Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

10.3791/59868 ◽  
2019 ◽  
Author(s):  
Matthew J. Beucler ◽  
William E. Miller
Keyword(s):  
Epithelial Cells ◽  
Salivary Glands ◽  
In Vitro Growth ◽  
Salivary Epithelial Cells

scholarly journals Interleukin 1α and interleukin 6 promote the in vitro growth of both normal and neoplastic human cervical epithelial cells

1997 ◽  
Vol 75 (6) ◽  
pp. 855-859 ◽  
Author(s):  
G Castrilli ◽  
D Tatone ◽  
MG Diodoro ◽  
S Rosini ◽  
M Piantelli ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Interleukin 6 ◽  
In Vitro Growth ◽  
Interleukin 1Α ◽  
Cervical Epithelial Cells

In vitro growth and maintenance of two morphologically distinct populations of thymic epithelial cells

1985 ◽  
Vol 90 (2) ◽  
pp. 439-450 ◽  
Author(s):  
A.C. Nieburgs ◽  
P.T. Picciano ◽  
J.H. Korn ◽  
T. McCalister ◽  
C. Allred ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
In Vitro Growth

scholarly journals Diminished in vitro growth of epidermal epithelial cells in PSS

1976 ◽  
Vol 19 (5) ◽  
pp. 969-970
Author(s):  
Michael D. Parker ◽  
Grace P. Kerby
Keyword(s):  
Epithelial Cells ◽  
In Vitro Growth

scholarly journals P2Y2nucleotide receptor activation enhances the aggregation and self-organization of dispersed salivary epithelial cells

2014 ◽  
Vol 307 (1) ◽  
pp. C83-C96 ◽  
Author(s):  
Farid G. El-Sayed ◽  
Jean M. Camden ◽  
Lucas T. Woods ◽  
Mahmoud G. Khalafalla ◽  
Michael J. Petris ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Epithelial Cells ◽  
Rho Gtpase ◽  
Ex Vivo ◽  
Growth Factor Receptor ◽  
Cell Aggregation ◽  
Receptor Activation ◽  
Self Organization ◽  
Salivary Epithelial Cells

Hyposalivation resulting from salivary gland dysfunction leads to poor oral health and greatly reduces the quality of life of patients. Current treatments for hyposalivation are limited. However, regenerative medicine to replace dysfunctional salivary glands represents a revolutionary approach. The ability of dispersed salivary epithelial cells or salivary gland-derived progenitor cells to self-organize into acinar-like spheres or branching structures that mimic the native tissue holds promise for cell-based reconstitution of a functional salivary gland. However, the mechanisms involved in salivary epithelial cell aggregation and tissue reconstitution are not fully understood. This study investigated the role of the P2Y2nucleotide receptor (P2Y2R), a G protein-coupled receptor that is upregulated following salivary gland damage and disease, in salivary gland reconstitution. In vitro results with the rat parotid acinar Par-C10 cell line indicate that P2Y2R activation with the selective agonist UTP enhances the self-organization of dispersed salivary epithelial cells into acinar-like spheres. Other results indicate that the P2Y2R-mediated response is dependent on epidermal growth factor receptor activation via the metalloproteases ADAM10/ADAM17 or the α5β1integrin/Cdc42 signaling pathway, which leads to activation of the MAPKs JNK and ERK1/2. Ex vivo data using primary submandibular gland cells from wild-type and P2Y2R−/−mice confirmed that UTP-induced migratory responses required for acinar cell self-organization are mediated by the P2Y2R. Overall, this study suggests that the P2Y2R is a promising target for salivary gland reconstitution and identifies the involvement of two novel components of the P2Y2R signaling cascade in salivary epithelial cells, the α5β1integrin and the Rho GTPase Cdc42.

