Detection of Four Types of Genetically Modified Maize by Polymerase Chain Reaction and Immuno-Kit Methods

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
L.-C. Chiueh ◽  
Y.-L. Chen ◽  
J.-H. Yu ◽  
D.Y.-C. Shih
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Chain Reaction ◽  
Polymerase Chain

Quantitative real-time polymerase chain reaction for the presence of genetically-modified maize in breaded “ready-to-cook” food products

Food Control ◽  
2008 ◽  
Vol 19 (10) ◽  
pp. 1002-1005 ◽  
Author(s):  
Angela Di Pinto ◽  
Flora Alfano ◽  
Angela Giordano ◽  
Federico Capuano ◽  
Terio Valentina ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Genetically Modified ◽  
Food Products ◽  
Genetically Modified Maize ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Real-Time Quantitative Polymerase Chain Reaction Methods for Four Genetically Modified Maize Varieties and Maize DNA Content in Food

2002 ◽  
Vol 85 (3) ◽  
pp. 646-653 ◽  
Author(s):  
Peter D Brodmann ◽  
Evelyn C Ilg ◽  
Hélène Berthoud ◽  
André Herrmann
Keyword(s):  
European Union ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Genetically Modified ◽  
Detection Methods ◽  
Genetically Modified Maize ◽  
The European Union ◽  
Chain Reaction ◽  
Maize Varieties ◽  
Polymerase Chain

Abstract Quantitative detection methods are needed for enforcement of the recently introduced labeling threshold for genetically modified organisms (GMOs) in food ingredients. This labeling threshold, which is set to 1% in the European Union and Switzerland, must be applied to all approved GMOs. Four different varieties of maize are approved in the European Union: the insect-resistant Bt176 maize (Maximizer), Bt11 maize, Mon810 (YieldGard) maize, and the herbicide-tolerant T25 (Liberty Link™) maize. Because the labeling must be considered individually for each ingredient, a quantitation system for the endogenous maize content is needed in addition to the GMO-specific detection systems. Quantitative real-time polymerase chain reaction detection methods were developed for the 4 approved genetically modified maize varieties and for an endogenous maize (invertase) gene system.

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