Genetic transformation of cabbage (Brassica oleracea var. capitata) with synthetic cry 1F gene to impart resistant to diamondback moth (Plutella xylostella)

2013 ◽  
Vol 15 (01) ◽  
pp. 3-9
Author(s):  
H.M. Mahadeva Swamy ◽  
S.N. Nagesha ◽  
Prakash M Navale ◽  
T.K.S. Gowda ◽  
R. Asokan ◽  
...  
2005 ◽  
Vol 40 (3) ◽  
pp. 280-290 ◽  
Author(s):  
Robert R. Farrar ◽  
Martin Shapiro

When we evaluated the nucleopolyhedrovirus of the diamondback moth, Plutella xylostella (L.) (PxMNPV), in small plots of collard, Brassica oleracea L., artificially infested with this insect, numbers of larvae were reduced in all tests. Further reductions were obtained with the addition of a fluorescent brightener (Blankophor P167®) in both tests in which it was included. Addition of an ultraviolet light protectant (photostabilized titanium dioxide) to PxMNPV also improved control, but only when the test was conducted in May and June. Titanium dioxide had no effect when the test was conducted in September. Addition of both materials together did not improve results compared with addition of Blankophor P167 alone. No virus treatment, however, was as effective as the insecticide spinosad.


2019 ◽  
Vol 3 (2) ◽  
pp. 1
Author(s):  
Wagner Farias Barbosa ◽  
Luis Moreira de Araujo Junior ◽  
Alixelhe Pacheco Damascena ◽  
Victor Luiz Souza Lima ◽  
Dirceu Pratissoli

The present study about the oviposition preference of the diamondback moth, <em>Plutella xylostella</em> (Linnaeus, 1758) (Lepidoptera: Plutellidae), can contribute to the determination of the location and time of release of parasitoids and predators or application of insecticides or bio-insecticides in the cabbage culture, <em>Brassica oleracea</em> Capitata group (Linnaeus, 1758) (Brassicales: Brassicaceae). Thus, aimed to this study was to determine the oviposition pattern of diamondback moth on three leaves locations and age of cabbage plants. The locations of the evaluated leaves were external, intermediate and internal. The evaluated plant ages were 20, 40, 60 and 80 days after transplanting. The eggs deposition behavior by diamondback moth was influenced by the development stage of the cabbage plant <em>F</em><sub>6, 96</sub> = 438.21; <em>p</em> = 0.05). The oviposition preference on the external leaves occurred on plants with 20 days old, on the intermediate leaves at 40 days and on the internal leaves at 60 and 80 days. The age with greater oviposition was at 40 days on the external and intermediate leaves; and at 60 days on the internal leaves. Therefore, the behavior of egg deposition of the diamondback moth was altered as the cabbage plant development.


Agrikultura ◽  
2017 ◽  
Vol 28 (1) ◽  
Author(s):  
Nenet Susniahti ◽  
Tarkus Suganda ◽  
Sudarjat Sudarjat ◽  
Danar Dono ◽  
Andhita Nadhirah

ABSTRACTReproduction, fecundity and period of each growth phase of Plutella xylostella on some species of crucifersPlutella xylostella L. is one of major pests of cabbage (Brassica oleracea). P. xylostella, also known as diamondback moth, is an oligophagous with limited host only a crucifers. The pest often causes damage on the plant in the low to high altitude of the land. It is able to attack cabbage leaf from seedling to harvesting. An alternative controlling method that can be done is by using potential parasitoid, Diadegma semiclausum. Its application can be done using conservation technique through environment modification such as planting ornamental plants as parasitoid food source and alternative host for P. xylostella. The research aimed to find alternative host for P. xylostella. Experiment was conducted at greenhouse of Department Plant Pests and Disease, Faculty of Agriculture, Universitas Padjadjaran. Experiment was carried out by observation method using Randomized Blocked Design consisted of five treatments and four replications. The result demonstrated that Rorippa indica and Cardamine hirsuta were alternative host for P. xylostella. The average number of eggs laid on R. indica and C. hirsuta were 226.75 and 216.25 eggs, respectively. Survival rate of P. xylostella infested on R. indica was 32.50% whereas the survival rate of P. xylostella infested on C. hirsute was 28.75%. It can be concluded that P. xylostella was able to spend its whole life cycle on both plants.Keywords: Crucifers, Fecundity, Growth phase, P. xylostella, ReproductionABSTRAKPlutella xylostella L. merupakan salah satu hama utama kubis (Brassica oleracea). P. xylostella, juga dikenal sebagai Diamondback moth, yang bersifat oligofag dengan inang terbatas hanya pada tanaman kubis-kubisan. Hama ini sering menyebabkan kerusakan tanaman pada dataran rendah dan tinggi. Hama ini dapat menyerang pertanaman kubis pada saat bibit sampai panen. Sebuah metode pengendalian alternatif yang bisa dilakukan adalah dengan menggunakan parasitoid, Diadegma semiclausum. Penerapannya dapat dilakukan dengan menggunakan teknik konservasi melalui modifikasi lingkungan seperti menanam tanaman hias sebagai sumber pakan untuk parasitoid dan inang alternatif untuk P. xylostella. Penelitian ini bertujuan untuk menemukan inang alternative dari P. xylostella. Percobaan dilakukan di rumah kaca Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran. Percobaan ini dilakukan dengan metode observasi menggunakan RancanganAcak Kelompok yang terdiri dari 4 perlakuan dengan 4 ulangan. Hasil penelitian menunjukkan bahwa Rorippa indica dan Cardamine hirsuta merupakan inang alternatif bagi P. xylostella. Rata-rata jumlah telur yang diletakan pada R. indica dan C. hirsuta adalah 226, 75 dan 216,25 butir. Kelulusan hidup P.xylostella yang di-infestasikan pada R. indica adalah 32,50% dan pada C. hirsuta adalah 28,75%. serta pada kedua jenis tanaman tersebut, P.xylostella dapat menyelesaikan seluruh siklus hidupnya.Kata Kunci : Cruciferae, Fase perkembangan, Fekunditas, P. xylostella, Reproduksi


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