scholarly journals Fluorescence in situ hybridization using 18S rRNA-targeted probe for specific detection of thraustochytrids (Labyrinthulomycetes)

2007 ◽  
Vol 2 (2) ◽  
pp. 91-97 ◽  
Author(s):  
YOSHITAKE TAKAO ◽  
YUJI TOMARU ◽  
KEIZO NAGASAKI ◽  
YUKARI SASAKURA ◽  
RINKA YOKOYAMA ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
18S Rrna ◽  
Specific Detection

scholarly journals Enumeration of Respiring Pseudomonas spp. in Milk within 6 Hours by Fluorescence In Situ Hybridization following Formazan Reduction

2005 ◽  
Vol 71 (5) ◽  
pp. 2748-2752 ◽  
Author(s):  
Akiko Kitaguchi ◽  
Nobuyasu Yamaguchi ◽  
Masao Nasu
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Vital Staining ◽  
Culture Method ◽  
Specific Detection ◽  
Tetrazolium Chloride ◽  
Conventional Culture ◽  
Rrna Sequences ◽  
Conventional Culture Method

ABSTRACT Respiring Pseudomonas spp. in milk were quantified within 6 h by fluorescence in situ hybridization (FISH) with vital staining. FISH with an oligonucleotide probe based on 16S rRNA sequences was used for the specific detection of Pseudomonas spp. at the single cell level. 5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) was used to estimate bacterial respiratory activity. The numbers of respiring Pseudomonas cells as determined by FISH with CTC staining (CTC-FISH) were almost the same or higher than the numbers of CFU as determined by the conventional culture method.

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