Construction of multiplex PCR in variety identification of Porphyra haitanensis “Z-26” based on SCAR markers

2013 ◽  
Vol 37 (5) ◽  
pp. 688 ◽  
Author(s):  
Ting WANG ◽  
Yan XU ◽  
Chaotian XIE ◽  
Dehua JI ◽  
Changsheng CHEN
Keyword(s):  
Multiplex Pcr ◽  
Variety Identification ◽  
Scar Markers ◽  
Porphyra Haitanensis

SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia

2002 ◽  
Vol 16 (1) ◽  
pp. 46-54 ◽  
Author(s):  
S. Manguin ◽  
P. Kengne ◽  
L. Sonnier ◽  
R. E. Harbach ◽  
V. Baimai ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Multiplex Pcr ◽  
Scar Markers ◽  
Anopheles Dirus

Genetic diversity of root-knot nematodes from Brazil and development of SCAR markers specific for the coffee-damaging species

Genome ◽  
2002 ◽  
Vol 45 (5) ◽  
pp. 862-870 ◽  
Author(s):  
Onivaldo Randig ◽  
Michel Bongiovanni ◽  
Regina M.D.G Carneiro ◽  
Philippe Castagnone-Sereno
Keyword(s):  
Genetic Diversity ◽  
Multiplex Pcr ◽  
Rapd Markers ◽  
Phylogenetic Analyses ◽  
The Other ◽  
Scar Markers ◽  
Meloidogyne Hapla ◽  
Root Knot Nematodes ◽  
Sequence Characterized Amplified Region ◽  
Scar Primers

RAPD markers were used to characterize the genetic diversity and relationships of root-knot nematodes (RKN) (Meloidogyne spp.) in Brazil. A high level of infraspecific polymorphism was detected in Meloidogyne arenaria, Meloidogyne exigua, and Meloidogyne hapla compared with the other species tested. Phylogenetic analyses showed that M. hapla and M. exigua are more closely related to one another than they are to the other species, and illustrated the early divergence of these meiotically reproducing species from the mitotic ones. To develop a PCR-based assay to specifically identify RKN associated with coffee, three RAPD markers were further transformed into sequence-characterized amplified region (SCAR) markers specific for M. exigua, Meloigogyne incognita and Meloidogyne paranaensis, respectively. After PCR using the SCAR primers, the initial polymorphism was retained as the presence or absence of amplification. Moreover, multiplex PCR using the three pairs of SCAR primers in a single reaction enabled the unambiguous identification of each species, even in mixtures. Therefore, it is concluded that the method developed here has potential for application in routine diagnostic procedures.Key words: diagnostic, multiplex PCR, phylogeny, RAPD, root-knot nematodes.

scholarly journals Development of SCAR Markers for the Authentication of Acori Rhizoma Based on the Analysis of RAPD and Multiplex-PCR

2011 ◽  
Vol 19 (3) ◽  
pp. 162-169 ◽  
Author(s):  
Byeong-Cheol Moon ◽  
Yun-Ui Ji ◽  
Young-Mi Lee ◽  
Jin-Mi Chun ◽  
A-Yeong Lee ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Scar Markers

Authentication of Akebia quinata DECNE. from its common adulterant medicinal plant species based on the RAPD-derived SCAR markers and multiplex-PCR

2014 ◽  
Vol 37 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Byeong Cheol Moon ◽  
Yunui Ji ◽  
Young Mi Lee ◽  
Young Min Kang ◽  
Ho Kyoung Kim
Keyword(s):  
Medicinal Plant ◽  
Multiplex Pcr ◽  
Plant Species ◽  
Medicinal Plant Species ◽  
Scar Markers

scholarly journals Development of RAPD-Derived SCAR Markers and Multiplex-PCR for Authentication of the Schisandrae Fructus

2013 ◽  
Vol 21 (3) ◽  
pp. 165-173 ◽  
Author(s):  
Young Mi Lee ◽  
Byeong Cheol Moon ◽  
Yunui Ji ◽  
Hyeong Seok Seo ◽  
Ho Kyoung Kim
Keyword(s):  
Multiplex Pcr ◽  
Scar Markers

A multiplex PCR-SSCP test to genotype bovine β-casein alleles A1 , A2 , A3 , B, and C

1999 ◽  
Vol 30 (4) ◽  
pp. 322-323 ◽  
Author(s):  
A. Barroso ◽  
S. Dunner ◽  
J. Ca·ón
Keyword(s):  
Multiplex Pcr

Multiplex PCR zum Nachweis disseminierter Tumorzellen (DTC) im Blut von Patientinnen mit Mammakarzinom

2005 ◽  
Vol 127 (03) ◽  
Author(s):  
J Rom ◽  
A Schneeeweiss ◽  
V Zieglschmid ◽  
C Hollmann ◽  
O Böcher ◽  
...  
Keyword(s):  
Multiplex Pcr

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

EVALUATION OF THE ISOLATION AND DETECTION METHODS FOR SALMONELLA SPP. FROM EGG SHELL CONTAMINATION USING MULTIPLEX PCR.

2015 ◽  
Vol 14 (1) ◽  
pp. 282-288
Author(s):  
Israa Adnan Ibraheam Al-Baghdady ◽  
Ashwak Bassim Jassim ◽  
Zainab Khudher Ahmed
Keyword(s):  
Multiplex Pcr ◽  
Detection Methods ◽  
Salmonella Spp ◽  
Egg Shell
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