Separation of racemic flavonoids by high performance liquid chromatography using chiral mobile phase additive

2013 ◽  
Vol 31 (5) ◽  
pp. 447 ◽  
Author(s):  
Yanan SUN ◽  
Tong LI ◽  
Chen MA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive

Resolution of ketoconazole enantiomers by high-performance liquid chromatography and inclusion complex formation between selector and enantiomers

2015 ◽  
Vol 69 (10) ◽  
Author(s):  
Bi-Zhu Sun ◽  
Kui He ◽  
Xiao-Ming Chen ◽  
Chun-Yan Chen ◽  
Zhao Wang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Volume Fraction ◽  
Factors Affecting ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive ◽  
Analytical Resolution ◽  
Uv Absorption Spectrum

AbstractThe analytical resolution of ketoconazole (KTZ) enantiomers was performed by high-performance liquid chromatography with sulphobutylether-β-cyclodextrin (SBE-β-CD) as a chiral mobile phase additive (chiral selector). Some important factors affecting the resolution of KTZ enantiomers were investigated. In addition, the molecular interaction between KTZ and SBE-β-CD was studied using the UV absorption spectrum and HPLC for an understanding of the resolution process. The results show that the type and concentration of the chiral mobile phase additive, the pH of the mobile phase and the volume fraction of methanol (φMeOH) in the mobile phase all have a clear influence on the resolution of KTZ enantiomers. Under optimal conditions, namely the use of 0.5 mmol L

Sign in / Sign up

Export Citation Format

Share Document