Terahertz Characteristic Absorption Spectrometric Analysis of Homocysteine

SummaryBy means of neutron activation and gamma-ray spectrometry the concentrations in the human mandibular articular disc of the following elements have been determined: Na, Mn, Cu, Zn, Rb, Sr, Cd, W, and Au. The discs were obtained at necropsy from seven men and nine women, ranging in age from 56 to 71 years.The activation was carried out in a thermal neutron flux of about 1.7 XlO12 neutrons × cm−2 × sec.−1 for about 20 hours. A chemical group separationwas performed before the gamma-ray spectrometry. Quantitative data based on the dry weight of the cartilage samples were obtained by comparing the photo-peak area of the identified elements with those of appropriate standards.

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A Questionnaire Survey on General Status and Opinions about Clinical Mass Spectrometric Analysis in Korea (2018)

Laboratory Medicine Online ◽

10.3343/lmo.2019.9.3.161 ◽

2019 ◽

Vol 9 (3) ◽

pp. 161

Author(s):

Sung-Eun Cho ◽

Hyojin Chae ◽

Hyung-Doo Park ◽

Sail Chun ◽

Yong-Wha Lee ◽

...

Keyword(s):

Questionnaire Survey ◽

Mass Spectrometric ◽

Mass Spectrometric Analysis ◽

Spectrometric Analysis ◽

General Status

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Mechanisms of Dopant Depth Profile Modification During Mass Spectrometric Analysis of Multilayer Structure

Ukrainian Journal of Physics ◽

10.15407/ujpe60.06.0511 ◽

2015 ◽

Vol 60 (6) ◽

pp. 511-520 ◽

Cited By ~ 1

Author(s):

A.A. Efremov ◽

◽

V.G. Litovchenko ◽

V.P. Melnik ◽

O.S. Oberemok ◽

...

Keyword(s):

Depth Profile ◽

Multilayer Structure ◽

Mass Spectrometric ◽

Mass Spectrometric Analysis ◽

Spectrometric Analysis ◽

Profile Modification

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Mass Spectrometric Analysis of Porcine Rhodopsin¶

Photochemistry and Photobiology ◽

10.1562/0031-8655(2002)075<0316:msaopr>2.0.co;2 ◽

2002 ◽

Vol 75 (3) ◽

pp. 316 ◽

Cited By ~ 6

Author(s):

Zsolt Ablonczy ◽

Patrice Goletz ◽

Daniel R. Knapp ◽

Rosalie K. Crouch

Keyword(s):

Mass Spectrometric ◽

Mass Spectrometric Analysis ◽

Spectrometric Analysis

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THE DYNACTOME: INVESTIGATING DYNAMIC KINASE NETWORKS IN CELLULAR DECISION-MAKING

Clinical & Investigative Medicine ◽

10.25011/cim.v31i4.4827 ◽

2008 ◽

Vol 31 (4) ◽

pp. 22

Author(s):

Jonathan So ◽

Kelly Elder ◽

Anna Dai ◽

Claus Jorgensen ◽

Rune Linding ◽

...

Keyword(s):

Spectrometric Analysis ◽

Phosphorylation Sites ◽

Complex Samples ◽

Downstream Signaling ◽

Through Flow ◽

Colorectal Cancer Cells ◽

Induced Apoptosis ◽

Cell Phenotypes ◽

Kinase Phosphorylation ◽

Kinase Expression

Networks of kinases play a role in the transmission and integration of signals from the membrane to the nucleus. We aim to elucidate kinase phosphorylation and interaction partners in these networks through the immuno-precipitation and mass spectrometric analysis of a representative set of 100 Flag-tagged kinases stably expressed in human colorectal cancer cells. The goal is to generate a comprehensive set of interactions and dynamic phosphorylation sites which correlate with cell phenotypes such as apoptosis and proliferation. The techniques of mass-spectrometry have allowed for the identification of proteins and their phosphorylation sites in complex samples. Various labeling methods such as iTRAQ has enabled the relative quantification of these sites as afunction of time (White et al. PNAS, 2007). However, kinases usually work in the context of particular signaling stimuli. We aim to characterize the role of these over-expressed kinases in the context of Trail-induced apoptosis. This isparticularly relevant to tumorigenesis in that many cancers are resistant to apoptosis and recombinant Trail therapies are currently undergoing clinical trials. We present assays to correlate the proliferative ability and sensitivity to apoptosis of various stable cell lines with kinase expression levels through flow cytometry. We also present efforts to trace downstream signaling through the monitoring of MAP kinase phosphorylation using a high-throughput bead array.

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