MONITORING OF BIOLOGICAL ACTIVITY OF BETA-LACTAM ANTIBIOTICS RECEPTOR BLAR-CTD IN THE PROCESSES OF ITS HETEROLOGICAL EXPRESSION, PURIFICATION AND APPLICATION IN ANALYTICAL SYSTEMS

2021 ◽  
Vol 1 (19) ◽  
pp. 246-248
Author(s):  
P.A. Semizhon ◽  
H.P. Scheslenok ◽  
I.V. Harbachova ◽  
I.I. Vashkevich ◽  
O.V. Sviridov
Keyword(s):  
Biological Activity ◽  
High Purity ◽  
Test System ◽  
Biologically Active ◽  
Beta Lactam ◽  
Binding Ability ◽  
Immune Systems ◽  
Test Strips ◽  
Beta Lactam Antibiotics

To develop the technologies of beta-lactam antibiotics bioassays in foods, high purity beta-lactam receptor BlaR-CTD has been obtained and characterized. A test system for the quantitation of the biologically active and immunoreactive receptor in the processes of its heterological expression, isolation and reagent forms preparation was elaborated. The immunochemical properties and antibiotic binding ability of BlaR-CTD were investigated, and the constructions and application procedures of receptor-immune systems for the assay of beta-lactam antibiotics using microplates or chromatographic test-strips were proposed.

scholarly journals Simultaneous Determination of Different Antibiotic Residues in Bovine and in Porcine Kidneys by Solid-Phase Fluorescence Immunoassay

2003 ◽  
Vol 86 (2) ◽  
pp. 236-240 ◽  
Author(s):  
Lieve Okerman ◽  
Katia De Wasch ◽  
Jan Van Hoof ◽  
Walter Smedts
Keyword(s):  
Solid Phase ◽  
Kidney Tissue ◽  
Test System ◽  
Penicillin G ◽  
Fluorescence Immunoassay ◽  
Beta Lactam ◽  
Residue Detection ◽  
Beta Lactam Antibiotics ◽  
Minimal Sample

Abstract Parallux®, a solid-phase fluorescence immunoassay (SPFIA) developed for antibiotic residue detection in milk, was used for analysis of bovine and porcine kidney tissue. Four tetracyclines, 2 broad-spectrum cephalosporins, 3 beta-lactam antibiotics, and cephapirin were detected in one run after minimal sample preparation. This commercially available test system is designed as cartridges, each with a combination of 1–4 tests. One cartridge can be used to detect 4 analytes in the same sample, or 1 or 2 analytes in different samples. The cartridge with the combination tetracyclines–ceftiofur–penicillin–cephapirin was selected because tetracyclines, beta-lactam antibiotics as well as cephalosporins, are registered for oral or parenteral use in bovines and pigs in Europe. The test is qualitative and is recommended only for screening. Tetracycline, oxytetracycline, chlortetracycline, and doxycycline were easily detected at 300 ppb with the tetracyclines channel; ceftiofur at 1000 ppb and cefquinome at 200 ppb with the ceftiofur channel; penicillin G, ampicillin, and amoxicillin at 50 ppb with the penicillin channel; and cephapirin at 100 ppb with the cephapirin channel. These levels are equal to or lower than the corresponding maximal residue limits in kidney tissue. Cephalexin was not detected. The SPFIA test can be used as an alternative to classical inhibition tests and for post-screening inhibitor- positive kidneys, because it detects 3 specific groups of antibiotics, which enables selection of specific confirmatory methods for identification and quantification.

scholarly journals Studies on .BETA.-lactam antibiotics. IX. Synthesis and biological activity of a new orally active cephalosporin, cefixime (FK027).

1985 ◽  
Vol 38 (12) ◽  
pp. 1738-1751 ◽  
Author(s):  
HIDEAKI YAMANAKA ◽  
TOSHIYUKI CHIBA ◽  
KOHJI KAWABATA ◽  
HISASHI TAKASUGI ◽  
TAKASHI MASUGI ◽  
...  
Keyword(s):  
Biological Activity ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics ◽  
Orally Active

scholarly journals Studies on .BETA.-lactam antibiotics. XIV Synthesis and biological activity of the (E)-isomer of FK027.

1986 ◽  
Vol 34 (8) ◽  
pp. 3458-3464 ◽  
Author(s):  
KOHJI KAWABATA ◽  
KENJI MIYAI ◽  
HISASHI TAKASUGI ◽  
TAKAO TAKAYA
Keyword(s):  
Biological Activity ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics
Sign in / Sign up

Export Citation Format

Share Document