A Review of Detection of Antibiotic Residues in Food by Surface-Enhanced Raman Spectroscopy

Antibiotics, as veterinary drugs, have made extremely important contributions to disease prevention and treatment in the animal breeding industry. However, the accumulation of antibiotics in animal food due to their overuse during animal feeding is a frequent occurrence, which in turn would cause serious harm to public health when they are consumed by humans. Antibiotic residues in food have become one of the central issues in global food safety. As a safety measure, rapid and effective analytical approaches for detecting these residues must be implemented to prevent contaminated products from reaching the consumers. Traditional analytical methods, such as liquid chromatography, liquid chromatography mass spectrometry, and capillary electrophoresis, involve time-consuming sample preparation and complicated operation and require expensive instrumentation. By comparison, surface-enhanced Raman spectroscopy (SERS) has excellent sensitivity and remarkably enhanced target recognition. Thus, SERS has become a promising alternative analytical method for detecting antibiotic residues, as it can provide an ultrasensitive fingerprint spectrum for the rapid and noninvasive detection of trace analytes. In this study, we comprehensively review the recent progress and advances that have been achieved in the use of SERS in antibiotic residue detection. We introduce and discuss the basic principles of SERS. We then present the prospects and challenges in the use of SERS in the detection of antibiotics in food. Finally, we summarize and discuss the current problems and future trends in the detection of antibiotics in food.

Analysis of imidacloprid residues in mango, cowpea and water samples based on portable molecular imprinting sensors

Imidacloprid is a neonicotinoid insecticide widely used in the production and cultivation of crops. In recent years, the extensive use of imidacloprid in agricultural production has resulted in large amounts of pesticide residues in agricultural products and the environment. Therefore, it is necessary to establish a rapid, accurate, sensitive and convenient method for detecting imidacloprid pesticide residues to ensure the safety of agricultural products and the environment. To clarify how to use the molecular imprinting method for the electrochemical rapid residue detection of imidacloprid. This paper selected reduced graphene oxide and gold nanoparticles as modifiers modified on screen-printed carbon electrodes (SPCE) chitosan as a functional monomer, and imidacloprid as template molecule to prepare molecularly imprinted polymer, and applied this sensor to the residue detection of imidacloprid. The results showed that the concentration of imidacloprid showed a good linear relationship with the peak response current, and the detection limit of imidacloprid was 0.5 μM, while the sensor had good repeatability and interference resistance. The recoveries of imidacloprid spiked on three samples, mango, cowpea and water, were in the range of 90–110% (relative standard deviation, RSD<5%), which proved the practicality and feasibility of the assay established in this paper. The results of this paper can be used as a basis for the research on the detection of imidacloprid pesticide residues in food or environment.

Development and Characterization of Integrated Nano-Sensors for Organic Residues and pH Field Detection

Meeting global water quality standards is a real challenge to ensure that food crops and livestock are fit for consumption, as well as for human health in general. A major hurdle affecting the detection of pollutants in water reservoirs is the lapse of time between the sampling moment and the availability of the laboratory-based results. Here, we report the preparation, characterization, and performance assessment of an innovative sensor for the rapid detection of organic residue levels and pH in water samples. The sensor is based on carbonaceous nanomaterials (CNMs) coated with an intrinsically conductive polymer, polyaniline (PANI). Inverse emulsion polymerizations of aniline in the presence of carbon nanotubes (CNTs) or graphene were prepared and confirmed by thermogravimetric analysis and high-resolution scanning electron microscopy. Aminophenol and phenol were used as proxies for organic residue detection. The PANI/CNM nanocomposites were used to fabricate thin-film sensors. Of all the CNMs, the smallest limit of detection (LOD) was achieved for multi-walled CNT (MWCNT) with a LOD of 9.6 ppb for aminophenol and a very high linearity of 0.997, with an average sensitivity of 2.3 kΩ/pH at an acid pH. This high sensor performance can be attributed to the high homogeneity of the PANI coating on the MWCNT surface.

Control de residuos de antibióticos en leche cruda en una empresa lechera en Jalisco México: estudio retrospectivo

The current regulation in Mexico is explicit about the prohibition of the presence of antibiotic residues in raw cow's milk. Due to the impact of the presence of antibiotic residues in milk in the production of dairy products, some dairy companies have implemented their own programs to control antibiotic residues to avoid collection of contaminated material. The aim of this study was to analyze the results of the implementation of a program to control the presence of antibiotic residues in raw cow´s milk collected by a big local dairy industry. During the applications of this program, the frequency of contaminated milk containing the two main groups of antibiotics: β-lactams and tetracyclines, in 9 concentration facilities of a local dairy company, with an average size receiving 800,000- 1,000,000 L/day during 5 years. The antibiotic residue detection was performed using SNAP® Beta-Lactam Test and SNAP® Tetracycline Test simultaneously. During the first year, 222 tanks (2,955,538 L) were tested, of them 93 were positive for antibiotic residues (42.11%). In the fifth year, from 889 tanks containing 5,658,062 L only 13 were positive (1.46%), obtaining a reduction rate of 10.16% annual average. When the companies control the presence of inhibitor residues in the raw milk buy the milk, the farmers discard the contaminated milk; however, they sell it even with antibiotics when the buyers do not carry out controls, so this control measure becomes a solution. The results showed a significant decrease in contamination, being a useful solution to the problem of the appearance of antibiotic residues in milk in the raw material of the plant.