scholarly journals DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

10.3791/56228 ◽  
2017 ◽  
Author(s):  
Eliza L. Lewis ◽  
Aaron M. Leconte
Keyword(s):  
Dna Polymerase ◽  
Gel Electrophoresis ◽  
Near Infrared ◽  
Polymerase Activity ◽  
Activity Assay

Inactivation of hepatitis B virus: evaluation of the efficacy of the disinfectant ‘Solprogel’ using a DNA-polymerase activity assay

1997 ◽  
Vol 36 (4) ◽  
pp. 305-312 ◽  
Author(s):  
A. Hernández ◽  
F.J. Belda ◽  
J. Domínguez ◽  
L. Matas ◽  
M. Giménez ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Polymerase ◽  
Polymerase Activity ◽  
Activity Assay ◽  
B Virus

scholarly journals Characterization of a novel DNA polymerase activity assay enabling sensitive, quantitative and universal detection of viable microbes

2012 ◽  
Vol 40 (14) ◽  
pp. e109-e109 ◽  
Author(s):  
Daniel R. Zweitzig ◽  
Nichol M. Riccardello ◽  
Bruce I. Sodowich ◽  
S. Mark O’Hara
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity ◽  
Activity Assay ◽  
Universal Detection

Target‐Activated DNA Polymerase Activity for Sensitive RNase H Activity Assay

2019 ◽  
Vol 14 (7) ◽  
pp. 1800645 ◽  
Author(s):  
Yujin Jung ◽  
Chang Y. Lee ◽  
Ki S. Park ◽  
Hyun G. Park
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity ◽  
Rnase H ◽  
Activity Assay

Facile and Sensitive Fluorescence Assay of DNA Polymerase Activity Using Cu2+ and Ascorbate as Signal Developers

2019 ◽  
Vol 4 (8) ◽  
pp. 2398-2403 ◽  
Author(s):  
Xingxing Zhang ◽  
Qiang Liu ◽  
Yan Jin ◽  
Baoxin Li
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity ◽  
Fluorescence Assay

Suggestive Evidence for an Oncorna-Virus-Specific DNA Polymerase from C-Type Particles of Bovine Leukosis

1974 ◽  
Vol 29 (1-2) ◽  
pp. 72-75 ◽  
Author(s):  
B. Dietzschold ◽  
O.R. Kaaden ◽  
S. Ueberschaer ◽  
F. Weiland ◽  
O. C. Straub
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity ◽  
Friend Virus ◽  
Leukaemia Virus ◽  
Virus Particles ◽  
Virus Rna ◽  
Feline Leukaemia Virus ◽  
Bovine Leukosis ◽  
Bovine Lymphocytes ◽  
Viral Preparation

Abstract Typical C-type oncorna virus particles as shown by electron microscopy have been purified from the supernatant of cultured lymphocytes from bovine leukosis. In the purified C-particle fraction a DNA-polymerase activity was detected. Using several synthetic RNA-or DNA-homopolymers and 70S Friend virus RNA the template response of this bovine leukosis cell particle DNA polymerase was compared with those of feline leukaemia virus DNA polymerase and DNA polymerase from normal bovine lymphocytes. The DNA polymerase detected in the viral preparation of bovine leukosis is suggested to be an oncorna-virus-specific enzyme.

scholarly journals Human Cytomegalovirus Inhibitor AL18 Also Possesses Activity against Influenza A and B Viruses

2012 ◽  
Vol 56 (11) ◽  
pp. 6009-6013 ◽  
Author(s):  
Giulia Muratore ◽  
Beatrice Mercorelli ◽  
Laura Goracci ◽  
Gabriele Cruciani ◽  
Paul Digard ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Dna Polymerase ◽  
Human Cytomegalovirus ◽  
Influenza A ◽  
Molecular Model ◽  
Polymerase Activity

ABSTRACTAL18, an inhibitor of human cytomegalovirus DNA polymerase, was serendipitously found to also block the interaction between the PB1 and PA polymerase subunits of influenza A virus. Furthermore, AL18 effectively inhibited influenza A virus polymerase activity and the overall replication of influenza A and B viruses. A molecular model to explain the binding of AL18 to both cytomegalovirus and influenza targets is proposed. Thus, AL18 represents an interesting lead for the development of new antivirals.

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