scholarly journals Allele-specific detection of nascent transcripts by fluorescence in situ hybridization reveals temporal and culture-induced changes in Igf2 imprinting during pre-implantation mouse development

2001 ◽  
Vol 6 (3) ◽  
pp. 249-259 ◽  
Author(s):  
Mizuki Ohno ◽  
Nao Aoki ◽  
Hiroyuki Sasaki
2005 ◽  
Vol 71 (5) ◽  
pp. 2748-2752 ◽  
Author(s):  
Akiko Kitaguchi ◽  
Nobuyasu Yamaguchi ◽  
Masao Nasu

ABSTRACT Respiring Pseudomonas spp. in milk were quantified within 6 h by fluorescence in situ hybridization (FISH) with vital staining. FISH with an oligonucleotide probe based on 16S rRNA sequences was used for the specific detection of Pseudomonas spp. at the single cell level. 5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) was used to estimate bacterial respiratory activity. The numbers of respiring Pseudomonas cells as determined by FISH with CTC staining (CTC-FISH) were almost the same or higher than the numbers of CFU as determined by the conventional culture method.


2007 ◽  
Vol 2 (2) ◽  
pp. 91-97 ◽  
Author(s):  
YOSHITAKE TAKAO ◽  
YUJI TOMARU ◽  
KEIZO NAGASAKI ◽  
YUKARI SASAKURA ◽  
RINKA YOKOYAMA ◽  
...  

2006 ◽  
Vol 175 (4S) ◽  
pp. 287-288 ◽  
Author(s):  
Juliann M. Dziubinski ◽  
Michael F. Sarosdy ◽  
Paul R. Kahn ◽  
Mark D. Ziffer ◽  
William R. Love ◽  
...  

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