracts of pharmaceutical preparations, the chromatographer may place some of the base fats or oils onto the reverse phase analytical column with each injection. This is not a problem if they are washed free from the column periodically. If not cleaned off the column, column efficiency and selectivity can be lost forever. A simple procedure is described for washing columns.

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