scholarly journals Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

10.3791/53414 ◽  
2015 ◽  
Author(s):  
Nicholas Cormier ◽  
Alexander Yeo ◽  
Elizabeth Fiorentino ◽  
Julia Paxson
Keyword(s):  
Cell Migration ◽  
Cigarette Smoke ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Cigarette Smoke Extract ◽  
Scratch Assay ◽  
Wound Scratch Assay

Cigarette smoke extract reduces FOXO3a promoting tumor progression and cell migration in lung cancer

Toxicology ◽  
2021 ◽  
pp. 152751
Author(s):  
Serena Di Vincenzo ◽  
Claudia Sangiorgi ◽  
Maria Ferraro ◽  
Marco Buscetta ◽  
Chiara Cipollina ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Migration ◽  
Tumor Progression ◽  
Cigarette Smoke ◽  
Cigarette Smoke Extract

scholarly journals Osteoarthritic milieu and hypoxia exert specific effects on adipose mesenchymal stromal cell migration and cytokine receptor expression

2019 ◽  
Vol 27 ◽  
pp. S434-S435
Author(s):  
C. Manferdini ◽  
F. Paolella ◽  
E. Gabusi ◽  
D. Trucco ◽  
L. Cattini ◽  
...  
Keyword(s):  
Cell Migration ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Receptor Expression ◽  
Cytokine Receptor ◽  
Specific Effects

scholarly journals Nuclear ReceptorsNur77andNurr1Modulate Mesenchymal Stromal Cell Migration

2012 ◽  
Vol 21 (2) ◽  
pp. 228-238 ◽  
Author(s):  
Marijke W. Maijenburg ◽  
Christian Gilissen ◽  
Sara M. Melief ◽  
Marion Kleijer ◽  
Kees Weijer ◽  
...  
Keyword(s):  
Cell Migration ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell

Mesenchymal Stromal Cell Migration: Possibilities to Improve Cellular Therapy

2012 ◽  
Vol 21 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Marijke W. Maijenburg ◽  
C. Ellen van der Schoot ◽  
Carlijn Voermans
Keyword(s):  
Cell Migration ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Cellular Therapy

scholarly journals Cellular-Mesenchymal to Epithelial Transition Factor Upregulates Aquaporin 3 Expression in Human Breast Cancer Cells

2020 ◽  
Author(s):  
Fanshuang Zhu ◽  
Lijie Chen ◽  
Yixing Zhou ◽  
Jiaxiang An ◽  
Zenggui Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Human Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Scratch Assay ◽  
Aquaporin 3 ◽  
Mesenchymal To Epithelial Transition ◽  
Wound Scratch Assay

Abstract Background Aquaporin 3 (AQP3) and Cellular-mesenchymal to epithelial transition factor (c-Met) are both overexpressed in human breast cancer and highly related to proliferation and migration. However, it is still unclear whether c-Met is associated with AQP3. The study objective was to explore the relationship between c-Met and AQP3 in human breast cancer. Methods We used immunohistochemistry to determine the expression of AQP3 and c-Met in breast cancer tissue specimens and analysed whether there was any correlation between the two molecules. Real-time quantitative polymerase chain reaction and Western blotting were used to detect AQP3 expression in MCF-7 and MDA-MB-231 cells after human hepatocyte growth factor (hHGF) treatment and/or transfection with c-Met silencing small interfering (si)RNA. Additionally, cell migration was examined with a wound scratch assay. Results Based on the immunohistochemical results combined with the clinicopathological data of 59 breast cancer patients, c-Met exhibited a significant correlation with AQP3 in breast cancer tissue. Studies have shown that hHGF can increase c-Met phosphorylation, and we discovered that the hHGF-induced increase in AQP3 expression was dose dependent. However, after reducing c-Met expression with c-Met-specific siRNA, AQP3 expression decreased accordingly. Additionally, the wound scratch assay showed that AQP3-specific siRNA or c-Met-specific siRNA significantly inhibited breast cancer cell migration, which was promoted by hHGF. Conclusions Our study indicated that AQP3 expression was regulated by c-Met in human breast cancer. These discoveries may help us comprehend the mechanisms underlying breast cancer development and invasion and offer another possibility for targeted treatment of breast cancer.

scholarly journals Role of ECM/peptide coatings on SDF-1α triggered mesenchymal stromal cell migration from microcarriers for cell therapy

2015 ◽  
Vol 18 ◽  
pp. 59-67 ◽  
Author(s):  
Riccardo Levato ◽  
Josep A. Planell ◽  
Miguel A. Mateos-Timoneda ◽  
Elisabeth Engel
Keyword(s):  
Cell Migration ◽  
Cell Therapy ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Peptide Coatings

Specific effects of osteoarthritic milieu and hypoxic conditions on adipose mesenchymal stromal cell migration and cytokine receptors expression

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S94
Author(s):  
C. Manferdini ◽  
E. Gabusi ◽  
D. Trucco ◽  
M. Rojewski ◽  
H. Schrezenmeier ◽  
...  
Keyword(s):  
Cell Migration ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Cytokine Receptors ◽  
Hypoxic Conditions ◽  
Specific Effects

Direct GSK-3β Inhibition Enhances Mesenchymal Stromal Cell Migration by Increasing Expression of Beta-PIX and CXCR4

2013 ◽  
Vol 47 (2) ◽  
pp. 811-820 ◽  
Author(s):  
Young Seo Kim ◽  
Min Young Noh ◽  
Ji Young Kim ◽  
Hyun-Jeung Yu ◽  
Kyung Suk Kim ◽  
...  
Keyword(s):  
Cell Migration ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Gsk 3Β
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