Effect of prostaglandin producing modulators on in vitro growth of buffalo uterine epithelial cells

2012 ◽  
Vol 77 (5) ◽  
pp. 1014-1020 ◽  
Author(s):  
S. Nandi ◽  
S. Mondal ◽  
I.J. Reddy
Keyword(s):  
Epithelial Cells ◽  
In Vitro Growth ◽  
Uterine Epithelial Cells

scholarly journals In vitro Growth and Differentiation of Epithelial Cells derived from Post-embryonic Hair Follicles

1982 ◽  
Vol 35 (1) ◽  
pp. 103 ◽  
Author(s):  
Dean R Hewish ◽  
Robert C Marshall
Keyword(s):  
Epithelial Cells ◽  
Electrophoretic Analysis ◽  
Hair Follicles ◽  
Culture Period ◽  
Fibroblast Cells ◽  
Cell Aggregates ◽  
In Vitro Growth ◽  
Cultured Cell ◽  
Cell Densities

Cell aggregates formed during the first 2 days of culture of cells derived from hair folIicle tissue of young rats. Aggregates occurred at an accelerated rate at higher cell densities, and contained a high proportion of epithelial cells although a variable proportion of esenchymal (fibroblast) cells were present. Citrulline was detected in the cultured cell proteins. Electrophoretic analysis of the proteins showed the presence of hair cortical keratin in the cultures, but these proteins were not synthesized during the culture period, in conflict with previous findings.

Quantification of in vitro growth and synthesis in rabbit middle ear epithelial cells

1996 ◽  
Vol 253 (7) ◽  
Author(s):  
L.P. Schousboe ◽  
S. Blegvad ◽  
T. Ovesen
Keyword(s):  
Epithelial Cells ◽  
Middle Ear ◽  
In Vitro Growth

Factors Affecting in Vitro Growth of Harvested Enterocytes

1992 ◽  
Vol 1 (4) ◽  
pp. 299-306 ◽  
Author(s):  
M. Santos ◽  
B. T. Nguyen ◽  
J.S. Thompson
Keyword(s):  
Epithelial Cells ◽  
Cell Viability ◽  
Cell Number ◽  
Cell Yield ◽  
Growth Media ◽  
In Vitro Growth ◽  
Factors Affecting ◽  
Initial Cell ◽  
Number Of Cells

Selective enterocyte transplantation may be an alternative to whole organ transplantation for increasing absorptive capacity. Our aim was to determine the effect of initial cell number and viability, proportion of intact crypts, and basement membrane components (BMC) on the in vitro growth of rabbit enterocytes. Enterocytes were harvested using warm trypsinization from ileal segments in 40 rabbits. Initial cell viability was 92 ± 4% (mean ± SD), cell yield was 7.7 ± 3.6 × 106 cells/cm, and there were 0.51 ± 0.33 crypts/100 cells. Initial cell viability correlated with cell yield (r = −0.508, p < 0.001) and % crypts (r = 0.313, p < 0.05). Cell yield also correlated with % crypts (r = −0.645, p < 0.001). Enterocytes (5 × 106) were incubated in growth media in plain or BMC coated growth culture vessels for 14 days. There was a correlation between both number of cells seeded (r = 0.824, p < 0.001) and cell viability (r = −0.696, p < 0.01) and % growth colonies containing epithelial cells at 14 days. Both total growth colonies (r = −0.565, p < 0.05) and colonies with epithelial cells (r = −0.589, p < 0.05) had a negative correlation with % crypts. Incubating cells in BMC coated vessels (n = 6) resulted in significantly more dispase liberated cells after 14 days than in plain vessels (n = 6) (6.6 ± 1.1 vs. 3.8 ± 1.0 × 106, p < 0.05) but viability was similar (97 ± 2% vs. 96 ± 2%). Diamine oxidase (DAO) activity was significantly increased in the growth media of cells cultured on BMC compared to controls (0.76 ± 0.24 vs. 0.43 ± 0.28 units, p < 0.05) although DAO act/cell was similar. Conclusions: 1) cell yield using warm trypsinization is increased at the expense of viability and intact crypts; 2) in vitro growth is increased by increasing the number of cells but not intact crypts seeded; 3) BMC increase the yield of cells with viability and maturity similar to those cultured in plain vessels.

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