Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

After intoxication of rabbits with certain substances such as convulsant agents (3-acetylpyridine), centrally acting drugs (reserpine), or toxic metal compounds (tetraethyl lead) a significant observation by phase microscope is the loss of contrast of the hippocampal mossy fiber layer. It has been suggested that this alteration, as well as changes seen with the electron microscope in the hippocampal mossy fiber boutons, may be related to a loss of neurotransmitters. The purpose of these experiments was to apply the OsO4-zinc-iodide staining technique to the study of these structural changes since it has been suggested that OsO4-zinc-iodide stain reacts with neurotransmitters (acetylcholine, catecholamines).Domestic New Zealand rabbits (2.5 to 3 kg) were used. Hippocampal tissue was removed from normal and experimental animals treated with 3-acetylpyridine (antimetabolite of nicotinamide), reserpine (anti- hypertensive/tranquilizer), or iproniazid (antidepressant/monamine oxidase inhibitor). After fixation in glutaraldehyde hippocampal tissue was treated with OsO4-zinc-iodide stain and further processed for phase and electron microscope studies.

Effect of Calcium on the Growth and Differentiation of Cultured Rat Esophageal Epithelial Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053383 ◽

1982 ◽

Vol 40 ◽

pp. 132-133

Author(s):

W.T. Gunning ◽

M.R. Marino ◽

M.S. Babcock ◽

G.D. Stoner

Keyword(s):

Epithelial Cells ◽

Cell Types ◽

Replication Rate ◽

Enzymatic Dissociation ◽

Growth Assay ◽

Epidermal Growth ◽

Fetal Bovine ◽

Esophageal Epithelial Cells ◽

Cellular Replication

The role of calcium in modulating cellular replication and differentiation has been described for various cell types. In the present study, the effects of Ca++ on the growth and differentiation of cultured rat esophageal epithelial cells was investigated.Epithelial cells were isolated from esophagi taken from 8 week-old male CDF rats by the enzymatic dissociation method of Kaighn. The cells were cultured in PFMR-4 medium supplemented with 0.25 mg/ml dialyzed fetal bovine serum, 5 ng/ml epidermal growth factor, 10-6 M hydrocortisone 10-6 M phosphoethanolamine, 10-6 M ethanolamine, 5 pg/ml insulin, 5 ng/ml transferrin, 10 ng/ml cholera toxin and 50 ng/ml garamycin at 36.5°C in a humidified atmosphere of 3% CO2 in air. At weekly intervals, the cells were subcultured with a solution containing 1% polyvinylpyrrolidone, 0.01% EGTA, and 0.05% trypsin. After various passages, the replication rate of the cells in PFMR-4 medium containing from 10-6 M to 10-3 M Ca++ was determined using a clonal growth assay.

Effect of 19 MHz RF radiation on neurotransmitters in mouse brain

PsycEXTRA Dataset ◽

10.1037/e536102008-001 ◽

1975 ◽

Cited By ~ 1

Author(s):

James H. Merritt ◽

James W. Frazer

Keyword(s):

Mouse Brain

NIH-supported Study Shows How Immune Cells Change Wiring of the Developing Mouse Brain

PsycEXTRA Dataset ◽

10.1037/e590962012-001 ◽

2012 ◽

Keyword(s):

Mouse Brain ◽

Immune Cells ◽

Developing Mouse Brain